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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Mycoplasma mycoides subsp. mycoides SC 50S ribosomal protein L15 (rplO) CSB-YP764541MLS
CSB-EP764541MLS
CSB-BP764541MLS
CSB-MP764541MLS
CSB-EP764541MLS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma mycoides subsp. mycoides SC 30S ribosomal protein S11 (rpsK) CSB-YP764542MLS
CSB-EP764542MLS
CSB-BP764542MLS
CSB-MP764542MLS
CSB-EP764542MLS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma mycoides subsp. mycoides SC Tyrosine--tRNA ligase (tyrS) CSB-YP764543MLS
CSB-EP764543MLS
CSB-BP764543MLS
CSB-MP764543MLS
CSB-EP764543MLS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma mycoides subsp. mycoides SC Protein MraZ (mraZ) CSB-YP764544MLS
CSB-EP764544MLS
CSB-BP764544MLS
CSB-MP764544MLS
CSB-EP764544MLS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma mycoides subsp. mycoides SC S-adenosylmethionine synthase (metK) CSB-YP764545MLS
CSB-EP764545MLS
CSB-BP764545MLS
CSB-MP764545MLS
CSB-EP764545MLS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma mycoides subsp. mycoides SC DNA repair protein recO (recO) CSB-YP764546MLS
CSB-EP764546MLS
CSB-BP764546MLS
CSB-MP764546MLS
CSB-EP764546MLS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma mycoides subsp. mycoides SC Ribonuclease HII (rnhB) CSB-YP764547MLS
CSB-EP764547MLS
CSB-BP764547MLS
CSB-MP764547MLS
CSB-EP764547MLS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma mycoides subsp. mycoides SC Translation initiation factor IF-2 (infB), partial CSB-YP764548MLS
CSB-EP764548MLS
CSB-BP764548MLS
CSB-MP764548MLS
CSB-EP764548MLS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma mycoides subsp. mycoides SC Glycerol kinase (glpK), partial CSB-YP764549MLS
CSB-EP764549MLS
CSB-BP764549MLS
CSB-MP764549MLS
CSB-EP764549MLS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma mycoides subsp. mycoides SC 50S ribosomal protein L20 (rplT) CSB-YP764550MLS
CSB-EP764550MLS
CSB-BP764550MLS
CSB-MP764550MLS
CSB-EP764550MLS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma mycoides subsp. mycoides SC Guanylate kinase (gmk) CSB-YP764551MLS
CSB-EP764551MLS
CSB-BP764551MLS
CSB-MP764551MLS
CSB-EP764551MLS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma mycoides subsp. mycoides SC Exodeoxyribonuclease 7 large subunit (xseA) CSB-YP764552MLS
CSB-EP764552MLS
CSB-BP764552MLS
CSB-MP764552MLS
CSB-EP764552MLS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma mycoides subsp. mycoides SC tRNA modification GTPase MnmE (mnmE) CSB-YP764553MLS
CSB-EP764553MLS
CSB-BP764553MLS
CSB-MP764553MLS
CSB-EP764553MLS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma mycoides subsp. mycoides SC 30S ribosomal protein S6 (rpsF) CSB-YP764554MLS
CSB-EP764554MLS
CSB-BP764554MLS
CSB-MP764554MLS
CSB-EP764554MLS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neurospora crassa FAS1 domain-containing protein NCU02579 (B5K2.060, NCU02579) CSB-YP764555NHA
CSB-EP764555NHA
CSB-BP764555NHA
CSB-MP764555NHA
CSB-EP764555NHA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica Vacuolar iron transporter 1.1 (VIT1.1), partial CSB-YP764556OFG1
CSB-EP764556OFG1
CSB-BP764556OFG1
CSB-MP764556OFG1
CSB-EP764556OFG1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Uncharacterized PPE family protein PPE54 (ppe54), partial CSB-YP764557MVZ
CSB-EP764557MVZ
CSB-BP764557MVZ
CSB-MP764557MVZ
CSB-EP764557MVZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Schizosaccharomyces pombe Uncharacterized protein CPT2R1.02 (SPBCPT2R1.02) CSB-YP764558SXV
CSB-EP764558SXV
CSB-BP764558SXV
CSB-MP764558SXV
CSB-EP764558SXV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Hephaestin-like protein 1 (HEPHL1), partial CSB-YP764559HU
CSB-EP764559HU
CSB-BP764559HU
CSB-MP764559HU
CSB-EP764559HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Methylcytosine dioxygenase TET2 (TET2), partial CSB-YP764560HU
CSB-EP764560HU
CSB-BP764560HU
CSB-MP764560HU
CSB-EP764560HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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