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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Alkaliphilus oremlandii Elongation factor Ts (tsf) CSB-YP025124AUQ
CSB-EP025124AUQ
CSB-BP025124AUQ
CSB-MP025124AUQ
CSB-EP025124AUQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Alkaliphilus oremlandii Phosphatidylserine decarboxylase proenzyme (psd) CSB-YP018033AUQ
CSB-EP018033AUQ
CSB-BP018033AUQ
CSB-MP018033AUQ
CSB-EP018033AUQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Alkaliphilus oremlandii ATP synthase subunit alpha (atpA), partial CSB-YP002344AUQ
CSB-EP002344AUQ
CSB-BP002344AUQ
CSB-MP002344AUQ
CSB-EP002344AUQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Alkaliphilus oremlandii Elongation factor G (fusA), partial CSB-YP009373AUQ
CSB-EP009373AUQ
CSB-BP009373AUQ
CSB-MP009373AUQ
CSB-EP009373AUQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Alkaliphilus oremlandii S-adenosylmethionine decarboxylase proenzyme (speD) CSB-YP001658AUQ
CSB-EP001658AUQ
CSB-BP001658AUQ
CSB-MP001658AUQ
CSB-EP001658AUQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Caldivirga maquilingensis Serine hydroxymethyltransferase (glyA) CSB-YP021274CVY
CSB-EP021274CVY
CSB-BP021274CVY
CSB-MP021274CVY
CSB-EP021274CVY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Alkaliphilus oremlandii Serine hydroxymethyltransferase (glyA) CSB-YP021274AUQ
CSB-EP021274AUQ
CSB-BP021274AUQ
CSB-MP021274AUQ
CSB-EP021274AUQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Alkaliphilus oremlandii Methionyl-tRNA formyltransferase (fmt) CSB-YP015150AUQ
CSB-EP015150AUQ
CSB-BP015150AUQ
CSB-MP015150AUQ
CSB-EP015150AUQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Coprinopsis cinerea Acetyl-coenzyme A synthetase (ACS-1), partial CSB-YP001203DWQ
CSB-EP001203DWQ
CSB-BP001203DWQ
CSB-MP001203DWQ
CSB-EP001203DWQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Malassezia globosa Structure-specific endonuclease subunit SLX1 (SLX1) CSB-YP009442MNH
CSB-EP009442MNH
CSB-BP009442MNH
CSB-MP009442MNH
CSB-EP009442MNH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brugia malayi Eukaryotic translation initiation factor 3 subunit I (Bm1_48300) CSB-YP007538BWV
CSB-EP007538BWV
CSB-BP007538BWV
CSB-MP007538BWV
CSB-EP007538BWV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brugia malayi Lysine-specific demethylase NO66 (Bm1_53875), partial CSB-YP003168BWV
CSB-EP003168BWV
CSB-BP003168BWV
CSB-MP003168BWV
CSB-EP003168BWV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Naja atra L-amino-acid oxidase CSB-YP011660NFG
CSB-EP011660NFG
CSB-BP011660NFG
CSB-MP011660NFG
CSB-EP011660NFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Trichosurus vulpecula CD302 antigen (CD302), partial CSB-YP004923TQG1
CSB-EP004923TQG1
CSB-BP004923TQG1
CSB-MP004923TQG1
CSB-EP004923TQG1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Probable methylthioribulose-1-phosphate dehydratase (CBG01968) CSB-YP001906CXX
CSB-EP001906CXX
CSB-BP001906CXX
CSB-MP001906CXX
CSB-EP001906CXX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Eukaryotic translation initiation factor 3 subunit H (eif-3.H) CSB-YP007537CXX
CSB-EP007537CXX
CSB-BP007537CXX
CSB-MP007537CXX
CSB-EP007537CXX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lysophospholipid acyltransferase 7 (mboa-7), partial CSB-YP013550CXX
CSB-EP013550CXX
CSB-BP013550CXX
CSB-MP013550CXX
CSB-EP013550CXX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Tyrosine 3-monooxygenase (cat-2), partial CSB-YP023470CXX
CSB-EP023470CXX
CSB-BP023470CXX
CSB-MP023470CXX
CSB-EP023470CXX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Superoxide dismutase [Cu-Zn] (sod-1) CSB-YP022397CXX
CSB-EP022397CXX
CSB-BP022397CXX
CSB-MP022397CXX
CSB-EP022397CXX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enolase-phosphatase E1 (CBG23332) CSB-YP007674CXX
CSB-EP007674CXX
CSB-BP007674CXX
CSB-MP007674CXX
CSB-EP007674CXX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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