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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Oceanobacillus iheyensis DNA ligase (ligA), partial CSB-YP809431OAB
CSB-EP809431OAB
CSB-BP809431OAB
CSB-MP809431OAB
CSB-EP809431OAB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oceanobacillus iheyensis Histidine biosynthesis bifunctional protein HisIE (hisI) CSB-YP809432OAB
CSB-EP809432OAB
CSB-BP809432OAB
CSB-MP809432OAB
CSB-EP809432OAB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma penetrans tRNA pseudouridine synthase A (truA) CSB-YP809433MLU
CSB-EP809433MLU
CSB-BP809433MLU
CSB-MP809433MLU
CSB-EP809433MLU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leptospira interrogans serogroup Icterohaemorrhagiae serovar Lai tRNA dimethylallyltransferase (miaA) CSB-YP809434LOS
CSB-EP809434LOS
CSB-BP809434LOS
CSB-MP809434LOS
CSB-EP809434LOS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant HTH-type transcriptional regulator malT (malT), partial CSB-YP809435EGX
CSB-EP809435EGX
CSB-BP809435EGX
CSB-MP809435EGX
CSB-EP809435EGX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 60 kDa chaperonin 1 (groL1), partial CSB-YP809436CQG
CSB-EP809436CQG
CSB-BP809436CQG
CSB-MP809436CQG
CSB-EP809436CQG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella suis biovar 1 tRNA dimethylallyltransferase (miaA) CSB-YP809437BMQ
CSB-EP809437BMQ
CSB-BP809437BMQ
CSB-MP809437BMQ
CSB-EP809437BMQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pneumoniae Plasmin and fibronectin-binding protein A (pfbA), partial CSB-YP809438SMP
CSB-EP809438SMP
CSB-BP809438SMP
CSB-MP809438SMP
CSB-EP809438SMP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oceanobacillus iheyensis 2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate synthase (menD), partial CSB-YP809439OAB
CSB-EP809439OAB
CSB-BP809439OAB
CSB-MP809439OAB
CSB-EP809439OAB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant His operon leader peptide (hisL) CSB-YP809440YAS
CSB-EP809440YAS
CSB-BP809440YAS
CSB-MP809440YAS
CSB-EP809440YAS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acetyl-coenzyme A synthetase (acs), partial CSB-YP809441YAS
CSB-EP809441YAS
CSB-BP809441YAS
CSB-MP809441YAS
CSB-EP809441YAS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant GMP synthase [glutamine-hydrolyzing] (guaA), partial CSB-YP809442WAN
CSB-EP809442WAN
CSB-BP809442WAN
CSB-MP809442WAN
CSB-EP809442WAN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Coproporphyrinogen-III oxidase, aerobic (hemF) CSB-YP809443WAN
CSB-EP809443WAN
CSB-BP809443WAN
CSB-MP809443WAN
CSB-EP809443WAN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 50S ribosomal protein L17 (rplQ) CSB-YP809444WAN
CSB-EP809444WAN
CSB-BP809444WAN
CSB-MP809444WAN
CSB-EP809444WAN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 30S ribosomal protein S5 (rpsE) CSB-YP809445WAN
CSB-EP809445WAN
CSB-BP809445WAN
CSB-MP809445WAN
CSB-EP809445WAN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 50S ribosomal protein L14 (rplN) CSB-YP809446WAN
CSB-EP809446WAN
CSB-BP809446WAN
CSB-MP809446WAN
CSB-EP809446WAN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 30S ribosomal protein S19 (rpsS) CSB-YP809447WAN
CSB-EP809447WAN
CSB-BP809447WAN
CSB-MP809447WAN
CSB-EP809447WAN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ferrochelatase (hemH) CSB-YP809448WAN
CSB-EP809448WAN
CSB-BP809448WAN
CSB-MP809448WAN
CSB-EP809448WAN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 50S ribosomal protein L10 (rplJ) CSB-YP809449WAN
CSB-EP809449WAN
CSB-BP809449WAN
CSB-MP809449WAN
CSB-EP809449WAN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Single-stranded DNA-binding protein (ssb) CSB-YP809450WAN
CSB-EP809450WAN
CSB-BP809450WAN
CSB-MP809450WAN
CSB-EP809450WAN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務,您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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