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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Oryza sativa subsp. japonica 14-3-3-like protein GF14-B (GF14B) CSB-YP771951OFG
CSB-EP771951OFG
CSB-BP771951OFG
CSB-MP771951OFG
CSB-EP771951OFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica WUSCHEL-related homeobox 4 (WOX4) CSB-YP771952OFG
CSB-EP771952OFG
CSB-BP771952OFG
CSB-MP771952OFG
CSB-EP771952OFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica Cyclin-dependent kinase G-2 (CDKG-2), partial CSB-YP771953OFG
CSB-EP771953OFG
CSB-BP771953OFG
CSB-MP771953OFG
CSB-EP771953OFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica Peptide methionine sulfoxide reductase A2-1 (MSRA2-1) CSB-YP771954OFG
CSB-EP771954OFG
CSB-BP771954OFG
CSB-MP771954OFG
CSB-EP771954OFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica PHD finger protein ALFIN-LIKE 4 (Os04g0444900, LOC_Os04g36730) CSB-YP771955OFG
CSB-EP771955OFG
CSB-BP771955OFG
CSB-MP771955OFG
CSB-EP771955OFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica Formin-like protein 12 (FH12), partial CSB-YP771956OFG
CSB-EP771956OFG
CSB-BP771956OFG
CSB-MP771956OFG
CSB-EP771956OFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bigelowiella natans Cytochrome b6-f complex iron-sulfur subunit, chloroplastic (petC), partial CSB-YP771957BGQ
CSB-EP771957BGQ
CSB-BP771957BGQ
CSB-MP771957BGQ
CSB-EP771957BGQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Petroselinum crispum Flavonol synthase/flavanone 3-hydroxylase (FLS) CSB-YP771958PGL
CSB-EP771958PGL
CSB-BP771958PGL
CSB-MP771958PGL
CSB-EP771958PGL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica Protein argonaute 12 (AGO12), partial CSB-YP771959OFG
CSB-EP771959OFG
CSB-BP771959OFG
CSB-MP771959OFG
CSB-EP771959OFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica SPX domain-containing protein 5 (SPX5) CSB-YP771960OFG
CSB-EP771960OFG
CSB-BP771960OFG
CSB-MP771960OFG
CSB-EP771960OFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica DEAD-box ATP-dependent RNA helicase 10 (Os03g0669000, LOC_Os03g46610) CSB-YP771961OFG
CSB-EP771961OFG
CSB-BP771961OFG
CSB-MP771961OFG
CSB-EP771961OFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica Chitinase 7 (Cht7) CSB-YP771962OFG
CSB-EP771962OFG
CSB-BP771962OFG
CSB-MP771962OFG
CSB-EP771962OFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterobacteria phage RB69 Deoxynucleotide monophosphate kinase (1) CSB-YP771963ELM
CSB-EP771963ELM
CSB-BP771963ELM
CSB-MP771963ELM
CSB-EP771963ELM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Apomys hylocoetes Cytochrome b (MT-CYB), partial CSB-YP771964AGAW
CSB-EP771964AGAW
CSB-BP771964AGAW
CSB-MP771964AGAW
CSB-EP771964AGAW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chrysochloris asiatica NADH-ubiquinone oxidoreductase chain 4L (MT-ND4L), partial CSB-YP771965CIAS1
CSB-EP771965CIAS1
CSB-BP771965CIAS1
CSB-MP771965CIAS1
CSB-EP771965CIAS1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Myotis formosus Cytochrome b (MT-CYB), partial CSB-YP771966MHAA
CSB-EP771966MHAA
CSB-BP771966MHAA
CSB-MP771966MHAA
CSB-EP771966MHAA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Apomys gracilirostris Cytochrome b (MT-CYB), partial CSB-YP771967AGAX
CSB-EP771967AGAX
CSB-BP771967AGAX
CSB-MP771967AGAX
CSB-EP771967AGAX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lonchophylla chocoana Cytochrome b (MT-CYB), partial CSB-YP771968LDAI
CSB-EP771968LDAI
CSB-BP771968LDAI
CSB-MP771968LDAI
CSB-EP771968LDAI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces servazzii Cytochrome b (COB), partial CSB-YP771969SVU
CSB-EP771969SVU
CSB-BP771969SVU
CSB-MP771969SVU
CSB-EP771969SVU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Calycanthus floridus var. glaucus 30S ribosomal protein S15, chloroplastic (rps15) CSB-YP771970CBM
CSB-EP771970CBM
CSB-BP771970CBM
CSB-MP771970CBM
CSB-EP771970CBM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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