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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Bifidobacterium longum Proline--tRNA ligase (proS), partial CSB-YP808343BGP
CSB-EP808343BGP
CSB-BP808343BGP
CSB-MP808343BGP
CSB-EP808343BGP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bifidobacterium longum Inosine-5'-monophosphate dehydrogenase (guaB), partial CSB-YP808344BGP
CSB-EP808344BGP
CSB-BP808344BGP
CSB-MP808344BGP
CSB-EP808344BGP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bifidobacterium longum Adenylate kinase (adk) CSB-YP808345BGP
CSB-EP808345BGP
CSB-BP808345BGP
CSB-MP808345BGP
CSB-EP808345BGP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bifidobacterium longum 50S ribosomal protein L5 (rplE) CSB-YP808346BGP
CSB-EP808346BGP
CSB-BP808346BGP
CSB-MP808346BGP
CSB-EP808346BGP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bifidobacterium longum 50S ribosomal protein L3 (rplC) CSB-YP808347BGP
CSB-EP808347BGP
CSB-BP808347BGP
CSB-MP808347BGP
CSB-EP808347BGP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bifidobacterium longum 50S ribosomal protein L7/L12 (rplL) CSB-YP808348BGP
CSB-EP808348BGP
CSB-BP808348BGP
CSB-MP808348BGP
CSB-EP808348BGP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bifidobacterium longum Uridylate kinase (pyrH) CSB-YP808349BGP
CSB-EP808349BGP
CSB-BP808349BGP
CSB-MP808349BGP
CSB-EP808349BGP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bifidobacterium longum DNA translocase FtsK (ftsK), partial CSB-YP808350BGP
CSB-EP808350BGP
CSB-BP808350BGP
CSB-MP808350BGP
CSB-EP808350BGP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bifidobacterium longum D-tyrosyl-tRNA (Tyr) deacylase (dtd) CSB-YP808351BGP
CSB-EP808351BGP
CSB-BP808351BGP
CSB-MP808351BGP
CSB-EP808351BGP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bifidobacterium longum Imidazole glycerol phosphate synthase subunit HisH (hisH) CSB-YP808352BGP
CSB-EP808352BGP
CSB-BP808352BGP
CSB-MP808352BGP
CSB-EP808352BGP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bifidobacterium longum Histidinol-phosphate aminotransferase (hisC) CSB-YP808353BGP
CSB-EP808353BGP
CSB-BP808353BGP
CSB-MP808353BGP
CSB-EP808353BGP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bifidobacterium longum 50S ribosomal protein L27 (rpmA) CSB-YP808354BGP
CSB-EP808354BGP
CSB-BP808354BGP
CSB-MP808354BGP
CSB-EP808354BGP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bifidobacterium longum 3-isopropylmalate dehydratase large subunit (leuC) CSB-YP808355BGP
CSB-EP808355BGP
CSB-BP808355BGP
CSB-MP808355BGP
CSB-EP808355BGP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bifidobacterium longum DNA-directed RNA polymerase subunit beta (rpoB), partial CSB-YP808356BGP
CSB-EP808356BGP
CSB-BP808356BGP
CSB-MP808356BGP
CSB-EP808356BGP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bifidobacterium longum S-ribosylhomocysteine lyase (luxS) CSB-YP808357BGP
CSB-EP808357BGP
CSB-BP808357BGP
CSB-MP808357BGP
CSB-EP808357BGP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bifidobacterium longum 30S ribosomal protein S7 (rpsG) CSB-YP808358BGP
CSB-EP808358BGP
CSB-BP808358BGP
CSB-MP808358BGP
CSB-EP808358BGP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bifidobacterium longum Arginine biosynthesis bifunctional protein ArgJ (argJ) CSB-YP808359BGP
CSB-EP808359BGP
CSB-BP808359BGP
CSB-MP808359BGP
CSB-EP808359BGP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bifidobacterium longum Peptidyl-tRNA hydrolase (pth) CSB-YP808360BGP
CSB-EP808360BGP
CSB-BP808360BGP
CSB-MP808360BGP
CSB-EP808360BGP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bifidobacterium longum Dephospho-CoA kinase (coaE) CSB-YP808361BGP
CSB-EP808361BGP
CSB-BP808361BGP
CSB-MP808361BGP
CSB-EP808361BGP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bifidobacterium longum GMP synthase [glutamine-hydrolyzing] (guaA), partial CSB-YP808362BGP
CSB-EP808362BGP
CSB-BP808362BGP
CSB-MP808362BGP
CSB-EP808362BGP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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