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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Buchnera aphidicola subsp. Baizongia pistaciae Glutamate--cysteine ligase (gshA), partial CSB-YP804144BMZ
CSB-EP804144BMZ
CSB-BP804144BMZ
CSB-MP804144BMZ
CSB-EP804144BMZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Buchnera aphidicola subsp. Baizongia pistaciae Signal recognition particle protein (ffh) CSB-YP804145BMZ
CSB-EP804145BMZ
CSB-BP804145BMZ
CSB-MP804145BMZ
CSB-EP804145BMZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Buchnera aphidicola subsp. Baizongia pistaciae Transcription elongation protein nusA (nusA) CSB-YP804146BMZ
CSB-EP804146BMZ
CSB-BP804146BMZ
CSB-MP804146BMZ
CSB-EP804146BMZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Buchnera aphidicola subsp. Baizongia pistaciae PTS system glucose-specific EIICB component (ptsG), partial CSB-YP804147BMZ
CSB-EP804147BMZ
CSB-BP804147BMZ
CSB-MP804147BMZ
CSB-EP804147BMZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Buchnera aphidicola subsp. Baizongia pistaciae Putative flagellar protein flgJ homolog (flgJ) CSB-YP804148BMZ
CSB-EP804148BMZ
CSB-BP804148BMZ
CSB-MP804148BMZ
CSB-EP804148BMZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Buchnera aphidicola subsp. Baizongia pistaciae Flagellar basal body rod protein FlgB (flgB) CSB-YP804149BMZ
CSB-EP804149BMZ
CSB-BP804149BMZ
CSB-MP804149BMZ
CSB-EP804149BMZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Buchnera aphidicola subsp. Baizongia pistaciae Glucose-6-phosphate 1-dehydrogenase (zwf) CSB-YP804150BMZ
CSB-EP804150BMZ
CSB-BP804150BMZ
CSB-MP804150BMZ
CSB-EP804150BMZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Buchnera aphidicola subsp. Baizongia pistaciae 2,3-bisphosphoglycerate-dependent phosphoglycerate mutase (gpmA) CSB-YP804151BMZ
CSB-EP804151BMZ
CSB-BP804151BMZ
CSB-MP804151BMZ
CSB-EP804151BMZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Buchnera aphidicola subsp. Baizongia pistaciae Biotin synthase (bioB) CSB-YP804152BMZ
CSB-EP804152BMZ
CSB-BP804152BMZ
CSB-MP804152BMZ
CSB-EP804152BMZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Buchnera aphidicola subsp. Baizongia pistaciae Putative protease sohB (sohB), partial CSB-YP804153BMZ1
CSB-EP804153BMZ1
CSB-BP804153BMZ1
CSB-MP804153BMZ1
CSB-EP804153BMZ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Buchnera aphidicola subsp. Baizongia pistaciae UPF0056 membrane protein bbp_248 (bbp_248), partial CSB-YP804154BMZ1
CSB-EP804154BMZ1
CSB-BP804154BMZ1
CSB-MP804154BMZ1
CSB-EP804154BMZ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Buchnera aphidicola subsp. Baizongia pistaciae Signal peptidase I (lepB), partial CSB-YP804155BMZ
CSB-EP804155BMZ
CSB-BP804155BMZ
CSB-MP804155BMZ
CSB-EP804155BMZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Buchnera aphidicola subsp. Baizongia pistaciae Flagellar biosynthesis protein flhA (flhA), partial CSB-YP804156BMZ
CSB-EP804156BMZ
CSB-BP804156BMZ
CSB-MP804156BMZ
CSB-EP804156BMZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Buchnera aphidicola subsp. Baizongia pistaciae Methionine aminopeptidase (map) CSB-YP804157BMZ
CSB-EP804157BMZ
CSB-BP804157BMZ
CSB-MP804157BMZ
CSB-EP804157BMZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Buchnera aphidicola subsp. Baizongia pistaciae Acetolactate synthase large subunit (ilvI), partial CSB-YP804158BMZ
CSB-EP804158BMZ
CSB-BP804158BMZ
CSB-MP804158BMZ
CSB-EP804158BMZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Buchnera aphidicola subsp. Baizongia pistaciae Lipid II flippase FtsW (ftsW), partial CSB-YP804159BMZ
CSB-EP804159BMZ
CSB-BP804159BMZ
CSB-MP804159BMZ
CSB-EP804159BMZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Buchnera aphidicola subsp. Baizongia pistaciae GTP-dependent nucleic acid-binding protein engD (engD) CSB-YP804160BMZ
CSB-EP804160BMZ
CSB-BP804160BMZ
CSB-MP804160BMZ
CSB-EP804160BMZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Buchnera aphidicola subsp. Baizongia pistaciae Probable peroxiredoxin (bbp_171) CSB-YP804161BMZ
CSB-EP804161BMZ
CSB-BP804161BMZ
CSB-MP804161BMZ
CSB-EP804161BMZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Buchnera aphidicola subsp. Baizongia pistaciae Colicin V production protein homolog (cvpA), partial CSB-YP804162BMZ1
CSB-EP804162BMZ1
CSB-BP804162BMZ1
CSB-MP804162BMZ1
CSB-EP804162BMZ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Buchnera aphidicola subsp. Baizongia pistaciae NADH-quinone oxidoreductase subunit I (nuoI) CSB-YP804163BMZ
CSB-EP804163BMZ
CSB-BP804163BMZ
CSB-MP804163BMZ
CSB-EP804163BMZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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