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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Ajellomyces capsulata Autophagy protein 5 (ATG5) CSB-YP002293AUN
CSB-EP002293AUN
CSB-BP002293AUN
CSB-MP002293AUN
CSB-EP002293AUN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Botryotinia fuckeliana DNA-directed RNA polymerase III subunit rpc3 (rpc82), partial CSB-YP018345BVD
CSB-EP018345BVD
CSB-BP018345BVD
CSB-MP018345BVD
CSB-EP018345BVD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Botryotinia fuckeliana Exportin-T (los1), partial CSB-YP026226BVD
CSB-EP026226BVD
CSB-BP026226BVD
CSB-MP026226BVD
CSB-EP026226BVD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Botryotinia fuckeliana Structure-specific endonuclease subunit slx1 (slx1) CSB-YP009442BVD
CSB-EP009442BVD
CSB-BP009442BVD
CSB-MP009442BVD
CSB-EP009442BVD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Botryotinia fuckeliana Eukaryotic translation initiation factor 3 subunit K (BC1G_11363) CSB-YP007540BVD
CSB-EP007540BVD
CSB-BP007540BVD
CSB-MP007540BVD
CSB-EP007540BVD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Botryotinia fuckeliana Eukaryotic translation initiation factor 3 subunit D (BC1G_12797), partial CSB-YP007533BVD
CSB-EP007533BVD
CSB-BP007533BVD
CSB-MP007533BVD
CSB-EP007533BVD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Janthinobacterium sp. Succinyl-CoA ligase [ADP-forming] subunit beta (sucC) CSB-YP022917JAQ
CSB-EP022917JAQ
CSB-BP022917JAQ
CSB-MP022917JAQ
CSB-EP022917JAQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Janthinobacterium sp. tRNA dimethylallyltransferase (miaA) CSB-YP024529JAQ
CSB-EP024529JAQ
CSB-BP024529JAQ
CSB-MP024529JAQ
CSB-EP024529JAQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Janthinobacterium sp. Thymidylate kinase (tmk) CSB-YP007226JAQ
CSB-EP007226JAQ
CSB-BP007226JAQ
CSB-MP007226JAQ
CSB-EP007226JAQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Janthinobacterium sp. GMP synthase [glutamine-hydrolyzing] (guaA), partial CSB-YP009582JAQ
CSB-EP009582JAQ
CSB-BP009582JAQ
CSB-MP009582JAQ
CSB-EP009582JAQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Janthinobacterium sp. Elongation factor G (fusA), partial CSB-YP009373JAQ
CSB-EP009373JAQ
CSB-BP009373JAQ
CSB-MP009373JAQ
CSB-EP009373JAQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Janthinobacterium sp. ATP synthase subunit b (atpF), partial CSB-YP002358JAQ1
CSB-EP002358JAQ1
CSB-BP002358JAQ1
CSB-MP002358JAQ1
CSB-EP002358JAQ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Klebsiella pneumoniae subsp. pneumoniae Lipoyl synthase (lipA) CSB-YP012927KAX
CSB-EP012927KAX
CSB-BP012927KAX
CSB-MP012927KAX
CSB-EP012927KAX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Klebsiella pneumoniae subsp. pneumoniae Cysteine desulfurase (sufS), partial CSB-YP015769KAX
CSB-EP015769KAX
CSB-BP015769KAX
CSB-MP015769KAX
CSB-EP015769KAX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Klebsiella pneumoniae subsp. pneumoniae Thymidylate synthase (thyA) CSB-YP025393KAX
CSB-EP025393KAX
CSB-BP025393KAX
CSB-MP025393KAX
CSB-EP025393KAX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Klebsiella pneumoniae subsp. pneumoniae Glycine dehydrogenase [decarboxylating] (gcvP), partial CSB-YP009493KAX
CSB-EP009493KAX
CSB-BP009493KAX
CSB-MP009493KAX
CSB-EP009493KAX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Klebsiella pneumoniae subsp. pneumoniae ATP synthase subunit alpha (atpA), partial CSB-YP002344KAX
CSB-EP002344KAX
CSB-BP002344KAX
CSB-MP002344KAX
CSB-EP002344KAX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Klebsiella pneumoniae subsp. pneumoniae Glucose-6-phosphate isomerase (pgi), partial CSB-YP009717KAX
CSB-EP009717KAX
CSB-BP009717KAX
CSB-MP009717KAX
CSB-EP009717KAX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Alkaliphilus metalliredigens Porphobilinogen deaminase (hemC) CSB-YP010524AUP
CSB-EP010524AUP
CSB-BP010524AUP
CSB-MP010524AUP
CSB-EP010524AUP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Alkaliphilus metalliredigens GMP synthase [glutamine-hydrolyzing] (guaA), partial CSB-YP009582AUP
CSB-EP009582AUP
CSB-BP009582AUP
CSB-MP009582AUP
CSB-EP009582AUP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務,您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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