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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant tRNA (mo5U34)-methyltransferase (cmoB) CSB-YP803315VFE
CSB-EP803315VFE
CSB-BP803315VFE
CSB-MP803315VFE
CSB-EP803315VFE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Glucose-1-phosphate adenylyltransferase 1 (glgC1) CSB-YP803316VFE
CSB-EP803316VFE
CSB-BP803316VFE
CSB-MP803316VFE
CSB-EP803316VFE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant UPF0319 protein VP1009 (VP1009) CSB-YP803317VFE
CSB-EP803317VFE
CSB-BP803317VFE
CSB-MP803317VFE
CSB-EP803317VFE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant ATP-dependent Clp protease ATP-binding subunit ClpX (clpX) CSB-YP803318VFE
CSB-EP803318VFE
CSB-BP803318VFE
CSB-MP803318VFE
CSB-EP803318VFE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Succinyl-CoA ligase [ADP-forming] subunit beta (sucC) CSB-YP803319VFE
CSB-EP803319VFE
CSB-BP803319VFE
CSB-MP803319VFE
CSB-EP803319VFE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cell division protein ZipA homolog (zipA), partial CSB-YP803320VFE
CSB-EP803320VFE
CSB-BP803320VFE
CSB-MP803320VFE
CSB-EP803320VFE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Glutamyl-tRNA reductase (hemA) CSB-YP803321VFE
CSB-EP803321VFE
CSB-BP803321VFE
CSB-MP803321VFE
CSB-EP803321VFE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant UPF0250 protein VP0718 (VP0718) CSB-YP803322VFE
CSB-EP803322VFE
CSB-BP803322VFE
CSB-MP803322VFE
CSB-EP803322VFE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Protein syd (syd) CSB-YP803323VFE
CSB-EP803323VFE
CSB-BP803323VFE
CSB-MP803323VFE
CSB-EP803323VFE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Exodeoxyribonuclease 7 small subunit (xseB) CSB-YP803324VFE
CSB-EP803324VFE
CSB-BP803324VFE
CSB-MP803324VFE
CSB-EP803324VFE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Transcriptional repressor NrdR (nrdR) CSB-YP803325VFE
CSB-EP803325VFE
CSB-BP803325VFE
CSB-MP803325VFE
CSB-EP803325VFE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant GTPase Der (der) CSB-YP803326VFE
CSB-EP803326VFE
CSB-BP803326VFE
CSB-MP803326VFE
CSB-EP803326VFE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Queuine tRNA-ribosyltransferase (tgt) CSB-YP803327VFE
CSB-EP803327VFE
CSB-BP803327VFE
CSB-MP803327VFE
CSB-EP803327VFE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ribosomal large subunit pseudouridine synthase D (rluD) CSB-YP803328VFE
CSB-EP803328VFE
CSB-BP803328VFE
CSB-MP803328VFE
CSB-EP803328VFE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Uncharacterized response regulatory protein VP0538 (VP0538) CSB-YP803329VFE
CSB-EP803329VFE
CSB-BP803329VFE
CSB-MP803329VFE
CSB-EP803329VFE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prolipoprotein diacylglyceryl transferase (lgt), partial CSB-YP803330VFE1
CSB-EP803330VFE1
CSB-BP803330VFE1
CSB-MP803330VFE1
CSB-EP803330VFE1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Homoserine kinase (thrB) CSB-YP803331VFE
CSB-EP803331VFE
CSB-BP803331VFE
CSB-MP803331VFE
CSB-EP803331VFE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dihydrodipicolinate reductase (dapB) CSB-YP803332VFE
CSB-EP803332VFE
CSB-BP803332VFE
CSB-MP803332VFE
CSB-EP803332VFE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 50S ribosomal protein L13 (rplM) CSB-YP803333VFE
CSB-EP803333VFE
CSB-BP803333VFE
CSB-MP803333VFE
CSB-EP803333VFE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mannitol-1-phosphate 5-dehydrogenase (mtlD) CSB-YP803334VFE
CSB-EP803334VFE
CSB-BP803334VFE
CSB-MP803334VFE
CSB-EP803334VFE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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