Your Good Partner in Biology Research

重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Human immunodeficiency virus type 2 subtype B Envelope glycoprotein gp160 (env), partial CSB-YP807123HME
CSB-EP807123HME
CSB-BP807123HME
CSB-MP807123HME
CSB-EP807123HME-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Fowl adenovirus A serotype 1 Deoxyuridine 5'-triphosphate nucleotidohydrolase (1) CSB-YP807124FCL
CSB-EP807124FCL
CSB-BP807124FCL
CSB-MP807124FCL
CSB-EP807124FCL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant tRNA-specific 2-thiouridylase mnmA 1 (mnmA1) CSB-YP807125CMN
CSB-EP807125CMN
CSB-BP807125CMN
CSB-MP807125CMN
CSB-EP807125CMN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Glutamate--tRNA ligase (gltX) CSB-YP807126CMN
CSB-EP807126CMN
CSB-BP807126CMN
CSB-MP807126CMN
CSB-EP807126CMN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cassava vein mosaic virus Putative enzymatic polyprotein (ORF 3), partial CSB-YP807127CGAE
CSB-EP807127CGAE
CSB-BP807127CGAE
CSB-MP807127CGAE
CSB-EP807127CGAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Influenza A virus Nuclear export protein (NS) CSB-YP807128IIM
CSB-EP807128IIM
CSB-BP807128IIM
CSB-MP807128IIM
CSB-EP807128IIM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human immunodeficiency virus type 2 subtype B Protein Tat (tat) CSB-YP807129HME
CSB-EP807129HME
CSB-BP807129HME
CSB-MP807129HME
CSB-EP807129HME-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant African swine fever virus Envelope protein p54 (Mal-134), partial CSB-YP807130AYE1
CSB-EP807130AYE1
CSB-BP807130AYE1
CSB-MP807130AYE1
CSB-EP807130AYE1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant tRNA (guanine-N (7)-)-methyltransferase (trmB) CSB-YP807131CMN
CSB-EP807131CMN
CSB-BP807131CMN
CSB-MP807131CMN
CSB-EP807131CMN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cobalt transport protein CbiM (cbiM), partial CSB-YP807132CMN1
CSB-EP807132CMN1
CSB-BP807132CMN1
CSB-MP807132CMN1
CSB-EP807132CMN1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Canine oral papillomavirus Protein E6 (E6) CSB-YP807133CJAO
CSB-EP807133CJAO
CSB-BP807133CJAO
CSB-MP807133CJAO
CSB-EP807133CJAO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human herpesvirus 6A Uncharacterized protein U78 (U78) CSB-YP807134HJZ
CSB-EP807134HJZ
CSB-BP807134HJZ
CSB-MP807134HJZ
CSB-EP807134HJZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Avian infectious bronchitis virus Envelope small membrane protein (E), partial CSB-YP807135ARN1
CSB-EP807135ARN1
CSB-BP807135ARN1
CSB-MP807135ARN1
CSB-EP807135ARN1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 5-dehydro-2-deoxygluconokinase (iolC) CSB-YP807136CMN
CSB-EP807136CMN
CSB-BP807136CMN
CSB-MP807136CMN
CSB-EP807136CMN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 2,3-bisphosphoglycerate-independent phosphoglycerate mutase (gpmI), partial CSB-YP807137CMN
CSB-EP807137CMN
CSB-BP807137CMN
CSB-MP807137CMN
CSB-EP807137CMN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant UDP-N-acetylglucosamine 1-carboxyvinyltransferase 2 (murA2) CSB-YP807138CMN
CSB-EP807138CMN
CSB-BP807138CMN
CSB-MP807138CMN
CSB-EP807138CMN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant African swine fever virus Putative ATP-dependent RNA helicase Q706L (Mal-130), partial CSB-YP807139AYE
CSB-EP807139AYE
CSB-BP807139AYE
CSB-MP807139AYE
CSB-EP807139AYE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methionine--tRNA ligase (metG), partial CSB-YP807140CMN
CSB-EP807140CMN
CSB-BP807140CMN
CSB-MP807140CMN
CSB-EP807140CMN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Foldase protein prsA (prsA) CSB-YP807141CMN
CSB-EP807141CMN
CSB-BP807141CMN
CSB-MP807141CMN
CSB-EP807141CMN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ribosomal RNA large subunit methyltransferase H (rlmH) CSB-YP807142CMN
CSB-EP807142CMN
CSB-BP807142CMN
CSB-MP807142CMN
CSB-EP807142CMN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

Read More >>