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重組蛋白

產(chǎn)品名稱(chēng) 貨號(hào) 規(guī)格
Recombinant Cronobacter sakazakii ATP synthase subunit beta (atpD) CSB-YP002350CXL
CSB-EP002350CXL
CSB-BP002350CXL
CSB-MP002350CXL
CSB-EP002350CXL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cronobacter sakazakii Ferrochelatase (hemH) CSB-YP008579CXL
CSB-EP008579CXL
CSB-BP008579CXL
CSB-MP008579CXL
CSB-EP008579CXL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cronobacter sakazakii 6-phosphofructokinase (pfkA) CSB-YP017822CXL
CSB-EP017822CXL
CSB-BP017822CXL
CSB-MP017822CXL
CSB-EP017822CXL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cronobacter sakazakii Glycine dehydrogenase [decarboxylating] (gcvP), partial CSB-YP009493CXL
CSB-EP009493CXL
CSB-BP009493CXL
CSB-MP009493CXL
CSB-EP009493CXL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio harveyi Thymidylate synthase (thyA) CSB-YP025393VEA
CSB-EP025393VEA
CSB-BP025393VEA
CSB-MP025393VEA
CSB-EP025393VEA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio harveyi Phosphatidylserine decarboxylase proenzyme (psd) CSB-YP018033VEA
CSB-EP018033VEA
CSB-BP018033VEA
CSB-MP018033VEA
CSB-EP018033VEA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio harveyi Methionyl-tRNA formyltransferase (fmt) CSB-YP015150VEA
CSB-EP015150VEA
CSB-BP015150VEA
CSB-MP015150VEA
CSB-EP015150VEA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Francisella tularensis subsp. holarctica Elongation factor Ts (tsf) CSB-YP025124FDV
CSB-EP025124FDV
CSB-BP025124FDV
CSB-MP025124FDV
CSB-EP025124FDV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Francisella tularensis subsp. holarctica Phosphatidylserine decarboxylase proenzyme (psd) CSB-YP018033FDV
CSB-EP018033FDV
CSB-BP018033FDV
CSB-MP018033FDV
CSB-EP018033FDV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Francisella tularensis subsp. holarctica ATP synthase subunit beta (atpD) CSB-YP002350FDV
CSB-EP002350FDV
CSB-BP002350FDV
CSB-MP002350FDV
CSB-EP002350FDV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Roseiflexus castenholzii Methylthioribose-1-phosphate isomerase (mtnA) CSB-YP014799RIP
CSB-EP014799RIP
CSB-BP014799RIP
CSB-MP014799RIP
CSB-EP014799RIP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Roseiflexus castenholzii Ribonuclease 3 (rnc) CSB-YP019809RIP
CSB-EP019809RIP
CSB-BP019809RIP
CSB-MP019809RIP
CSB-EP019809RIP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Roseiflexus castenholzii Translation initiation factor IF-2 (infB), partial CSB-YP015164RIP
CSB-EP015164RIP
CSB-BP015164RIP
CSB-MP015164RIP
CSB-EP015164RIP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Roseiflexus castenholzii ATP synthase subunit delta (atpH) CSB-YP002355RIP
CSB-EP002355RIP
CSB-BP002355RIP
CSB-MP002355RIP
CSB-EP002355RIP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Roseiflexus castenholzii Methionyl-tRNA formyltransferase (fmt) CSB-YP015150RIP
CSB-EP015150RIP
CSB-BP015150RIP
CSB-MP015150RIP
CSB-EP015150RIP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Roseiflexus castenholzii Ribosome-recycling factor (frr) CSB-YP014941RIP
CSB-EP014941RIP
CSB-BP014941RIP
CSB-MP014941RIP
CSB-EP014941RIP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nematostella vectensis Probable lysosomal cobalamin transporter (v1g160387), partial CSB-YP012997NGO
CSB-EP012997NGO
CSB-BP012997NGO
CSB-MP012997NGO
CSB-EP012997NGO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nematostella vectensis Flap endonuclease 1 (FEN1) CSB-YP008585NGO
CSB-EP008585NGO
CSB-BP008585NGO
CSB-MP008585NGO
CSB-EP008585NGO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nematostella vectensis Probable imidazolonepropionase (amdhd1) CSB-YP001659NGO
CSB-EP001659NGO
CSB-BP001659NGO
CSB-MP001659NGO
CSB-EP001659NGO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nematostella vectensis 40S ribosomal protein S3a (v1g242621) CSB-YP020444NGO
CSB-EP020444NGO
CSB-BP020444NGO
CSB-MP020444NGO
CSB-EP020444NGO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶(hù)提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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