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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Flagellar L-ring protein (flgH) CSB-YP763701BIK
CSB-EP763701BIK
CSB-BP763701BIK
CSB-MP763701BIK
CSB-EP763701BIK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Recombination protein RecR (recR) CSB-YP763702BIK
CSB-EP763702BIK
CSB-BP763702BIK
CSB-MP763702BIK
CSB-EP763702BIK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Uracil phosphoribosyltransferase (upp) CSB-YP763703BIK
CSB-EP763703BIK
CSB-BP763703BIK
CSB-MP763703BIK
CSB-EP763703BIK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Protein FdhD homolog (fdhD) CSB-YP763704BIK
CSB-EP763704BIK
CSB-BP763704BIK
CSB-MP763704BIK
CSB-EP763704BIK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dihydroxy-acid dehydratase 1 (ilvD1), partial CSB-YP763705BIK
CSB-EP763705BIK
CSB-BP763705BIK
CSB-MP763705BIK
CSB-EP763705BIK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bordetella bronchiseptica LexA repressor (lexA) CSB-YP763706BTY
CSB-EP763706BTY
CSB-BP763706BTY
CSB-MP763706BTY
CSB-EP763706BTY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bordetella bronchiseptica NADH-quinone oxidoreductase subunit H (nuoH), partial CSB-YP763707BTY
CSB-EP763707BTY
CSB-BP763707BTY
CSB-MP763707BTY
CSB-EP763707BTY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bordetella bronchiseptica Nicotinate phosphoribosyltransferase (pncB) CSB-YP763708BTY
CSB-EP763708BTY
CSB-BP763708BTY
CSB-MP763708BTY
CSB-EP763708BTY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bordetella bronchiseptica 3-oxoacyl-[acyl-carrier-protein] synthase 3 (fabH) CSB-YP763709BTY
CSB-EP763709BTY
CSB-BP763709BTY
CSB-MP763709BTY
CSB-EP763709BTY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bordetella bronchiseptica 2-dehydro-3-deoxyphosphooctonate aldolase (kdsA) CSB-YP763710BTY
CSB-EP763710BTY
CSB-BP763710BTY
CSB-MP763710BTY
CSB-EP763710BTY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bordetella bronchiseptica Chromosomal replication initiator protein DnaA (dnaA) CSB-YP763711BTY
CSB-EP763711BTY
CSB-BP763711BTY
CSB-MP763711BTY
CSB-EP763711BTY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bordetella bronchiseptica Arginine--tRNA ligase (argS), partial CSB-YP763712BTY
CSB-EP763712BTY
CSB-BP763712BTY
CSB-MP763712BTY
CSB-EP763712BTY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bordetella bronchiseptica Type IV secretion system protein ptlB homolog (ptlB), partial CSB-YP763713BTY1
CSB-EP763713BTY1
CSB-BP763713BTY1
CSB-MP763713BTY1
CSB-EP763713BTY1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bordetella bronchiseptica 1- (5-phosphoribosyl)-5-[ (5-phosphoribosylamino)methylideneamino] imidazole-4-carboxamide isomerase (hisA) CSB-YP763714BTY
CSB-EP763714BTY
CSB-BP763714BTY
CSB-MP763714BTY
CSB-EP763714BTY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bordetella bronchiseptica Bifunctional protein GlmU (glmU) CSB-YP763715BTY
CSB-EP763715BTY
CSB-BP763715BTY
CSB-MP763715BTY
CSB-EP763715BTY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bordetella bronchiseptica Anthranilate phosphoribosyltransferase (trpD) CSB-YP763716BTY
CSB-EP763716BTY
CSB-BP763716BTY
CSB-MP763716BTY
CSB-EP763716BTY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bordetella bronchiseptica ATP synthase gamma chain (atpG) CSB-YP763717BTY
CSB-EP763717BTY
CSB-BP763717BTY
CSB-MP763717BTY
CSB-EP763717BTY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bordetella bronchiseptica Aspartyl/glutamyl-tRNA (Asn/Gln) amidotransferase subunit B (gatB) CSB-YP763718BTY
CSB-EP763718BTY
CSB-BP763718BTY
CSB-MP763718BTY
CSB-EP763718BTY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bordetella bronchiseptica UPF0301 protein BB4405 (BB4405) CSB-YP763719BTY
CSB-EP763719BTY
CSB-BP763719BTY
CSB-MP763719BTY
CSB-EP763719BTY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bordetella bronchiseptica Ribosomal protein L11 methyltransferase (prmA) CSB-YP763720BTY
CSB-EP763720BTY
CSB-BP763720BTY
CSB-MP763720BTY
CSB-EP763720BTY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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