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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Probable crotonobetaine/carnitine-CoA ligase (caiC), partial CSB-YP774511SZB
CSB-EP774511SZB
CSB-BP774511SZB
CSB-MP774511SZB
CSB-EP774511SZB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Soluble pyridine nucleotide transhydrogenase (sthA) CSB-YP774512SZB
CSB-EP774512SZB
CSB-BP774512SZB
CSB-MP774512SZB
CSB-EP774512SZB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Isopentenyl-diphosphate Delta-isomerase (idi) CSB-YP774513SZB
CSB-EP774513SZB
CSB-BP774513SZB
CSB-MP774513SZB
CSB-EP774513SZB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant UDP-N-acetylglucosamine--N-acetylmuramyl- (pentapeptide) pyrophosphoryl-undecaprenol N-acetylglucosamine transferase (murG) CSB-YP774514SZB
CSB-EP774514SZB
CSB-BP774514SZB
CSB-MP774514SZB
CSB-EP774514SZB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Tropheryma whipplei DNA translocase FtsK (ftsK), partial CSB-YP774515TIW1
CSB-EP774515TIW1
CSB-BP774515TIW1
CSB-MP774515TIW1
CSB-EP774515TIW1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Tropheryma whipplei Putative sporulation transcription regulator WhiA (whiA) CSB-YP774516TIW
CSB-EP774516TIW
CSB-BP774516TIW
CSB-MP774516TIW
CSB-EP774516TIW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Tropheryma whipplei Holliday junction ATP-dependent DNA helicase RuvB (ruvB) CSB-YP774517TIW
CSB-EP774517TIW
CSB-BP774517TIW
CSB-MP774517TIW
CSB-EP774517TIW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Tropheryma whipplei Ribosome-binding factor A (rbfA) CSB-YP774518TIW
CSB-EP774518TIW
CSB-BP774518TIW
CSB-MP774518TIW
CSB-EP774518TIW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Tropheryma whipplei Protein grpE (grpE) CSB-YP774519TIV
CSB-EP774519TIV
CSB-BP774519TIV
CSB-MP774519TIV
CSB-EP774519TIV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Tropheryma whipplei SsrA-binding protein (smpB) CSB-YP774520TIV
CSB-EP774520TIV
CSB-BP774520TIV
CSB-MP774520TIV
CSB-EP774520TIV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Tropheryma whipplei UvrABC system protein B (uvrB), partial CSB-YP774521TIV
CSB-EP774521TIV
CSB-BP774521TIV
CSB-MP774521TIV
CSB-EP774521TIV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Tropheryma whipplei Lipoyl synthase (lipA) CSB-YP774522TIV
CSB-EP774522TIV
CSB-BP774522TIV
CSB-MP774522TIV
CSB-EP774522TIV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Tropheryma whipplei DNA replication and repair protein recF (recF) CSB-YP774523TIV
CSB-EP774523TIV
CSB-BP774523TIV
CSB-MP774523TIV
CSB-EP774523TIV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 4-hydroxy-2-oxo-heptane-1,7-dioate aldolase (hpcH) CSB-YP774524SZB
CSB-EP774524SZB
CSB-BP774524SZB
CSB-MP774524SZB
CSB-EP774524SZB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Fumarate reductase subunit C (frdC), partial CSB-YP774525SZB1
CSB-EP774525SZB1
CSB-BP774525SZB1
CSB-MP774525SZB1
CSB-EP774525SZB1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Maltose transport system permease protein malF (malF), partial CSB-YP774526SZB
CSB-EP774526SZB
CSB-BP774526SZB
CSB-MP774526SZB
CSB-EP774526SZB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Peptidase E (pepE) CSB-YP774527SZB
CSB-EP774527SZB
CSB-BP774527SZB
CSB-MP774527SZB
CSB-EP774527SZB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 50S ribosomal protein L1 (rplA) CSB-YP774528SZB
CSB-EP774528SZB
CSB-BP774528SZB
CSB-MP774528SZB
CSB-EP774528SZB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cation-efflux pump FieF (fieF), partial CSB-YP774529SZB1
CSB-EP774529SZB1
CSB-BP774529SZB1
CSB-MP774529SZB1
CSB-EP774529SZB1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Fatty acid oxidation complex subunit alpha (fadB), partial CSB-YP774530SZB
CSB-EP774530SZB
CSB-BP774530SZB
CSB-MP774530SZB
CSB-EP774530SZB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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