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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Clostridium botulinum Peptide chain release factor 1 (prfA) CSB-YP015197CWU
CSB-EP015197CWU
CSB-BP015197CWU
CSB-MP015197CWU
CSB-EP015197CWU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum Glycine cleavage system H protein (gcvH) CSB-YP009335CWU
CSB-EP009335CWU
CSB-BP009335CWU
CSB-MP009335CWU
CSB-EP009335CWU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum Methylthioribose-1-phosphate isomerase (mtnA) CSB-YP014799CWU
CSB-EP014799CWU
CSB-BP014799CWU
CSB-MP014799CWU
CSB-EP014799CWU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum Ribosome-recycling factor (frr) CSB-YP014941CWW
CSB-EP014941CWW
CSB-BP014941CWW
CSB-MP014941CWW
CSB-EP014941CWW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus subsp. cytotoxis Thymidylate kinase (tmk) CSB-YP007226BOK
CSB-EP007226BOK
CSB-BP007226BOK
CSB-MP007226BOK
CSB-EP007226BOK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus subsp. cytotoxis Urocanate hydratase (hutU), partial CSB-YP025677BOK
CSB-EP025677BOK
CSB-BP025677BOK
CSB-MP025677BOK
CSB-EP025677BOK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus subsp. cytotoxis tRNA dimethylallyltransferase (miaA) CSB-YP024529BOK
CSB-EP024529BOK
CSB-BP024529BOK
CSB-MP024529BOK
CSB-EP024529BOK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus subsp. cytotoxis Malate dehydrogenase (mdh) CSB-YP013623BOK
CSB-EP013623BOK
CSB-BP013623BOK
CSB-MP013623BOK
CSB-EP013623BOK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus subsp. cytotoxis Lipoyl synthase (lipA) CSB-YP012927BOK
CSB-EP012927BOK
CSB-BP012927BOK
CSB-MP012927BOK
CSB-EP012927BOK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus subsp. cytotoxis Thymidine kinase (tdk) CSB-YP023577BOK
CSB-EP023577BOK
CSB-BP023577BOK
CSB-MP023577BOK
CSB-EP023577BOK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Campylobacter curvus ATP synthase subunit a (atpB), partial CSB-YP015070CWB1
CSB-EP015070CWB1
CSB-BP015070CWB1
CSB-MP015070CWB1
CSB-EP015070CWB1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Campylobacter jejuni subsp. doylei Queuine tRNA-ribosyltransferase (tgt) CSB-YP019146CWE
CSB-EP019146CWE
CSB-BP019146CWE
CSB-MP019146CWE
CSB-EP019146CWE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Campylobacter jejuni subsp. doylei Glucose-6-phosphate isomerase (pgi), partial CSB-YP009717CWE
CSB-EP009717CWE
CSB-BP009717CWE
CSB-MP009717CWE
CSB-EP009717CWE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Campylobacter jejuni subsp. doylei Porphobilinogen deaminase (hemC) CSB-YP010524CWE
CSB-EP010524CWE
CSB-BP010524CWE
CSB-MP010524CWE
CSB-EP010524CWE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Campylobacter jejuni subsp. doylei Ferrochelatase (hemH) CSB-YP008579CWE
CSB-EP008579CWE
CSB-BP008579CWE
CSB-MP008579CWE
CSB-EP008579CWE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Campylobacter jejuni subsp. doylei Acyl carrier protein (acpP) CSB-YP015636CWE
CSB-EP015636CWE
CSB-BP015636CWE
CSB-MP015636CWE
CSB-EP015636CWE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Anaeromyxobacter sp. Ribosome-recycling factor (frr) CSB-YP014941AUT
CSB-EP014941AUT
CSB-BP014941AUT
CSB-MP014941AUT
CSB-EP014941AUT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Anaeromyxobacter sp. Translation initiation factor IF-3 (infC) CSB-YP015166AUT
CSB-EP015166AUT
CSB-BP015166AUT
CSB-MP015166AUT
CSB-EP015166AUT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Anaeromyxobacter sp. Elongation factor Tu (tuf1) CSB-YP025342AUT
CSB-EP025342AUT
CSB-BP025342AUT
CSB-MP025342AUT
CSB-EP025342AUT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Anaeromyxobacter sp. ATP synthase subunit a (atpB), partial CSB-YP015070AUT1
CSB-EP015070AUT1
CSB-BP015070AUT1
CSB-MP015070AUT1
CSB-EP015070AUT1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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