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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Ribonuclease M5 (rnmV) CSB-YP772352BQE
CSB-EP772352BQE
CSB-BP772352BQE
CSB-MP772352BQE
CSB-EP772352BQE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Serine--tRNA ligase (serS) CSB-YP772353BQE
CSB-EP772353BQE
CSB-BP772353BQE
CSB-MP772353BQE
CSB-EP772353BQE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant UPF0348 protein BA_4135/GBAA_4135/BAS3837 (BA_4135, GBAA_4135, BAS3837) CSB-YP772354BQE
CSB-EP772354BQE
CSB-BP772354BQE
CSB-MP772354BQE
CSB-EP772354BQE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,6-diaminopimelate ligase (murE) CSB-YP772355BQE
CSB-EP772355BQE
CSB-BP772355BQE
CSB-MP772355BQE
CSB-EP772355BQE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cell division protein SepF (sepF) CSB-YP772356BQE
CSB-EP772356BQE
CSB-BP772356BQE
CSB-MP772356BQE
CSB-EP772356BQE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dihydroorotate dehydrogenase B (NAD (+)), catalytic subunit (pyrD) CSB-YP772357BQE
CSB-EP772357BQE
CSB-BP772357BQE
CSB-MP772357BQE
CSB-EP772357BQE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Peptide deformylase 1 (def1) CSB-YP772358BQE
CSB-EP772358BQE
CSB-BP772358BQE
CSB-MP772358BQE
CSB-EP772358BQE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Putative ribosome biogenesis GTPase RsgA (rsgA) CSB-YP772359BQE
CSB-EP772359BQE
CSB-BP772359BQE
CSB-MP772359BQE
CSB-EP772359BQE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ribosome maturation factor RimM (rimM) CSB-YP772360BQE
CSB-EP772360BQE
CSB-BP772360BQE
CSB-MP772360BQE
CSB-EP772360BQE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 30S ribosomal protein S2 (rpsB) CSB-YP772361BQE
CSB-EP772361BQE
CSB-BP772361BQE
CSB-MP772361BQE
CSB-EP772361BQE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Translation initiation factor IF-2 (infB), partial CSB-YP772362BQE
CSB-EP772362BQE
CSB-BP772362BQE
CSB-MP772362BQE
CSB-EP772362BQE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant (Dimethylallyl)adenosine tRNA methylthiotransferase MiaB (miaB), partial CSB-YP772363BQE
CSB-EP772363BQE
CSB-BP772363BQE
CSB-MP772363BQE
CSB-EP772363BQE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant UvrABC system protein B (uvrB), partial CSB-YP772364BQE
CSB-EP772364BQE
CSB-BP772364BQE
CSB-MP772364BQE
CSB-EP772364BQE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant ATP-dependent Clp protease proteolytic subunit 2 (clpP2) CSB-YP772365BQE
CSB-EP772365BQE
CSB-BP772365BQE
CSB-MP772365BQE
CSB-EP772365BQE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Protein CrcB homolog 1 (crcB1), partial CSB-YP772366BQE1
CSB-EP772366BQE1
CSB-BP772366BQE1
CSB-MP772366BQE1
CSB-EP772366BQE1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant L-lactate dehydrogenase 3 (ldh3) CSB-YP772367BQE
CSB-EP772367BQE
CSB-BP772367BQE
CSB-MP772367BQE
CSB-EP772367BQE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ferredoxin--NADP reductase 2 (BA_5160, GBAA_5160, BAS4797) CSB-YP772368BQE
CSB-EP772368BQE
CSB-BP772368BQE
CSB-MP772368BQE
CSB-EP772368BQE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant UPF0154 protein BA_3742/GBAA_3742/BAS3468 (BA_3742, GBAA_3742, BAS3468), partial CSB-YP772369BQE
CSB-EP772369BQE
CSB-BP772369BQE
CSB-MP772369BQE
CSB-EP772369BQE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Small, acid-soluble spore protein N (sspN) CSB-YP772370BQE
CSB-EP772370BQE
CSB-BP772370BQE
CSB-MP772370BQE
CSB-EP772370BQE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant UPF0295 protein BA_0538/GBAA_0538/BAS0506 (BA_0538, GBAA_0538, BAS0506), partial CSB-YP772371BQE1
CSB-EP772371BQE1
CSB-BP772371BQE1
CSB-MP772371BQE1
CSB-EP772371BQE1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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