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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Methanococcus maripaludis Phosphoglucosamine mutase (glmM) CSB-YP764441MSB
CSB-EP764441MSB
CSB-BP764441MSB
CSB-MP764441MSB
CSB-EP764441MSB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis V-type ATP synthase beta chain (atpB) CSB-YP764442MSB
CSB-EP764442MSB
CSB-BP764442MSB
CSB-MP764442MSB
CSB-EP764442MSB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis Tryptophan synthase alpha chain (trpA) CSB-YP764443MSB
CSB-EP764443MSB
CSB-BP764443MSB
CSB-MP764443MSB
CSB-EP764443MSB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis Diaminopimelate epimerase (dapF) CSB-YP764444MSB
CSB-EP764444MSB
CSB-BP764444MSB
CSB-MP764444MSB
CSB-EP764444MSB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis Probable L-aspartate dehydrogenase (nadX) CSB-YP764445MSB
CSB-EP764445MSB
CSB-BP764445MSB
CSB-MP764445MSB
CSB-EP764445MSB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis O-phosphoserine--tRNA (Cys) ligase (sepS), partial CSB-YP764446MSB
CSB-EP764446MSB
CSB-BP764446MSB
CSB-MP764446MSB
CSB-EP764446MSB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis Malate dehydrogenase (mdh) CSB-YP764447MSB
CSB-EP764447MSB
CSB-BP764447MSB
CSB-MP764447MSB
CSB-EP764447MSB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis UPF0251 protein MMP0619 (MMP0619) CSB-YP764448MSB
CSB-EP764448MSB
CSB-BP764448MSB
CSB-MP764448MSB
CSB-EP764448MSB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis Orotidine 5'-phosphate decarboxylase (pyrF) CSB-YP764449MSB
CSB-EP764449MSB
CSB-BP764449MSB
CSB-MP764449MSB
CSB-EP764449MSB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis UPF0333 protein MMP0528 (MMP0528), partial CSB-YP764450MSB1
CSB-EP764450MSB1
CSB-BP764450MSB1
CSB-MP764450MSB1
CSB-EP764450MSB1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis Replication factor C small subunit (rfcS) CSB-YP764451MSB
CSB-EP764451MSB
CSB-BP764451MSB
CSB-MP764451MSB
CSB-EP764451MSB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis Translation initiation factor 2 subunit beta (eif2b) CSB-YP764452MSB
CSB-EP764452MSB
CSB-BP764452MSB
CSB-MP764452MSB
CSB-EP764452MSB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis Nucleoside diphosphate kinase (ndk) CSB-YP764453MSB
CSB-EP764453MSB
CSB-BP764453MSB
CSB-MP764453MSB
CSB-EP764453MSB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis Prefoldin subunit beta (pfdB) CSB-YP764454MSB
CSB-EP764454MSB
CSB-BP764454MSB
CSB-MP764454MSB
CSB-EP764454MSB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis Phosphoribosylformylglycinamidine synthase 1 (purQ) CSB-YP764455MSB
CSB-EP764455MSB
CSB-BP764455MSB
CSB-MP764455MSB
CSB-EP764455MSB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis Pyridoxal biosynthesis lyase pdxS (pdxS) CSB-YP764456MSB
CSB-EP764456MSB
CSB-BP764456MSB
CSB-MP764456MSB
CSB-EP764456MSB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis 50S ribosomal protein L31e (rpl31e) CSB-YP764457MSB
CSB-EP764457MSB
CSB-BP764457MSB
CSB-MP764457MSB
CSB-EP764457MSB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis GTP cyclohydrolase mptA (mptA) CSB-YP764458MSB
CSB-EP764458MSB
CSB-BP764458MSB
CSB-MP764458MSB
CSB-EP764458MSB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis Putative nickel-responsive regulator (MMP0020) CSB-YP764459MSB
CSB-EP764459MSB
CSB-BP764459MSB
CSB-MP764459MSB
CSB-EP764459MSB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Corynebacterium glutamicum 50S ribosomal protein L34 (rpmH) CSB-YP764460DWY
CSB-EP764460DWY
CSB-BP764460DWY
CSB-MP764460DWY
CSB-EP764460DWY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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