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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Tropheryma whipplei Protoheme IX farnesyltransferase (ctaB), partial CSB-YP772672TIW
CSB-EP772672TIW
CSB-BP772672TIW
CSB-MP772672TIW
CSB-EP772672TIW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Tropheryma whipplei Undecaprenyl pyrophosphate synthase 1 (uppS1) CSB-YP772673TIW
CSB-EP772673TIW
CSB-BP772673TIW
CSB-MP772673TIW
CSB-EP772673TIW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Tropheryma whipplei Ribonuclease H (rnhA) CSB-YP772674TIW
CSB-EP772674TIW
CSB-BP772674TIW
CSB-MP772674TIW
CSB-EP772674TIW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Tropheryma whipplei Dihydroxy-acid dehydratase (ilvD), partial CSB-YP772675TIW
CSB-EP772675TIW
CSB-BP772675TIW
CSB-MP772675TIW
CSB-EP772675TIW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Tropheryma whipplei 50S ribosomal protein L35 (rpmI) CSB-YP772676TIW
CSB-EP772676TIW
CSB-BP772676TIW
CSB-MP772676TIW
CSB-EP772676TIW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Tropheryma whipplei 50S ribosomal protein L33 (rpmG) CSB-YP772677TIW
CSB-EP772677TIW
CSB-BP772677TIW
CSB-MP772677TIW
CSB-EP772677TIW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Tropheryma whipplei 30S ribosomal protein S18 (rpsR) CSB-YP772678TIW
CSB-EP772678TIW
CSB-BP772678TIW
CSB-MP772678TIW
CSB-EP772678TIW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Tropheryma whipplei Pantothenate synthetase (panC) CSB-YP772679TIW
CSB-EP772679TIW
CSB-BP772679TIW
CSB-MP772679TIW
CSB-EP772679TIW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Tropheryma whipplei Probable histidine--tRNA ligase (hisS) CSB-YP772680TIV
CSB-EP772680TIV
CSB-BP772680TIV
CSB-MP772680TIV
CSB-EP772680TIV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Tropheryma whipplei Serine--tRNA ligase (serS) CSB-YP772681TIV
CSB-EP772681TIV
CSB-BP772681TIV
CSB-MP772681TIV
CSB-EP772681TIV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Tropheryma whipplei 6,7-dimethyl-8-ribityllumazine synthase (ribH) CSB-YP772682TIV
CSB-EP772682TIV
CSB-BP772682TIV
CSB-MP772682TIV
CSB-EP772682TIV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Tropheryma whipplei Exodeoxyribonuclease 7 large subunit (xseA) CSB-YP772683TIV
CSB-EP772683TIV
CSB-BP772683TIV
CSB-MP772683TIV
CSB-EP772683TIV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Tropheryma whipplei Translation initiation factor IF-2 (infB), partial CSB-YP772684TIV
CSB-EP772684TIV
CSB-BP772684TIV
CSB-MP772684TIV
CSB-EP772684TIV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Tropheryma whipplei Tyrosine--tRNA ligase (tyrS) CSB-YP772685TIV
CSB-EP772685TIV
CSB-BP772685TIV
CSB-MP772685TIV
CSB-EP772685TIV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Tropheryma whipplei Ribosomal RNA small subunit methyltransferase H (rsmH) CSB-YP772686TIV
CSB-EP772686TIV
CSB-BP772686TIV
CSB-MP772686TIV
CSB-EP772686TIV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Tropheryma whipplei Ribonuclease H (rnhA) CSB-YP772687TIV
CSB-EP772687TIV
CSB-BP772687TIV
CSB-MP772687TIV
CSB-EP772687TIV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Tropheryma whipplei Undecaprenyl pyrophosphate synthase 2 (uppS2) CSB-YP772688TIV
CSB-EP772688TIV
CSB-BP772688TIV
CSB-MP772688TIV
CSB-EP772688TIV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Tropheryma whipplei Putative cysteine--tRNA ligase 2 (cysS2) CSB-YP772689TIV
CSB-EP772689TIV
CSB-BP772689TIV
CSB-MP772689TIV
CSB-EP772689TIV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Tropheryma whipplei Guanylate kinase (gmk) CSB-YP772690TIV
CSB-EP772690TIV
CSB-BP772690TIV
CSB-MP772690TIV
CSB-EP772690TIV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Tropheryma whipplei Leucine--tRNA ligase (leuS), partial CSB-YP772691TIV
CSB-EP772691TIV
CSB-BP772691TIV
CSB-MP772691TIV
CSB-EP772691TIV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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