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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Staphylococcus epidermidis Protein grpE (grpE) CSB-YP809331SLD
CSB-EP809331SLD
CSB-BP809331SLD
CSB-MP809331SLD
CSB-EP809331SLD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus epidermidis Elongation factor P (efp) CSB-YP809332SLD
CSB-EP809332SLD
CSB-BP809332SLD
CSB-MP809332SLD
CSB-EP809332SLD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus epidermidis Pyrroline-5-carboxylate reductase (proC) CSB-YP809333SLD
CSB-EP809333SLD
CSB-BP809333SLD
CSB-MP809333SLD
CSB-EP809333SLD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus epidermidis 3-dehydroquinate synthase (aroB) CSB-YP809334SLD
CSB-EP809334SLD
CSB-BP809334SLD
CSB-MP809334SLD
CSB-EP809334SLD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus epidermidis 2-oxoglutarate dehydrogenase E1 component (odhA), partial CSB-YP809335SLD
CSB-EP809335SLD
CSB-BP809335SLD
CSB-MP809335SLD
CSB-EP809335SLD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus epidermidis Phosphate import ATP-binding protein PstB (pstB) CSB-YP809336SLD
CSB-EP809336SLD
CSB-BP809336SLD
CSB-MP809336SLD
CSB-EP809336SLD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus epidermidis Large-conductance mechanosensitive channel (mscL), partial CSB-YP809337SLD
CSB-EP809337SLD
CSB-BP809337SLD
CSB-MP809337SLD
CSB-EP809337SLD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus epidermidis Ribosome-binding factor A (rbfA) CSB-YP809338SLD
CSB-EP809338SLD
CSB-BP809338SLD
CSB-MP809338SLD
CSB-EP809338SLD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus epidermidis Methylenetetrahydrofolate--tRNA- (uracil-5-)-methyltransferase TrmFO (trmFO) CSB-YP809339SLD
CSB-EP809339SLD
CSB-BP809339SLD
CSB-MP809339SLD
CSB-EP809339SLD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus epidermidis 3-oxoacyl-[acyl-carrier-protein] reductase FabG (fabG) CSB-YP809340SLD
CSB-EP809340SLD
CSB-BP809340SLD
CSB-MP809340SLD
CSB-EP809340SLD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus epidermidis Cell division protein FtsA (ftsA) CSB-YP809341SLD
CSB-EP809341SLD
CSB-BP809341SLD
CSB-MP809341SLD
CSB-EP809341SLD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus epidermidis Pyruvate dehydrogenase E1 component subunit alpha (pdhA) CSB-YP809342SLD
CSB-EP809342SLD
CSB-BP809342SLD
CSB-MP809342SLD
CSB-EP809342SLD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus epidermidis N5-carboxyaminoimidazole ribonucleotide synthase (purK) CSB-YP809343SLD
CSB-EP809343SLD
CSB-BP809343SLD
CSB-MP809343SLD
CSB-EP809343SLD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus epidermidis Probable glycolipid permease ltaA (ltaA), partial CSB-YP809344SLD
CSB-EP809344SLD
CSB-BP809344SLD
CSB-MP809344SLD
CSB-EP809344SLD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus epidermidis Na (+)/H (+) antiporter subunit D1 (mnhD1), partial CSB-YP809345SLD
CSB-EP809345SLD
CSB-BP809345SLD
CSB-MP809345SLD
CSB-EP809345SLD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus epidermidis Glycine cleavage system H protein (gcvH) CSB-YP809346SLD
CSB-EP809346SLD
CSB-BP809346SLD
CSB-MP809346SLD
CSB-EP809346SLD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus epidermidis Enolase (eno) CSB-YP809347SLD
CSB-EP809347SLD
CSB-BP809347SLD
CSB-MP809347SLD
CSB-EP809347SLD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus epidermidis Protein translocase subunit SecA 1 (secA1), partial CSB-YP809348SLD
CSB-EP809348SLD
CSB-BP809348SLD
CSB-MP809348SLD
CSB-EP809348SLD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus epidermidis Malate dehydrogenase (mdh) CSB-YP809349SLD
CSB-EP809349SLD
CSB-BP809349SLD
CSB-MP809349SLD
CSB-EP809349SLD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus epidermidis Putative antiporter subunit mnhE2 (mnhE2), partial CSB-YP809350SLD1
CSB-EP809350SLD1
CSB-BP809350SLD1
CSB-MP809350SLD1
CSB-EP809350SLD1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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