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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Mouse Tubulin-specific chaperone E (Tbce) CSB-YP807543MO
CSB-EP807543MO
CSB-BP807543MO
CSB-MP807543MO
CSB-EP807543MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Cdc42-interacting protein 4 (Trip10) CSB-YP807544MO
CSB-EP807544MO
CSB-BP807544MO
CSB-MP807544MO
CSB-EP807544MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat Zymogen granule membrane protein 16 (Zg16) CSB-BP807545RA
CSB-MP807545RA
CSB-EP807545RA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Protein argonaute-2 (Ago2), partial CSB-YP807546MO
CSB-EP807546MO
CSB-BP807546MO
CSB-MP807546MO
CSB-EP807546MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptomyces coelicolor Chaperone protein ClpB (clpB), partial CSB-YP807547FOB
CSB-EP807547FOB
CSB-BP807547FOB
CSB-MP807547FOB
CSB-EP807547FOB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptomyces coelicolor UvrABC system protein B (uvrB), partial CSB-YP807548FOB
CSB-EP807548FOB
CSB-BP807548FOB
CSB-MP807548FOB
CSB-EP807548FOB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus epidermidis Putative septation protein spoVG (spoVG) CSB-YP807549SLD
CSB-EP807549SLD
CSB-BP807549SLD
CSB-MP807549SLD
CSB-EP807549SLD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus epidermidis Serine-aspartate repeat-containing protein F (sdrF), partial CSB-YP807550SLD
CSB-EP807550SLD
CSB-BP807550SLD
CSB-MP807550SLD
CSB-EP807550SLD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus epidermidis Dihydroneopterin aldolase (folB) CSB-YP807551SLD
CSB-EP807551SLD
CSB-BP807551SLD
CSB-MP807551SLD
CSB-EP807551SLD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus epidermidis Arginine deiminase 2 (arcA2) CSB-YP807552SLD
CSB-EP807552SLD
CSB-BP807552SLD
CSB-MP807552SLD
CSB-EP807552SLD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus epidermidis Probable transglycosylase isaA (isaA) CSB-YP807553SLD
CSB-EP807553SLD
CSB-BP807553SLD
CSB-MP807553SLD
CSB-EP807553SLD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus epidermidis Fructose-1,6-bisphosphatase class 3 (fbp), partial CSB-YP807554SLD
CSB-EP807554SLD
CSB-BP807554SLD
CSB-MP807554SLD
CSB-EP807554SLD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus epidermidis 2,3-bisphosphoglycerate-dependent phosphoglycerate mutase (gpmA) CSB-YP807555SLD
CSB-EP807555SLD
CSB-BP807555SLD
CSB-MP807555SLD
CSB-EP807555SLD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus epidermidis Heme response regulator hssR (hssR) CSB-YP807556SLD
CSB-EP807556SLD
CSB-BP807556SLD
CSB-MP807556SLD
CSB-EP807556SLD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus epidermidis Urease accessory protein UreG (ureG) CSB-YP807557SLD
CSB-EP807557SLD
CSB-BP807557SLD
CSB-MP807557SLD
CSB-EP807557SLD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus epidermidis Molybdopterin synthase catalytic subunit (moaE) CSB-YP807558SLD
CSB-EP807558SLD
CSB-BP807558SLD
CSB-MP807558SLD
CSB-EP807558SLD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus epidermidis ATP synthase subunit a (atpB), partial CSB-YP807559SLD1
CSB-EP807559SLD1
CSB-BP807559SLD1
CSB-MP807559SLD1
CSB-EP807559SLD1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus epidermidis ATP synthase subunit beta (atpD) CSB-YP807560SLD
CSB-EP807560SLD
CSB-BP807560SLD
CSB-MP807560SLD
CSB-EP807560SLD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus epidermidis Cardiolipin synthase 2 (cls2), partial CSB-YP807561SLD1
CSB-EP807561SLD1
CSB-BP807561SLD1
CSB-MP807561SLD1
CSB-EP807561SLD1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus epidermidis 2-isopropylmalate synthase (leuA), partial CSB-YP807562SLD
CSB-EP807562SLD
CSB-BP807562SLD
CSB-MP807562SLD
CSB-EP807562SLD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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