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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Type IV secretion system protein ptlE homolog (ptlE), partial CSB-YP763661BIK1
CSB-EP763661BIK1
CSB-BP763661BIK1
CSB-MP763661BIK1
CSB-EP763661BIK1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sec-independent protein translocase protein TatA (tatA), partial CSB-YP763662BIK
CSB-EP763662BIK
CSB-BP763662BIK
CSB-MP763662BIK
CSB-EP763662BIK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Imidazoleglycerol-phosphate dehydratase (hisB) CSB-YP763663BIK
CSB-EP763663BIK
CSB-BP763663BIK
CSB-MP763663BIK
CSB-EP763663BIK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Hemin import ATP-binding protein HmuV (hmuV) CSB-YP763664BIK
CSB-EP763664BIK
CSB-BP763664BIK
CSB-MP763664BIK
CSB-EP763664BIK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant ATP synthase subunit alpha (atpA), partial CSB-YP763665BIK
CSB-EP763665BIK
CSB-BP763665BIK
CSB-MP763665BIK
CSB-EP763665BIK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Orotate phosphoribosyltransferase (pyrE) CSB-YP763666BIK
CSB-EP763666BIK
CSB-BP763666BIK
CSB-MP763666BIK
CSB-EP763666BIK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Phosphoribosylglycinamide formyltransferase 2 (purT) CSB-YP763667BIK
CSB-EP763667BIK
CSB-BP763667BIK
CSB-MP763667BIK
CSB-EP763667BIK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arginine biosynthesis bifunctional protein ArgJ (argJ) CSB-YP763668BIK
CSB-EP763668BIK
CSB-BP763668BIK
CSB-MP763668BIK
CSB-EP763668BIK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Monofunctional biosynthetic peptidoglycan transglycosylase (mtgA), partial CSB-YP763669BIK1
CSB-EP763669BIK1
CSB-BP763669BIK1
CSB-MP763669BIK1
CSB-EP763669BIK1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant N-acetyl-gamma-glutamyl-phosphate reductase (argC) CSB-YP763670BIK
CSB-EP763670BIK
CSB-BP763670BIK
CSB-MP763670BIK
CSB-EP763670BIK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Elongation factor G 2 (fusA2), partial CSB-YP763671BIK
CSB-EP763671BIK
CSB-BP763671BIK
CSB-MP763671BIK
CSB-EP763671BIK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Protein MraZ (mraZ) CSB-YP763672BIK
CSB-EP763672BIK
CSB-BP763672BIK
CSB-MP763672BIK
CSB-EP763672BIK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant tRNA 5-methylaminomethyl-2-thiouridine biosynthesis bifunctional protein MnmC (mnmC), partial CSB-YP763673BIK
CSB-EP763673BIK
CSB-BP763673BIK
CSB-MP763673BIK
CSB-EP763673BIK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pyridoxine/pyridoxamine 5'-phosphate oxidase (pdxH) CSB-YP763674BIK
CSB-EP763674BIK
CSB-BP763674BIK
CSB-MP763674BIK
CSB-EP763674BIK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant UDP-N-acetylenolpyruvoylglucosamine reductase (murB) CSB-YP763675BIK
CSB-EP763675BIK
CSB-BP763675BIK
CSB-MP763675BIK
CSB-EP763675BIK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Outer-membrane lipoprotein carrier protein (lolA) CSB-YP763676BIK
CSB-EP763676BIK
CSB-BP763676BIK
CSB-MP763676BIK
CSB-EP763676BIK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant NADH-quinone oxidoreductase subunit D (nuoD) CSB-YP763677BIK
CSB-EP763677BIK
CSB-BP763677BIK
CSB-MP763677BIK
CSB-EP763677BIK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Tryptophan synthase beta chain (trpB) CSB-YP763678BIK
CSB-EP763678BIK
CSB-BP763678BIK
CSB-MP763678BIK
CSB-EP763678BIK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acyl carrier protein (acpP) CSB-YP763679BIK
CSB-EP763679BIK
CSB-BP763679BIK
CSB-MP763679BIK
CSB-EP763679BIK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cell division protein FtsB (ftsB), partial CSB-YP763680BIK
CSB-EP763680BIK
CSB-BP763680BIK
CSB-MP763680BIK
CSB-EP763680BIK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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