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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Malassezia globosa Pescadillo homolog (NOP7), partial CSB-YP017791MNH
CSB-EP017791MNH
CSB-BP017791MNH
CSB-MP017791MNH
CSB-EP017791MNH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brugia malayi Lateral signaling target protein 2 homolog (Bm1_49520), partial CSB-YP026476BWV
CSB-EP026476BWV
CSB-BP026476BWV
CSB-MP026476BWV
CSB-EP026476BWV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bungarus multicinctus L-amino-acid oxidase CSB-YP011660BXN
CSB-EP011660BXN
CSB-BP011660BXN
CSB-MP011660BXN
CSB-EP011660BXN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Danio rerio Leucine-rich repeat-containing protein 3 (lrrc3), partial CSB-YP013124DIL1
CSB-EP013124DIL1
CSB-BP013124DIL1
CSB-MP013124DIL1
CSB-EP013124DIL1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Adenylyltransferase and sulfurtransferase MOCS3 (CBG01549) CSB-YP014707CXX
CSB-EP014707CXX
CSB-BP014707CXX
CSB-MP014707CXX
CSB-EP014707CXX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Protein PAT1 homolog 1 (patr-1), partial CSB-YP017483CXX
CSB-EP017483CXX
CSB-BP017483CXX
CSB-MP017483CXX
CSB-EP017483CXX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ribosome biogenesis regulatory protein homolog (rrbs-1) CSB-YP020533CXX
CSB-EP020533CXX
CSB-BP020533CXX
CSB-MP020533CXX
CSB-EP020533CXX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pescadillo homolog (lpd-7), partial CSB-YP017791CXX
CSB-EP017791CXX
CSB-BP017791CXX
CSB-MP017791CXX
CSB-EP017791CXX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Probable polyprenol reductase (CBG09584), partial CSB-YP022655CXX1
CSB-EP022655CXX1
CSB-BP022655CXX1
CSB-MP022655CXX1
CSB-EP022655CXX1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ubiquitin-fold modifier-conjugating enzyme 1 (CBG10119) CSB-YP025558CXX
CSB-EP025558CXX
CSB-BP025558CXX
CSB-MP025558CXX
CSB-EP025558CXX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ubiquitin-like modifier-activating enzyme 5 (CBG11977) CSB-YP025415CXX
CSB-EP025415CXX
CSB-BP025415CXX
CSB-MP025415CXX
CSB-EP025415CXX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Protein SEC13 homolog (npp-20) CSB-YP020934CXX
CSB-EP020934CXX
CSB-BP020934CXX
CSB-MP020934CXX
CSB-EP020934CXX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus helveticus tRNA pseudouridine synthase A (truA) CSB-YP019086LLE
CSB-EP019086LLE
CSB-BP019086LLE
CSB-MP019086LLE
CSB-EP019086LLE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus helveticus ATP synthase subunit delta (atpH) CSB-YP002355LLE
CSB-EP002355LLE
CSB-BP002355LLE
CSB-MP002355LLE
CSB-EP002355LLE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus helveticus Thymidylate synthase (thyA) CSB-YP025393LLE
CSB-EP025393LLE
CSB-BP025393LLE
CSB-MP025393LLE
CSB-EP025393LLE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus helveticus Phosphoserine aminotransferase (serC) CSB-YP018838LLE
CSB-EP018838LLE
CSB-BP018838LLE
CSB-MP018838LLE
CSB-EP018838LLE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus ATP synthase subunit beta (atpD) CSB-YP002350SUM
CSB-EP002350SUM
CSB-BP002350SUM
CSB-MP002350SUM
CSB-EP002350SUM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus ATP synthase subunit delta (atpH) CSB-YP002355SUM
CSB-EP002355SUM
CSB-BP002355SUM
CSB-MP002355SUM
CSB-EP002355SUM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Peptide chain release factor 1 (prfA) CSB-YP015197SUM
CSB-EP015197SUM
CSB-BP015197SUM
CSB-MP015197SUM
CSB-EP015197SUM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Triosephosphate isomerase (tpiA) CSB-YP024102SUM
CSB-EP024102SUM
CSB-BP024102SUM
CSB-MP024102SUM
CSB-EP024102SUM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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