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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Buchnera aphidicola subsp. Baizongia pistaciae 30S ribosomal protein S20 (rpsT) CSB-YP804164BMZ
CSB-EP804164BMZ
CSB-BP804164BMZ
CSB-MP804164BMZ
CSB-EP804164BMZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Buchnera aphidicola subsp. Baizongia pistaciae 50S ribosomal protein L25 (rplY) CSB-YP804165BMZ
CSB-EP804165BMZ
CSB-BP804165BMZ
CSB-MP804165BMZ
CSB-EP804165BMZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Buchnera aphidicola subsp. Baizongia pistaciae Phospho-2-dehydro-3-deoxyheptonate aldolase, Trp-sensitive (aroH) CSB-YP804166BMZ
CSB-EP804166BMZ
CSB-BP804166BMZ
CSB-MP804166BMZ
CSB-EP804166BMZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Buchnera aphidicola subsp. Baizongia pistaciae Electron transport complex protein RnfD (rnfD), partial CSB-YP804167BMZ1
CSB-EP804167BMZ1
CSB-BP804167BMZ1
CSB-MP804167BMZ1
CSB-EP804167BMZ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Buchnera aphidicola subsp. Baizongia pistaciae 6-phosphogluconate dehydrogenase, decarboxylating (gnd) CSB-YP804168BMZ
CSB-EP804168BMZ
CSB-BP804168BMZ
CSB-MP804168BMZ
CSB-EP804168BMZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Buchnera aphidicola subsp. Baizongia pistaciae Transaldolase (tal) CSB-YP804169BMZ
CSB-EP804169BMZ
CSB-BP804169BMZ
CSB-MP804169BMZ
CSB-EP804169BMZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Buchnera aphidicola subsp. Baizongia pistaciae Flagellar biosynthetic protein fliR (fliR), partial CSB-YP804170BMZ1
CSB-EP804170BMZ1
CSB-BP804170BMZ1
CSB-MP804170BMZ1
CSB-EP804170BMZ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Buchnera aphidicola subsp. Baizongia pistaciae Flagellum-specific ATP synthase (fliI) CSB-YP804171BMZ
CSB-EP804171BMZ
CSB-BP804171BMZ
CSB-MP804171BMZ
CSB-EP804171BMZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Buchnera aphidicola subsp. Baizongia pistaciae Glucose-specific phosphotransferase enzyme IIA component (crr) CSB-YP804172BMZ
CSB-EP804172BMZ
CSB-BP804172BMZ
CSB-MP804172BMZ
CSB-EP804172BMZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Buchnera aphidicola subsp. Baizongia pistaciae 5,10-methylenetetrahydrofolate reductase (metF) CSB-YP804173BMZ
CSB-EP804173BMZ
CSB-BP804173BMZ
CSB-MP804173BMZ
CSB-EP804173BMZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Buchnera aphidicola subsp. Baizongia pistaciae Uncharacterized protein bbp_030 (bbp_030) CSB-YP804174BMZ
CSB-EP804174BMZ
CSB-BP804174BMZ
CSB-MP804174BMZ
CSB-EP804174BMZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Buchnera aphidicola subsp. Baizongia pistaciae Uncharacterized KamA family protein bbp_022 (bbp_022) CSB-YP804175BMZ
CSB-EP804175BMZ
CSB-BP804175BMZ
CSB-MP804175BMZ
CSB-EP804175BMZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Buchnera aphidicola subsp. Baizongia pistaciae ATP synthase subunit delta (atpH) CSB-YP804176BMZ
CSB-EP804176BMZ
CSB-BP804176BMZ
CSB-MP804176BMZ
CSB-EP804176BMZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium japonicum Orotate phosphoribosyltransferase (pyrE) CSB-YP804177BVW
CSB-EP804177BVW
CSB-BP804177BVW
CSB-MP804177BVW
CSB-EP804177BVW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium japonicum Acetylglutamate kinase (argB) CSB-YP804178BVW
CSB-EP804178BVW
CSB-BP804178BVW
CSB-MP804178BVW
CSB-EP804178BVW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium japonicum Phosphonates import ATP-binding protein PhnC (phnC) CSB-YP804179BVW
CSB-EP804179BVW
CSB-BP804179BVW
CSB-MP804179BVW
CSB-EP804179BVW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium japonicum Isoleucine--tRNA ligase (ileS), partial CSB-YP804180BVW
CSB-EP804180BVW
CSB-BP804180BVW
CSB-MP804180BVW
CSB-EP804180BVW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium japonicum Prolipoprotein diacylglyceryl transferase (lgt), partial CSB-YP804181BVW1
CSB-EP804181BVW1
CSB-BP804181BVW1
CSB-MP804181BVW1
CSB-EP804181BVW1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium japonicum Protein TolB (tolB) CSB-YP804182BVW
CSB-EP804182BVW
CSB-BP804182BVW
CSB-MP804182BVW
CSB-EP804182BVW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium japonicum Periplasmic nitrate reductase (napA), partial CSB-YP804183BVW
CSB-EP804183BVW
CSB-BP804183BVW
CSB-MP804183BVW
CSB-EP804183BVW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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