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重組蛋白

產品名稱 貨號 規(guī)格
Recombinant Bradyrhizobium japonicum 50S ribosomal protein L31 (rpmE) CSB-YP805141BVW
CSB-EP805141BVW
CSB-BP805141BVW
CSB-MP805141BVW
CSB-EP805141BVW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium japonicum Urease subunit alpha (ureC), partial CSB-YP805142BVW
CSB-EP805142BVW
CSB-BP805142BVW
CSB-MP805142BVW
CSB-EP805142BVW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium japonicum Beta- (1-->2)glucan export ATP-binding/permease protein NdvA (ndvA), partial CSB-YP805143BVW1
CSB-EP805143BVW1
CSB-BP805143BVW1
CSB-MP805143BVW1
CSB-EP805143BVW1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium japonicum ATP synthase subunit a (atpB), partial CSB-YP805144BVW1
CSB-EP805144BVW1
CSB-BP805144BVW1
CSB-MP805144BVW1
CSB-EP805144BVW1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium japonicum Acetylornithine aminotransferase 1 (argD1) CSB-YP805145BVW
CSB-EP805145BVW
CSB-BP805145BVW
CSB-MP805145BVW
CSB-EP805145BVW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium japonicum Undecaprenyl pyrophosphate synthase 1 (uppS1) CSB-YP805146BVW
CSB-EP805146BVW
CSB-BP805146BVW
CSB-MP805146BVW
CSB-EP805146BVW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium japonicum Probable multidrug resistance protein norM (norM), partial CSB-YP805147BVW
CSB-EP805147BVW
CSB-BP805147BVW
CSB-MP805147BVW
CSB-EP805147BVW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium japonicum Translation initiation factor IF-2 (infB), partial CSB-YP805148BVW
CSB-EP805148BVW
CSB-BP805148BVW
CSB-MP805148BVW
CSB-EP805148BVW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium japonicum Ubiquinone/menaquinone biosynthesis methyltransferase ubiE (ubiE) CSB-YP805149BVW
CSB-EP805149BVW
CSB-BP805149BVW
CSB-MP805149BVW
CSB-EP805149BVW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium japonicum Integration host factor subunit beta (ihfB) CSB-YP805150BVW
CSB-EP805150BVW
CSB-BP805150BVW
CSB-MP805150BVW
CSB-EP805150BVW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium japonicum 2,3-bisphosphoglycerate-dependent phosphoglycerate mutase (gpmA) CSB-YP805151BVW
CSB-EP805151BVW
CSB-BP805151BVW
CSB-MP805151BVW
CSB-EP805151BVW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium japonicum Imidazoleglycerol-phosphate dehydratase (hisB) CSB-YP805152BVW
CSB-EP805152BVW
CSB-BP805152BVW
CSB-MP805152BVW
CSB-EP805152BVW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium japonicum tRNA modification GTPase MnmE (mnmE) CSB-YP805153BVW
CSB-EP805153BVW
CSB-BP805153BVW
CSB-MP805153BVW
CSB-EP805153BVW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium japonicum Acetyl-coenzyme A synthetase (acsA), partial CSB-YP805154BVW
CSB-EP805154BVW
CSB-BP805154BVW
CSB-MP805154BVW
CSB-EP805154BVW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium japonicum 3-isopropylmalate dehydratase small subunit (leuD) CSB-YP805155BVW
CSB-EP805155BVW
CSB-BP805155BVW
CSB-MP805155BVW
CSB-EP805155BVW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium japonicum ATP synthase subunit delta (atpH) CSB-YP805156BVW
CSB-EP805156BVW
CSB-BP805156BVW
CSB-MP805156BVW
CSB-EP805156BVW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium japonicum RNA pyrophosphohydrolase (rppH) CSB-YP805157BVW
CSB-EP805157BVW
CSB-BP805157BVW
CSB-MP805157BVW
CSB-EP805157BVW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium japonicum 50S ribosomal protein L21 (rplU) CSB-YP805158BVW
CSB-EP805158BVW
CSB-BP805158BVW
CSB-MP805158BVW
CSB-EP805158BVW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium japonicum Nitrous-oxide reductase (nosZ), partial CSB-YP805159BVW
CSB-EP805159BVW
CSB-BP805159BVW
CSB-MP805159BVW
CSB-EP805159BVW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium japonicum Release factor glutamine methyltransferase (prmC) CSB-YP805160BVW
CSB-EP805160BVW
CSB-BP805160BVW
CSB-MP805160BVW
CSB-EP805160BVW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質形式,可以通過多種方式產生大量蛋白質、修改基因序列和制造有用的商業(yè)產品。重組蛋白的技術資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經驗總結出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產,在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質合成機制來產生由該基因編碼的蛋白質。表達載體可使外源基因在宿主細胞中高效表達,經過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術。在重組蛋白生產過程中是一種基本成分。正如標題所示,本文主要總結了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質?

根據(jù)重組蛋白的不同應用,對其生物活性有不同的要求。但是我們如何確保蛋白質的產生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務,您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質有任何疑問,涉及到蛋白質的價格、交貨、質量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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