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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Vibrio harveyi 6-phosphofructokinase (pfkA) CSB-YP017822VEA
CSB-EP017822VEA
CSB-BP017822VEA
CSB-MP017822VEA
CSB-EP017822VEA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio harveyi tRNA dimethylallyltransferase (miaA) CSB-YP024529VEA
CSB-EP024529VEA
CSB-BP024529VEA
CSB-MP024529VEA
CSB-EP024529VEA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio harveyi Nicotinate phosphoribosyltransferase (pncB) CSB-YP015451VEA
CSB-EP015451VEA
CSB-BP015451VEA
CSB-MP015451VEA
CSB-EP015451VEA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Francisella tularensis subsp. holarctica Porphobilinogen deaminase (hemC) CSB-YP010524FDV
CSB-EP010524FDV
CSB-BP010524FDV
CSB-MP010524FDV
CSB-EP010524FDV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Francisella tularensis subsp. holarctica Protoheme IX farnesyltransferase (cyoE), partial CSB-YP005824FDV
CSB-EP005824FDV
CSB-BP005824FDV
CSB-MP005824FDV
CSB-EP005824FDV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Francisella tularensis subsp. holarctica Thymidylate synthase (thyA) CSB-YP025393FDV
CSB-EP025393FDV
CSB-BP025393FDV
CSB-MP025393FDV
CSB-EP025393FDV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Francisella tularensis subsp. holarctica GMP synthase [glutamine-hydrolyzing] (guaA), partial CSB-YP009582FDV
CSB-EP009582FDV
CSB-BP009582FDV
CSB-MP009582FDV
CSB-EP009582FDV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Francisella tularensis subsp. holarctica ATP synthase subunit delta (atpH) CSB-YP002355FDV
CSB-EP002355FDV
CSB-BP002355FDV
CSB-MP002355FDV
CSB-EP002355FDV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nematostella vectensis Proteasome assembly chaperone 1 (psmg1) CSB-YP018921NGO
CSB-EP018921NGO
CSB-BP018921NGO
CSB-MP018921NGO
CSB-EP018921NGO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nematostella vectensis THO complex subunit 7 homolog (thoc7) CSB-YP023507NGO
CSB-EP023507NGO
CSB-BP023507NGO
CSB-MP023507NGO
CSB-EP023507NGO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nematostella vectensis U1 small nuclear ribonucleoprotein C (v1g244745) CSB-YP022331NGO
CSB-EP022331NGO
CSB-BP022331NGO
CSB-MP022331NGO
CSB-EP022331NGO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nematostella vectensis Proteasome assembly chaperone 2 (psmg2) CSB-YP018922NGO
CSB-EP018922NGO
CSB-BP018922NGO
CSB-MP018922NGO
CSB-EP018922NGO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nematostella vectensis Eukaryotic translation initiation factor 3 subunit M (v1g192238) CSB-YP007542NGO
CSB-EP007542NGO
CSB-BP007542NGO
CSB-MP007542NGO
CSB-EP007542NGO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vanderwaltozyma polyspora Exportin-T (LOS1), partial CSB-YP026226VDW
CSB-EP026226VDW
CSB-BP026226VDW
CSB-MP026226VDW
CSB-EP026226VDW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vanderwaltozyma polyspora Ubiquitin-like modifier-activating enzyme ATG7 (ATG7), partial CSB-YP002294VDW
CSB-EP002294VDW
CSB-BP002294VDW
CSB-MP002294VDW
CSB-EP002294VDW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vanderwaltozyma polyspora Flap endonuclease 1 (FEN1) CSB-YP008585VDW
CSB-EP008585VDW
CSB-BP008585VDW
CSB-MP008585VDW
CSB-EP008585VDW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vanderwaltozyma polyspora Ubiquitin-like protein ATG12 (ATG12) CSB-YP002283VDW
CSB-EP002283VDW
CSB-BP002283VDW
CSB-MP002283VDW
CSB-EP002283VDW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vanderwaltozyma polyspora Eukaryotic translation initiation factor 3 subunit A (TIF32), partial CSB-YP007543VDW
CSB-EP007543VDW
CSB-BP007543VDW
CSB-MP007543VDW
CSB-EP007543VDW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vanderwaltozyma polyspora Enolase-phosphatase E1 (UTR4) CSB-YP007674VDW
CSB-EP007674VDW
CSB-BP007674VDW
CSB-MP007674VDW
CSB-EP007674VDW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vanderwaltozyma polyspora NADH-cytochrome b5 reductase 1 (CBR1), partial CSB-YP006318VDW1
CSB-EP006318VDW1
CSB-BP006318VDW1
CSB-MP006318VDW1
CSB-EP006318VDW1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務,您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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