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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Bacteroides thetaiotaomicron Homoserine O-succinyltransferase (metA) CSB-YP806289BDU
CSB-EP806289BDU
CSB-BP806289BDU
CSB-MP806289BDU
CSB-EP806289BDU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacteroides thetaiotaomicron UPF0102 protein BT_2236 (BT_2236) CSB-YP806290BDU
CSB-EP806290BDU
CSB-BP806290BDU
CSB-MP806290BDU
CSB-EP806290BDU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacteroides thetaiotaomicron Chorismate synthase (aroC) CSB-YP806291BDU
CSB-EP806291BDU
CSB-BP806291BDU
CSB-MP806291BDU
CSB-EP806291BDU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacteroides thetaiotaomicron Guanylate kinase (gmk) CSB-YP806292BDU
CSB-EP806292BDU
CSB-BP806292BDU
CSB-MP806292BDU
CSB-EP806292BDU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacteroides thetaiotaomicron UPF0324 membrane protein BT_1919 (BT_1919), partial CSB-YP806293BDU
CSB-EP806293BDU
CSB-BP806293BDU
CSB-MP806293BDU
CSB-EP806293BDU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacteroides thetaiotaomicron 60 kDa chaperonin (groL), partial CSB-YP806294BDU
CSB-EP806294BDU
CSB-BP806294BDU
CSB-MP806294BDU
CSB-EP806294BDU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacteroides thetaiotaomicron Phenylalanine--tRNA ligase alpha subunit (pheS) CSB-YP806295BDU
CSB-EP806295BDU
CSB-BP806295BDU
CSB-MP806295BDU
CSB-EP806295BDU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacteroides thetaiotaomicron Malonyl-CoA O-methyltransferase BioC (bioC) CSB-YP806296BDU
CSB-EP806296BDU
CSB-BP806296BDU
CSB-MP806296BDU
CSB-EP806296BDU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacteroides thetaiotaomicron Diaminopimelate decarboxylase (lysA) CSB-YP806297BDU
CSB-EP806297BDU
CSB-BP806297BDU
CSB-MP806297BDU
CSB-EP806297BDU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacteroides thetaiotaomicron Fructose-1,6-bisphosphatase class 3 (fbp), partial CSB-YP806298BDU
CSB-EP806298BDU
CSB-BP806298BDU
CSB-MP806298BDU
CSB-EP806298BDU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacteroides thetaiotaomicron 2,3-bisphosphoglycerate-dependent phosphoglycerate mutase 1 (gpmA1) CSB-YP806299BDU
CSB-EP806299BDU
CSB-BP806299BDU
CSB-MP806299BDU
CSB-EP806299BDU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacteroides thetaiotaomicron Proline--tRNA ligase (proS) CSB-YP806300BDU
CSB-EP806300BDU
CSB-BP806300BDU
CSB-MP806300BDU
CSB-EP806300BDU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacteroides thetaiotaomicron tRNA (guanine-N (1)-)-methyltransferase (trmD) CSB-YP806301BDU
CSB-EP806301BDU
CSB-BP806301BDU
CSB-MP806301BDU
CSB-EP806301BDU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacteroides thetaiotaomicron Isoleucine--tRNA ligase (ileS), partial CSB-YP806302BDU
CSB-EP806302BDU
CSB-BP806302BDU
CSB-MP806302BDU
CSB-EP806302BDU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacteroides thetaiotaomicron Uncharacterized transporter BT_0736 (BT_0736), partial CSB-YP806303BDU
CSB-EP806303BDU
CSB-BP806303BDU
CSB-MP806303BDU
CSB-EP806303BDU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacteroides thetaiotaomicron Hydroxylamine reductase (hcp), partial CSB-YP806304BDU
CSB-EP806304BDU
CSB-BP806304BDU
CSB-MP806304BDU
CSB-EP806304BDU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacteroides thetaiotaomicron Elongation factor 4 (lepA), partial CSB-YP806305BDU
CSB-EP806305BDU
CSB-BP806305BDU
CSB-MP806305BDU
CSB-EP806305BDU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacteroides thetaiotaomicron LL-diaminopimelate aminotransferase (dapL) CSB-YP806306BDU
CSB-EP806306BDU
CSB-BP806306BDU
CSB-MP806306BDU
CSB-EP806306BDU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacteroides thetaiotaomicron Xanthine phosphoribosyltransferase (xpt) CSB-YP806307BDU
CSB-EP806307BDU
CSB-BP806307BDU
CSB-MP806307BDU
CSB-EP806307BDU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacteroides thetaiotaomicron Ribosomal protein L11 methyltransferase (prmA) CSB-YP806308BDU
CSB-EP806308BDU
CSB-BP806308BDU
CSB-MP806308BDU
CSB-EP806308BDU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質形式,可以通過多種方式產(chǎn)生大量蛋白質、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質合成機制來產(chǎn)生由該基因編碼的蛋白質。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質?

根據(jù)重組蛋白的不同應用,對其生物活性有不同的要求。但是我們如何確保蛋白質的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務,您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質有任何疑問,涉及到蛋白質的價格、交貨、質量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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