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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Francisella tularensis subsp. holarctica Serine hydroxymethyltransferase (glyA) CSB-YP021274FDV
CSB-EP021274FDV
CSB-BP021274FDV
CSB-MP021274FDV
CSB-EP021274FDV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Francisella tularensis subsp. holarctica Triosephosphate isomerase (tpiA) CSB-YP024102FDV
CSB-EP024102FDV
CSB-BP024102FDV
CSB-MP024102FDV
CSB-EP024102FDV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Francisella tularensis subsp. holarctica ATP synthase subunit b (atpF), partial CSB-YP002358FDV1
CSB-EP002358FDV1
CSB-BP002358FDV1
CSB-MP002358FDV1
CSB-EP002358FDV1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Roseiflexus castenholzii Thymidine kinase (tdk) CSB-YP023577RIP
CSB-EP023577RIP
CSB-BP023577RIP
CSB-MP023577RIP
CSB-EP023577RIP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Roseiflexus castenholzii ATP synthase subunit b (atpF), partial CSB-YP002358RIP1
CSB-EP002358RIP1
CSB-BP002358RIP1
CSB-MP002358RIP1
CSB-EP002358RIP1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Roseiflexus castenholzii tRNA dimethylallyltransferase (miaA) CSB-YP024529RIP
CSB-EP024529RIP
CSB-BP024529RIP
CSB-MP024529RIP
CSB-EP024529RIP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nematostella vectensis Phosphotriesterase-related protein (v1g177083) CSB-YP018966NGO
CSB-EP018966NGO
CSB-BP018966NGO
CSB-MP018966NGO
CSB-EP018966NGO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nematostella vectensis Eukaryotic translation initiation factor 3 subunit J (v1g240395) CSB-YP007539NGO
CSB-EP007539NGO
CSB-BP007539NGO
CSB-MP007539NGO
CSB-EP007539NGO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nematostella vectensis Inosine triphosphate pyrophosphatase (v1g163483) CSB-YP011907NGO
CSB-EP011907NGO
CSB-BP011907NGO
CSB-MP011907NGO
CSB-EP011907NGO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nematostella vectensis Eukaryotic translation initiation factor 3 subunit A (v1g190699), partial CSB-YP007543NGO
CSB-EP007543NGO
CSB-BP007543NGO
CSB-MP007543NGO
CSB-EP007543NGO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nematostella vectensis Branched-chain-amino-acid aminotransferase (v1g246094) CSB-YP002601NGO
CSB-EP002601NGO
CSB-BP002601NGO
CSB-MP002601NGO
CSB-EP002601NGO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vanderwaltozyma polyspora Eukaryotic translation initiation factor 3 subunit G (TIF35) CSB-YP007536VDW
CSB-EP007536VDW
CSB-BP007536VDW
CSB-MP007536VDW
CSB-EP007536VDW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vanderwaltozyma polyspora Ubiquitin-like-conjugating enzyme ATG10 (ATG10) CSB-YP002282VDW
CSB-EP002282VDW
CSB-BP002282VDW
CSB-MP002282VDW
CSB-EP002282VDW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vanderwaltozyma polyspora Pescadillo homolog (NOP7), partial CSB-YP017791VDW
CSB-EP017791VDW
CSB-BP017791VDW
CSB-MP017791VDW
CSB-EP017791VDW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Thymidylate kinase (tmk) CSB-YP007226SUK
CSB-EP007226SUK
CSB-BP007226SUK
CSB-MP007226SUK
CSB-EP007226SUK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Elongation factor Tu (tuf) CSB-YP025342SUK
CSB-EP025342SUK
CSB-BP025342SUK
CSB-MP025342SUK
CSB-EP025342SUK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Triosephosphate isomerase (tpiA) CSB-YP024102SUK
CSB-EP024102SUK
CSB-BP024102SUK
CSB-MP024102SUK
CSB-EP024102SUK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Methionyl-tRNA formyltransferase (fmt) CSB-YP015150SUK
CSB-EP015150SUK
CSB-BP015150SUK
CSB-MP015150SUK
CSB-EP015150SUK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Acyl carrier protein (acpP) CSB-YP015636SUK
CSB-EP015636SUK
CSB-BP015636SUK
CSB-MP015636SUK
CSB-EP015636SUK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus GTPase Era (era) CSB-YP007759SUK
CSB-EP007759SUK
CSB-BP007759SUK
CSB-MP007759SUK
CSB-EP007759SUK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細(xì)胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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