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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Corynebacterium glutamicum Uncharacterized RNA methyltransferase Cgl1903/cg2084 (Cgl1903, cg2084) CSB-YP764461DWY
CSB-EP764461DWY
CSB-BP764461DWY
CSB-MP764461DWY
CSB-EP764461DWY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neurospora crassa Riboflavin kinase (fmn-1) CSB-YP764462NHA
CSB-EP764462NHA
CSB-BP764462NHA
CSB-MP764462NHA
CSB-EP764462NHA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Protochlamydia amoebophila DNA polymerase IV (dinB) CSB-YP764463PAAD
CSB-EP764463PAAD
CSB-BP764463PAAD
CSB-MP764463PAAD
CSB-EP764463PAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Protochlamydia amoebophila Malate dehydrogenase (mdh) CSB-YP764464PAAD
CSB-EP764464PAAD
CSB-BP764464PAAD
CSB-MP764464PAAD
CSB-EP764464PAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Protochlamydia amoebophila UDP-3-O-acylglucosamine N-acyltransferase (lpxD) CSB-YP764465PAAD
CSB-EP764465PAAD
CSB-BP764465PAAD
CSB-MP764465PAAD
CSB-EP764465PAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Protochlamydia amoebophila SsrA-binding protein (smpB) CSB-YP764466PAAD
CSB-EP764466PAAD
CSB-BP764466PAAD
CSB-MP764466PAAD
CSB-EP764466PAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Protochlamydia amoebophila ATP synthase subunit c (atpE), partial CSB-YP764467PAAD1
CSB-EP764467PAAD1
CSB-BP764467PAAD1
CSB-MP764467PAAD1
CSB-EP764467PAAD1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Protochlamydia amoebophila 50S ribosomal protein L36 (rpmJ) CSB-YP764468PAAD
CSB-EP764468PAAD
CSB-BP764468PAAD
CSB-MP764468PAAD
CSB-EP764468PAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Protochlamydia amoebophila Glycogen synthase (glgA) CSB-YP764469PAAD
CSB-EP764469PAAD
CSB-BP764469PAAD
CSB-MP764469PAAD
CSB-EP764469PAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Protochlamydia amoebophila 30S ribosomal protein S4 (rpsD) CSB-YP764470PAAD
CSB-EP764470PAAD
CSB-BP764470PAAD
CSB-MP764470PAAD
CSB-EP764470PAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Protochlamydia amoebophila Succinyl-CoA ligase [ADP-forming] subunit beta (sucC) CSB-YP764471PAAD
CSB-EP764471PAAD
CSB-BP764471PAAD
CSB-MP764471PAAD
CSB-EP764471PAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Protochlamydia amoebophila tRNA dimethylallyltransferase (miaA) CSB-YP764472PAAD
CSB-EP764472PAAD
CSB-BP764472PAAD
CSB-MP764472PAAD
CSB-EP764472PAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Protochlamydia amoebophila Protoheme IX farnesyltransferase (ctaB), partial CSB-YP764473PAAD
CSB-EP764473PAAD
CSB-BP764473PAAD
CSB-MP764473PAAD
CSB-EP764473PAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Protochlamydia amoebophila Acetyl-coenzyme A carboxylase carboxyl transferase subunit alpha (accA) CSB-YP764474PAAD
CSB-EP764474PAAD
CSB-BP764474PAAD
CSB-MP764474PAAD
CSB-EP764474PAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Protochlamydia amoebophila Thymidylate kinase (tmk) CSB-YP764475PAAD
CSB-EP764475PAAD
CSB-BP764475PAAD
CSB-MP764475PAAD
CSB-EP764475PAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Protochlamydia amoebophila Deoxycytidine triphosphate deaminase (dcd) CSB-YP764476PAAD
CSB-EP764476PAAD
CSB-BP764476PAAD
CSB-MP764476PAAD
CSB-EP764476PAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Protochlamydia amoebophila Protein-L-isoaspartate O-methyltransferase (pcm) CSB-YP764477PAAD
CSB-EP764477PAAD
CSB-BP764477PAAD
CSB-MP764477PAAD
CSB-EP764477PAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Protochlamydia amoebophila Putative Holliday junction resolvase (pc0823) CSB-YP764478PAAD
CSB-EP764478PAAD
CSB-BP764478PAAD
CSB-MP764478PAAD
CSB-EP764478PAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Protochlamydia amoebophila Translation initiation factor IF-2 (infB), partial CSB-YP764479PAAD
CSB-EP764479PAAD
CSB-BP764479PAAD
CSB-MP764479PAAD
CSB-EP764479PAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Protochlamydia amoebophila 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase (ispG), partial CSB-YP764480PAAD
CSB-EP764480PAAD
CSB-BP764480PAAD
CSB-MP764480PAAD
CSB-EP764480PAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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