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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Arabidopsis thaliana PTI1-like tyrosine-protein kinase 1 (PTI11) CSB-YP808443DOA
CSB-EP808443DOA
CSB-BP808443DOA
CSB-MP808443DOA
CSB-EP808443DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Inositol oxygenase 4 (MIOX4) CSB-YP808444DOA
CSB-EP808444DOA
CSB-BP808444DOA
CSB-MP808444DOA
CSB-EP808444DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Anaphase-promoting complex subunit 2 (APC2), partial CSB-YP808445DOA
CSB-EP808445DOA
CSB-BP808445DOA
CSB-MP808445DOA
CSB-EP808445DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Artemisia vulgaris Profilin-1 CSB-YP808446AOH
CSB-EP808446AOH
CSB-BP808446AOH
CSB-MP808446AOH
CSB-EP808446AOH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica SPX domain-containing protein 6 (SPX6) CSB-YP808447OFG
CSB-EP808447OFG
CSB-BP808447OFG
CSB-MP808447OFG
CSB-EP808447OFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica Potassium transporter 7 (HAK7), partial CSB-YP808448OFG
CSB-EP808448OFG
CSB-BP808448OFG
CSB-MP808448OFG
CSB-EP808448OFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica B3 domain-containing protein Os07g0183200 (Os07g0183200, LOC_Os07g08530) CSB-YP808449OFG
CSB-EP808449OFG
CSB-BP808449OFG
CSB-MP808449OFG
CSB-EP808449OFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica Probable aquaporin PIP2-1 (PIP2-1), partial CSB-YP808450OFG1
CSB-EP808450OFG1
CSB-BP808450OFG1
CSB-MP808450OFG1
CSB-EP808450OFG1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Phleum pratense Pollen allergen Phl p 11 CSB-YP808451EUQ
CSB-EP808451EUQ
CSB-BP808451EUQ
CSB-MP808451EUQ
CSB-EP808451EUQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica Coatomer subunit gamma-1 (Os03g0227000, LOC_Os03g12590), partial CSB-YP808452OFG
CSB-EP808452OFG
CSB-BP808452OFG
CSB-MP808452OFG
CSB-EP808452OFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica Protein IN2-1 homolog B (GSTZ5) CSB-YP808453OFG
CSB-EP808453OFG
CSB-BP808453OFG
CSB-MP808453OFG
CSB-EP808453OFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterobacteria phage ST64T DNA transfer protein gp7 (7) CSB-YP808454EDAJ
CSB-EP808454EDAJ
CSB-BP808454EDAJ
CSB-MP808454EDAJ
CSB-EP808454EDAJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Varecia variegata variegata NADH-ubiquinone oxidoreductase chain 4L (MT-ND4L), partial CSB-YP808455VEK1
CSB-EP808455VEK1
CSB-BP808455VEK1
CSB-MP808455VEK1
CSB-EP808455VEK1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clyomys laticeps Cytochrome b (MT-CYB), partial CSB-YP808456CJAQ
CSB-EP808456CJAQ
CSB-BP808456CJAQ
CSB-MP808456CJAQ
CSB-EP808456CJAQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cryphonectria parasitica NADH-ubiquinone oxidoreductase chain 4L (ND4L), partial CSB-YP808457DYH1
CSB-EP808457DYH1
CSB-BP808457DYH1
CSB-MP808457DYH1
CSB-EP808457DYH1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Schizosaccharomyces octosporus Cytochrome b (cob), partial CSB-YP808458SDP
CSB-EP808458SDP
CSB-BP808458SDP
CSB-MP808458SDP
CSB-EP808458SDP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Plecotus auritus Cytochrome b (MT-CYB), partial CSB-YP808459PIAV
CSB-EP808459PIAV
CSB-BP808459PIAV
CSB-MP808459PIAV
CSB-EP808459PIAV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pinus pinaster Maturase K (matK), partial CSB-YP808460PKM
CSB-EP808460PKM
CSB-BP808460PKM
CSB-MP808460PKM
CSB-EP808460PKM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pinus taeda Maturase K (matK), partial CSB-YP808461EVY
CSB-EP808461EVY
CSB-BP808461EVY
CSB-MP808461EVY
CSB-EP808461EVY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Schisandra chinensis Photosystem II reaction center protein T (psbT), partial CSB-YP808462SGAW
CSB-EP808462SGAW
CSB-BP808462SGAW
CSB-MP808462SGAW
CSB-EP808462SGAW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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