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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Rhodopseudomonas palustris 7-cyano-7-deazaguanine synthase (queC) CSB-YP764581RLU
CSB-EP764581RLU
CSB-BP764581RLU
CSB-MP764581RLU
CSB-EP764581RLU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodopseudomonas palustris Transcriptional repressor NrdR (nrdR) CSB-YP764582RLU
CSB-EP764582RLU
CSB-BP764582RLU
CSB-MP764582RLU
CSB-EP764582RLU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodopseudomonas palustris DNA-directed RNA polymerase subunit omega (rpoZ) CSB-YP764583RLU
CSB-EP764583RLU
CSB-BP764583RLU
CSB-MP764583RLU
CSB-EP764583RLU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodopseudomonas palustris Bifunctional enzyme IspD/IspF (ispDF) CSB-YP764584RLU
CSB-EP764584RLU
CSB-BP764584RLU
CSB-MP764584RLU
CSB-EP764584RLU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodopseudomonas palustris Leucyl/phenylalanyl-tRNA--protein transferase (aat) CSB-YP764585RLU
CSB-EP764585RLU
CSB-BP764585RLU
CSB-MP764585RLU
CSB-EP764585RLU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodopseudomonas palustris Ribosomal RNA large subunit methyltransferase E (rlmE) CSB-YP764586RLU
CSB-EP764586RLU
CSB-BP764586RLU
CSB-MP764586RLU
CSB-EP764586RLU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodopseudomonas palustris tRNA dimethylallyltransferase (miaA) CSB-YP764587RLU
CSB-EP764587RLU
CSB-BP764587RLU
CSB-MP764587RLU
CSB-EP764587RLU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodopseudomonas palustris Formyl-coenzyme A transferase (frc) CSB-YP764588RLU
CSB-EP764588RLU
CSB-BP764588RLU
CSB-MP764588RLU
CSB-EP764588RLU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodopseudomonas palustris Phosphonates import ATP-binding protein PhnC 2 (phnC2) CSB-YP764589RLU
CSB-EP764589RLU
CSB-BP764589RLU
CSB-MP764589RLU
CSB-EP764589RLU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodopseudomonas palustris Pyridoxine/pyridoxamine 5'-phosphate oxidase (pdxH) CSB-YP764590RLU
CSB-EP764590RLU
CSB-BP764590RLU
CSB-MP764590RLU
CSB-EP764590RLU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodopseudomonas palustris Protein TolB (tolB) CSB-YP764591RLU
CSB-EP764591RLU
CSB-BP764591RLU
CSB-MP764591RLU
CSB-EP764591RLU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodopseudomonas palustris 50S ribosomal protein L31 (rpmE) CSB-YP764592RLU
CSB-EP764592RLU
CSB-BP764592RLU
CSB-MP764592RLU
CSB-EP764592RLU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodopseudomonas palustris Protoheme IX farnesyltransferase (ctaB), partial CSB-YP764593RLU
CSB-EP764593RLU
CSB-BP764593RLU
CSB-MP764593RLU
CSB-EP764593RLU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodopseudomonas palustris Ribose 1,5-bisphosphate phosphokinase PhnN (phnN) CSB-YP764594RLU
CSB-EP764594RLU
CSB-BP764594RLU
CSB-MP764594RLU
CSB-EP764594RLU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodopseudomonas palustris 3-demethylubiquinone-9 3-methyltransferase (ubiG) CSB-YP764595RLU
CSB-EP764595RLU
CSB-BP764595RLU
CSB-MP764595RLU
CSB-EP764595RLU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodopseudomonas palustris Probable rRNA maturation factor (RPA0446) CSB-YP764596RLU
CSB-EP764596RLU
CSB-BP764596RLU
CSB-MP764596RLU
CSB-EP764596RLU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodopseudomonas palustris 2,3-bisphosphoglycerate-independent phosphoglycerate mutase (gpmI), partial CSB-YP764597RLU
CSB-EP764597RLU
CSB-BP764597RLU
CSB-MP764597RLU
CSB-EP764597RLU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodopseudomonas palustris Chaperone protein DnaK (dnaK), partial CSB-YP764598RLU
CSB-EP764598RLU
CSB-BP764598RLU
CSB-MP764598RLU
CSB-EP764598RLU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodopseudomonas palustris UPF0102 protein RPA0323 (RPA0323) CSB-YP764599RLU
CSB-EP764599RLU
CSB-BP764599RLU
CSB-MP764599RLU
CSB-EP764599RLU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodopseudomonas palustris Dephospho-CoA kinase (coaE) CSB-YP764600RLU
CSB-EP764600RLU
CSB-BP764600RLU
CSB-MP764600RLU
CSB-EP764600RLU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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