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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Bacteroides thetaiotaomicron 50S ribosomal protein L7/L12 (rplL) CSB-YP805181BDU
CSB-EP805181BDU
CSB-BP805181BDU
CSB-MP805181BDU
CSB-EP805181BDU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacteroides thetaiotaomicron 50S ribosomal protein L4 (rplD) CSB-YP805182BDU
CSB-EP805182BDU
CSB-BP805182BDU
CSB-MP805182BDU
CSB-EP805182BDU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacteroides thetaiotaomicron 30S ribosomal protein S17 1 (rpsQ1) CSB-YP805183BDU
CSB-EP805183BDU
CSB-BP805183BDU
CSB-MP805183BDU
CSB-EP805183BDU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacteroides thetaiotaomicron 30S ribosomal protein S5 (rpsE) CSB-YP805184BDU
CSB-EP805184BDU
CSB-BP805184BDU
CSB-MP805184BDU
CSB-EP805184BDU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacteroides thetaiotaomicron Imidazolonepropionase (hutI) CSB-YP805185BDU
CSB-EP805185BDU
CSB-BP805185BDU
CSB-MP805185BDU
CSB-EP805185BDU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacteroides thetaiotaomicron Potassium-transporting ATPase A chain (kdpA), partial CSB-YP805186BDU
CSB-EP805186BDU
CSB-BP805186BDU
CSB-MP805186BDU
CSB-EP805186BDU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacteroides thetaiotaomicron Ribosome-recycling factor (frr) CSB-YP805187BDU
CSB-EP805187BDU
CSB-BP805187BDU
CSB-MP805187BDU
CSB-EP805187BDU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacteroides thetaiotaomicron Aspartate--ammonia ligase (asnA) CSB-YP805188BDU
CSB-EP805188BDU
CSB-BP805188BDU
CSB-MP805188BDU
CSB-EP805188BDU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacteroides thetaiotaomicron 6-phosphofructokinase 2 (pfkA2) CSB-YP805189BDU
CSB-EP805189BDU
CSB-BP805189BDU
CSB-MP805189BDU
CSB-EP805189BDU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacteroides thetaiotaomicron Holliday junction ATP-dependent DNA helicase RuvA (ruvA) CSB-YP805190BDU
CSB-EP805190BDU
CSB-BP805190BDU
CSB-MP805190BDU
CSB-EP805190BDU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacteroides thetaiotaomicron Histidine--tRNA ligase (hisS) CSB-YP805191BDU
CSB-EP805191BDU
CSB-BP805191BDU
CSB-MP805191BDU
CSB-EP805191BDU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacteroides thetaiotaomicron 7-cyano-7-deazaguanine synthase (queC) CSB-YP805192BDU
CSB-EP805192BDU
CSB-BP805192BDU
CSB-MP805192BDU
CSB-EP805192BDU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacteroides thetaiotaomicron Imidazole glycerol phosphate synthase subunit HisH (hisH) CSB-YP805193BDU
CSB-EP805193BDU
CSB-BP805193BDU
CSB-MP805193BDU
CSB-EP805193BDU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacteroides thetaiotaomicron Spermidine/putrescine import ATP-binding protein PotA (potA) CSB-YP805194BDU
CSB-EP805194BDU
CSB-BP805194BDU
CSB-MP805194BDU
CSB-EP805194BDU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacteroides thetaiotaomicron Putative ribosome biogenesis GTPase RsgA 1 (rsgA1) CSB-YP805195BDU
CSB-EP805195BDU
CSB-BP805195BDU
CSB-MP805195BDU
CSB-EP805195BDU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacteroides thetaiotaomicron Recombination protein RecR (recR) CSB-YP805196BDU
CSB-EP805196BDU
CSB-BP805196BDU
CSB-MP805196BDU
CSB-EP805196BDU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacteroides thetaiotaomicron 50S ribosomal protein L33 (rpmG) CSB-YP805197BDU
CSB-EP805197BDU
CSB-BP805197BDU
CSB-MP805197BDU
CSB-EP805197BDU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacteroides thetaiotaomicron tRNA1 (Val) (adenine (37)-N6)-methyltransferase (BT_0838) CSB-YP805198BDU
CSB-EP805198BDU
CSB-BP805198BDU
CSB-MP805198BDU
CSB-EP805198BDU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacteroides thetaiotaomicron Formate--tetrahydrofolate ligase (fhs), partial CSB-YP805199BDU
CSB-EP805199BDU
CSB-BP805199BDU
CSB-MP805199BDU
CSB-EP805199BDU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacteroides thetaiotaomicron ATP synthase subunit beta (atpD), partial CSB-YP805200BDU
CSB-EP805200BDU
CSB-BP805200BDU
CSB-MP805200BDU
CSB-EP805200BDU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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