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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant UDP-N-acetylglucosamine--N-acetylmuramyl- (pentapeptide) pyrophosphoryl-undecaprenol N-acetylglucosamine transferase (murG) CSB-YP808243EGX
CSB-EP808243EGX
CSB-BP808243EGX
CSB-MP808243EGX
CSB-EP808243EGX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ribulokinase (araB) CSB-YP808244EGX
CSB-EP808244EGX
CSB-BP808244EGX
CSB-MP808244EGX
CSB-EP808244EGX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 4-hydroxythreonine-4-phosphate dehydrogenase (pdxA) CSB-YP808245EGX
CSB-EP808245EGX
CSB-BP808245EGX
CSB-MP808245EGX
CSB-EP808245EGX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Probable crotonobetaine/carnitine-CoA ligase (caiC) CSB-YP808246EGX
CSB-EP808246EGX
CSB-BP808246EGX
CSB-MP808246EGX
CSB-EP808246EGX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Isoleucine--tRNA ligase (ileS), partial CSB-YP808247EGX
CSB-EP808247EGX
CSB-BP808247EGX
CSB-MP808247EGX
CSB-EP808247EGX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Homoserine kinase (thrB) CSB-YP808248EGX
CSB-EP808248EGX
CSB-BP808248EGX
CSB-MP808248EGX
CSB-EP808248EGX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant UPF0371 protein CE2832 (CE2832) CSB-YP808249CQG
CSB-EP808249CQG
CSB-BP808249CQG
CSB-MP808249CQG
CSB-EP808249CQG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Serine--tRNA ligase (serS) CSB-YP808250CQG
CSB-EP808250CQG
CSB-BP808250CQG
CSB-MP808250CQG
CSB-EP808250CQG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chaperone protein ClpB (clpB), partial CSB-YP808251CQG
CSB-EP808251CQG
CSB-BP808251CQG
CSB-MP808251CQG
CSB-EP808251CQG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant UDP-N-acetylglucosamine 1-carboxyvinyltransferase (murA) CSB-YP808252CQG
CSB-EP808252CQG
CSB-BP808252CQG
CSB-MP808252CQG
CSB-EP808252CQG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Glutamate racemase (murI) CSB-YP808253CQG
CSB-EP808253CQG
CSB-BP808253CQG
CSB-MP808253CQG
CSB-EP808253CQG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oligoribonuclease (orn) CSB-YP808254CQG
CSB-EP808254CQG
CSB-BP808254CQG
CSB-MP808254CQG
CSB-EP808254CQG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Valine--tRNA ligase (valS), partial CSB-YP808255CQG
CSB-EP808255CQG
CSB-BP808255CQG
CSB-MP808255CQG
CSB-EP808255CQG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Glutamate 5-kinase (proB) CSB-YP808256CQG
CSB-EP808256CQG
CSB-BP808256CQG
CSB-MP808256CQG
CSB-EP808256CQG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Elongation factor 4 (lepA), partial CSB-YP808257CQG
CSB-EP808257CQG
CSB-BP808257CQG
CSB-MP808257CQG
CSB-EP808257CQG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Glutamate-ammonia-ligase adenylyltransferase (glnE), partial CSB-YP808258CQG
CSB-EP808258CQG
CSB-BP808258CQG
CSB-MP808258CQG
CSB-EP808258CQG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cytochrome c oxidase subunit 3 (ctaE), partial CSB-YP808259CQG1
CSB-EP808259CQG1
CSB-BP808259CQG1
CSB-MP808259CQG1
CSB-EP808259CQG1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Probable menaquinol-cytochrome c reductase cytochrome b subunit (qcrB), partial CSB-YP808260CQG
CSB-EP808260CQG
CSB-BP808260CQG
CSB-MP808260CQG
CSB-EP808260CQG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Histidinol dehydrogenase (hisD) CSB-YP808261CQG
CSB-EP808261CQG
CSB-BP808261CQG
CSB-MP808261CQG
CSB-EP808261CQG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prolipoprotein diacylglyceryl transferase (lgt), partial CSB-YP808262CQG1
CSB-EP808262CQG1
CSB-BP808262CQG1
CSB-MP808262CQG1
CSB-EP808262CQG1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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