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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Parvibaculum lavamentivorans Thymidylate synthase (thyA) CSB-YP025393PYE
CSB-EP025393PYE
CSB-BP025393PYE
CSB-MP025393PYE
CSB-EP025393PYE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xanthobacter autotrophicus tRNA dimethylallyltransferase (miaA) CSB-YP024529XAV
CSB-EP024529XAV
CSB-BP024529XAV
CSB-MP024529XAV
CSB-EP024529XAV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xanthobacter autotrophicus Glycine cleavage system H protein (gcvH) CSB-YP009335XAV
CSB-EP009335XAV
CSB-BP009335XAV
CSB-MP009335XAV
CSB-EP009335XAV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xanthobacter autotrophicus Nucleoside diphosphate kinase (ndk) CSB-YP015889XAV
CSB-EP015889XAV
CSB-BP015889XAV
CSB-MP015889XAV
CSB-EP015889XAV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xanthobacter autotrophicus Lipoyl synthase (lipA) CSB-YP012927XAV
CSB-EP012927XAV
CSB-BP012927XAV
CSB-MP012927XAV
CSB-EP012927XAV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pichia angusta Ubiquitin-like-conjugating enzyme ATG10 (ATG10) CSB-YP002282EVQ
CSB-EP002282EVQ
CSB-BP002282EVQ
CSB-MP002282EVQ
CSB-EP002282EVQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Artemia franciscana 60S ribosomal protein L38 (RPL38) CSB-YP020275DOF
CSB-EP020275DOF
CSB-BP020275DOF
CSB-MP020275DOF
CSB-EP020275DOF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cronobacter sakazakii Glycogen debranching enzyme (glgX), partial CSB-YP001446CXL
CSB-EP001446CXL
CSB-BP001446CXL
CSB-MP001446CXL
CSB-EP001446CXL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cronobacter sakazakii GMP synthase [glutamine-hydrolyzing] (guaA), partial CSB-YP009582CXL
CSB-EP009582CXL
CSB-BP009582CXL
CSB-MP009582CXL
CSB-EP009582CXL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cronobacter sakazakii L-threonine 3-dehydrogenase (tdh) CSB-YP023350CXL
CSB-EP023350CXL
CSB-BP023350CXL
CSB-MP023350CXL
CSB-EP023350CXL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cronobacter sakazakii ATP synthase subunit delta (atpH) CSB-YP002355CXL
CSB-EP002355CXL
CSB-BP002355CXL
CSB-MP002355CXL
CSB-EP002355CXL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cronobacter sakazakii Cardiolipin synthase (cls), partial CSB-YP005985CXL1
CSB-EP005985CXL1
CSB-BP005985CXL1
CSB-MP005985CXL1
CSB-EP005985CXL1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cronobacter sakazakii Malate dehydrogenase (mdh) CSB-YP013623CXL
CSB-EP013623CXL
CSB-BP013623CXL
CSB-MP013623CXL
CSB-EP013623CXL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cronobacter sakazakii Porphobilinogen deaminase (hemC) CSB-YP010524CXL
CSB-EP010524CXL
CSB-BP010524CXL
CSB-MP010524CXL
CSB-EP010524CXL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cronobacter sakazakii Glycine cleavage system H protein (gcvH) CSB-YP009335CXL
CSB-EP009335CXL
CSB-BP009335CXL
CSB-MP009335CXL
CSB-EP009335CXL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio harveyi Queuine tRNA-ribosyltransferase (tgt) CSB-YP019146VEA
CSB-EP019146VEA
CSB-BP019146VEA
CSB-MP019146VEA
CSB-EP019146VEA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio harveyi Cysteine desulfurase (iscS) CSB-YP015769VEA
CSB-EP015769VEA
CSB-BP015769VEA
CSB-MP015769VEA
CSB-EP015769VEA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio harveyi Urocanate hydratase (hutU), partial CSB-YP025677VEA
CSB-EP025677VEA
CSB-BP025677VEA
CSB-MP025677VEA
CSB-EP025677VEA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio harveyi Oligoribonuclease (orn) CSB-YP019586VEA
CSB-EP019586VEA
CSB-BP019586VEA
CSB-MP019586VEA
CSB-EP019586VEA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Francisella tularensis subsp. holarctica Nucleoside diphosphate kinase (ndk) CSB-YP015889FDV
CSB-EP015889FDV
CSB-BP015889FDV
CSB-MP015889FDV
CSB-EP015889FDV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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