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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Triatoma infestans 40S ribosomal protein S3a CSB-YP020444TKM
CSB-EP020444TKM
CSB-BP020444TKM
CSB-MP020444TKM
CSB-EP020444TKM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Ubiquitin-like protein ATG12 (ATG12) CSB-YP002283STA
CSB-EP002283STA
CSB-BP002283STA
CSB-MP002283STA
CSB-EP002283STA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Transcription initiation factor TFIID subunit 4 (TAF4) CSB-YP023090STA
CSB-EP023090STA
CSB-BP023090STA
CSB-MP023090STA
CSB-EP023090STA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Nucleolar protein 58 (NOP58), partial CSB-YP015940STA
CSB-EP015940STA
CSB-BP015940STA
CSB-MP015940STA
CSB-EP015940STA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Enolase-phosphatase E1 (UTR4) CSB-YP007674STA
CSB-EP007674STA
CSB-BP007674STA
CSB-MP007674STA
CSB-EP007674STA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Cytoplasmic tRNA 2-thiolation protein 2 (NCS2) CSB-YP003313STA
CSB-EP003313STA
CSB-BP003313STA
CSB-MP003313STA
CSB-EP003313STA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Ubiquitin-like modifier-activating enzyme ATG7 (ATG7), partial CSB-YP002294STA
CSB-EP002294STA
CSB-BP002294STA
CSB-MP002294STA
CSB-EP002294STA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Alcohol dehydrogenase 4 (ADH4) CSB-YP001356STA
CSB-EP001356STA
CSB-BP001356STA
CSB-MP001356STA
CSB-EP001356STA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Vacuolar ATPase assembly integral membrane protein VMA21 (VMA21), partial CSB-YP025866STA1
CSB-EP025866STA1
CSB-BP025866STA1
CSB-MP025866STA1
CSB-EP025866STA1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Autophagy protein 5 (ATG5) CSB-YP002293STA
CSB-EP002293STA
CSB-BP002293STA
CSB-MP002293STA
CSB-EP002293STA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Methylthioribose-1-phosphate isomerase (MRI1) CSB-YP014799STA
CSB-EP014799STA
CSB-BP014799STA
CSB-MP014799STA
CSB-EP014799STA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Eukaryotic translation initiation factor 3 subunit G (TIF35) CSB-YP007536STA
CSB-EP007536STA
CSB-BP007536STA
CSB-MP007536STA
CSB-EP007536STA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae NADH-cytochrome b5 reductase 2 (MCR1), partial CSB-YP006319STA1
CSB-EP006319STA1
CSB-BP006319STA1
CSB-MP006319STA1
CSB-EP006319STA1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Myosin-3 (MYO3), partial CSB-YP015297STA
CSB-EP015297STA
CSB-BP015297STA
CSB-MP015297STA
CSB-EP015297STA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Exportin-T (LOS1), partial CSB-YP026226STA
CSB-EP026226STA
CSB-BP026226STA
CSB-MP026226STA
CSB-EP026226STA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 40S ribosomal protein S3a (BBOV_III001480) CSB-YP020444BPY
CSB-EP020444BPY
CSB-BP020444BPY
CSB-MP020444BPY
CSB-EP020444BPY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Danio rerio UPF0510 protein INM02 (zgc:171980), partial CSB-YP003478DIL1
CSB-EP003478DIL1
CSB-BP003478DIL1
CSB-MP003478DIL1
CSB-EP003478DIL1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sclerotinia sclerotiorum Pescadillo homolog (nop7), partial CSB-YP017791STG
CSB-EP017791STG
CSB-BP017791STG
CSB-MP017791STG
CSB-EP017791STG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sclerotinia sclerotiorum Nascent polypeptide-associated complex subunit alpha (egd2) CSB-YP015403STG
CSB-EP015403STG
CSB-BP015403STG
CSB-MP015403STG
CSB-EP015403STG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sclerotinia sclerotiorum NADH-cytochrome b5 reductase 2 (mcr1), partial CSB-YP006319STG1
CSB-EP006319STG1
CSB-BP006319STG1
CSB-MP006319STG1
CSB-EP006319STG1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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