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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Nematostella vectensis Eukaryotic translation initiation factor 3 subunit L (v1g169424), partial CSB-YP007541NGO
CSB-EP007541NGO
CSB-BP007541NGO
CSB-MP007541NGO
CSB-EP007541NGO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nematostella vectensis Ribosomal RNA processing protein 36 homolog (v1g245966) CSB-YP004071NGO
CSB-EP004071NGO
CSB-BP004071NGO
CSB-MP004071NGO
CSB-EP004071NGO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nematostella vectensis Eukaryotic translation initiation factor 3 subunit D (v1g235689), partial CSB-YP007533NGO
CSB-EP007533NGO
CSB-BP007533NGO
CSB-MP007533NGO
CSB-EP007533NGO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nematostella vectensis Proteasome assembly chaperone 3 (psmg3) CSB-YP018923NGO
CSB-EP018923NGO
CSB-BP018923NGO
CSB-MP018923NGO
CSB-EP018923NGO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nematostella vectensis Pescadillo homolog (v1g194255), partial CSB-YP017791NGO
CSB-EP017791NGO
CSB-BP017791NGO
CSB-MP017791NGO
CSB-EP017791NGO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vanderwaltozyma polyspora Kynureninase (BNA5) CSB-YP012703VDW
CSB-EP012703VDW
CSB-BP012703VDW
CSB-MP012703VDW
CSB-EP012703VDW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vanderwaltozyma polyspora Autophagy protein 5 (ATG5) CSB-YP002293VDW
CSB-EP002293VDW
CSB-BP002293VDW
CSB-MP002293VDW
CSB-EP002293VDW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neurospora crassa U6 snRNA-associated Sm-like protein LSm6 (lsm-6) CSB-YP013209NHA
CSB-EP013209NHA
CSB-BP013209NHA
CSB-MP013209NHA
CSB-EP013209NHA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Glucose-6-phosphate isomerase (pgi) CSB-YP009717SUK
CSB-EP009717SUK
CSB-BP009717SUK
CSB-MP009717SUK
CSB-EP009717SUK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus ATP synthase subunit beta (atpD) CSB-YP002350SUK
CSB-EP002350SUK
CSB-BP002350SUK
CSB-MP002350SUK
CSB-EP002350SUK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pseudotuberculosis serotype O:1b Translation initiation factor IF-3 (infC) CSB-YP015166YAN
CSB-EP015166YAN
CSB-BP015166YAN
CSB-MP015166YAN
CSB-EP015166YAN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pseudotuberculosis serotype O:1b Galactokinase (galK) CSB-YP009198YAN
CSB-EP009198YAN
CSB-BP009198YAN
CSB-MP009198YAN
CSB-EP009198YAN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pseudotuberculosis serotype O:1b Lipoyl synthase (lipA) CSB-YP012927YAN
CSB-EP012927YAN
CSB-BP012927YAN
CSB-MP012927YAN
CSB-EP012927YAN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pseudotuberculosis serotype O:1b Queuine tRNA-ribosyltransferase (tgt) CSB-YP019146YAN
CSB-EP019146YAN
CSB-BP019146YAN
CSB-MP019146YAN
CSB-EP019146YAN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum Elongation factor Ts (tsf) CSB-YP025124CWU
CSB-EP025124CWU
CSB-BP025124CWU
CSB-MP025124CWU
CSB-EP025124CWU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum ATP synthase subunit b (atpF), partial CSB-YP002358CWU1
CSB-EP002358CWU1
CSB-BP002358CWU1
CSB-MP002358CWU1
CSB-EP002358CWU1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum Translation initiation factor IF-2 (infB), partial CSB-YP015164CWU
CSB-EP015164CWU
CSB-BP015164CWU
CSB-MP015164CWU
CSB-EP015164CWU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum S-adenosylmethionine decarboxylase proenzyme (speH) CSB-YP001658CWU
CSB-EP001658CWU
CSB-BP001658CWU
CSB-MP001658CWU
CSB-EP001658CWU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum Elongation factor Tu (tuf1) CSB-YP025342CWU
CSB-EP025342CWU
CSB-BP025342CWU
CSB-MP025342CWU
CSB-EP025342CWU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum Peptide chain release factor 1 (prfA) CSB-YP015197CWW
CSB-EP015197CWW
CSB-BP015197CWW
CSB-MP015197CWW
CSB-EP015197CWW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質形式,可以通過多種方式產(chǎn)生大量蛋白質、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質合成機制來產(chǎn)生由該基因編碼的蛋白質。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質?

根據(jù)重組蛋白的不同應用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務,您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質有任何疑問,涉及到蛋白質的價格、交貨、質量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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