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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Uncharacterized RNA methyltransferase BA_0426/GBAA_0426/BAS0414 (BA_0426, GBAA_0426, BAS0414) CSB-YP772372BQE
CSB-EP772372BQE
CSB-BP772372BQE
CSB-MP772372BQE
CSB-EP772372BQE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methylthioribose-1-phosphate isomerase 1 (mtnA1) CSB-YP772373BQE
CSB-EP772373BQE
CSB-BP772373BQE
CSB-MP772373BQE
CSB-EP772373BQE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant DNA ligase (ligA), partial CSB-YP772374BQE
CSB-EP772374BQE
CSB-BP772374BQE
CSB-MP772374BQE
CSB-EP772374BQE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nitrosomonas europaea Putative phosphotransferase NE2358/NE2365 (NE2358) CSB-YP772375NHH
CSB-EP772375NHH
CSB-BP772375NHH
CSB-MP772375NHH
CSB-EP772375NHH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlamydophila caviae Demethylmenaquinone methyltransferase (ubiE) CSB-YP772376DSL
CSB-EP772376DSL
CSB-BP772376DSL
CSB-MP772376DSL
CSB-EP772376DSL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nitrosomonas europaea Glutamate-ammonia-ligase adenylyltransferase (glnE), partial CSB-YP772377NHH
CSB-EP772377NHH
CSB-BP772377NHH
CSB-MP772377NHH
CSB-EP772377NHH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rotavirus A Non-structural glycoprotein 4, partial CSB-YP772378RGU
CSB-EP772378RGU
CSB-BP772378RGU
CSB-MP772378RGU
CSB-EP772378RGU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rotavirus A Non-structural protein 1 CSB-YP772379RGU
CSB-EP772379RGU
CSB-BP772379RGU
CSB-MP772379RGU
CSB-EP772379RGU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rotavirus A Non-structural protein 3 CSB-YP772380ROH
CSB-EP772380ROH
CSB-BP772380ROH
CSB-MP772380ROH
CSB-EP772380ROH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nitrosomonas europaea Prolipoprotein diacylglyceryl transferase (lgt), partial CSB-YP772381NHH1
CSB-EP772381NHH1
CSB-BP772381NHH1
CSB-MP772381NHH1
CSB-EP772381NHH1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Tropheryma whipplei Prolipoprotein diacylglyceryl transferase (lgt), partial CSB-YP772382TIW1
CSB-EP772382TIW1
CSB-BP772382TIW1
CSB-MP772382TIW1
CSB-EP772382TIW1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Tropheryma whipplei DNA-directed RNA polymerase subunit omega (rpoZ) CSB-YP772383TIW
CSB-EP772383TIW
CSB-BP772383TIW
CSB-MP772383TIW
CSB-EP772383TIW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlamydophila caviae tRNA (guanine-N (1)-)-methyltransferase (trmD) CSB-YP772384DSL
CSB-EP772384DSL
CSB-BP772384DSL
CSB-MP772384DSL
CSB-EP772384DSL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlamydophila caviae (3R)-hydroxymyristoyl-[acyl-carrier-protein] dehydratase (fabZ) CSB-YP772385DSL
CSB-EP772385DSL
CSB-BP772385DSL
CSB-MP772385DSL
CSB-EP772385DSL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant ATP-dependent Clp protease ATP-binding subunit ClpX (clpX) CSB-YP772386SNN
CSB-EP772386SNN
CSB-BP772386SNN
CSB-MP772386SNN
CSB-EP772386SNN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nitrosomonas europaea Gamma-glutamyl phosphate reductase (proA) CSB-YP772387NHH
CSB-EP772387NHH
CSB-BP772387NHH
CSB-MP772387NHH
CSB-EP772387NHH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nitrosomonas europaea tRNA (guanine-N (1)-)-methyltransferase (trmD) CSB-YP772388NHH
CSB-EP772388NHH
CSB-BP772388NHH
CSB-MP772388NHH
CSB-EP772388NHH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nitrosomonas europaea Acetylglutamate kinase (argB) CSB-YP772389NHH
CSB-EP772389NHH
CSB-BP772389NHH
CSB-MP772389NHH
CSB-EP772389NHH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nitrosomonas europaea Deoxycytidine triphosphate deaminase (dcd) CSB-YP772390NHH
CSB-EP772390NHH
CSB-BP772390NHH
CSB-MP772390NHH
CSB-EP772390NHH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nitrosomonas europaea 30S ribosomal protein S3 (rpsC) CSB-YP772391NHH
CSB-EP772391NHH
CSB-BP772391NHH
CSB-MP772391NHH
CSB-EP772391NHH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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