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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Chlorobium tepidum Triosephosphate isomerase (tpiA) CSB-YP809193DST
CSB-EP809193DST
CSB-BP809193DST
CSB-MP809193DST
CSB-EP809193DST-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlorobium tepidum Shikimate kinase (aroK) CSB-YP809194DST
CSB-EP809194DST
CSB-BP809194DST
CSB-MP809194DST
CSB-EP809194DST-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlorobium tepidum 50S ribosomal protein L33 (rpmG) CSB-YP809195DST
CSB-EP809195DST
CSB-BP809195DST
CSB-MP809195DST
CSB-EP809195DST-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlorobium tepidum UPF0761 membrane protein CT1325 (CT1325), partial CSB-YP809196DST1
CSB-EP809196DST1
CSB-BP809196DST1
CSB-MP809196DST1
CSB-EP809196DST1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlorobium tepidum Phosphoribosylformylglycinamidine synthase 2 (purL), partial CSB-YP809197DST
CSB-EP809197DST
CSB-BP809197DST
CSB-MP809197DST
CSB-EP809197DST-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlorobium tepidum Ribosomal RNA large subunit methyltransferase N (rlmN) CSB-YP809198DST
CSB-EP809198DST
CSB-BP809198DST
CSB-MP809198DST
CSB-EP809198DST-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlorobium tepidum Argininosuccinate synthase (argG) CSB-YP809199DST
CSB-EP809199DST
CSB-BP809199DST
CSB-MP809199DST
CSB-EP809199DST-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlorobium tepidum Cobalamin synthase (cobS), partial CSB-YP809200DST1
CSB-EP809200DST1
CSB-BP809200DST1
CSB-MP809200DST1
CSB-EP809200DST1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlorobium tepidum UPF0133 protein CT0805 (CT0805) CSB-YP809201DST
CSB-EP809201DST
CSB-BP809201DST
CSB-MP809201DST
CSB-EP809201DST-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlorobium tepidum NADH-quinone oxidoreductase subunit B (nuoB) CSB-YP809202DST
CSB-EP809202DST
CSB-BP809202DST
CSB-MP809202DST
CSB-EP809202DST-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlorobium tepidum Thiazole synthase (thiG) CSB-YP809203DST
CSB-EP809203DST
CSB-BP809203DST
CSB-MP809203DST
CSB-EP809203DST-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlorobium tepidum Homoserine O-acetyltransferase (metX) CSB-YP809204DST
CSB-EP809204DST
CSB-BP809204DST
CSB-MP809204DST
CSB-EP809204DST-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlorobium tepidum UDP-N-acetylglucosamine 1-carboxyvinyltransferase (murA) CSB-YP809205DST
CSB-EP809205DST
CSB-BP809205DST
CSB-MP809205DST
CSB-EP809205DST-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlorobium tepidum Isoleucine--tRNA ligase (ileS), partial CSB-YP809206DST
CSB-EP809206DST
CSB-BP809206DST
CSB-MP809206DST
CSB-EP809206DST-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlorobium tepidum Holliday junction ATP-dependent DNA helicase RuvA (ruvA) CSB-YP809207DST
CSB-EP809207DST
CSB-BP809207DST
CSB-MP809207DST
CSB-EP809207DST-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlorobium tepidum Ribosome-binding factor A (rbfA) CSB-YP809208DST
CSB-EP809208DST
CSB-BP809208DST
CSB-MP809208DST
CSB-EP809208DST-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlorobium tepidum 50S ribosomal protein L10 (rplJ) CSB-YP809209DST
CSB-EP809209DST
CSB-BP809209DST
CSB-MP809209DST
CSB-EP809209DST-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlorobium tepidum 1-deoxy-D-xylulose 5-phosphate reductoisomerase (dxr) CSB-YP809210DST
CSB-EP809210DST
CSB-BP809210DST
CSB-MP809210DST
CSB-EP809210DST-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlorobium tepidum Glycerol-3-phosphate dehydrogenase [NAD (P)+] (gpsA) CSB-YP809211DST
CSB-EP809211DST
CSB-BP809211DST
CSB-MP809211DST
CSB-EP809211DST-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlorobium tepidum Cytochrome c-555 (CT0075) CSB-YP809212DST
CSB-EP809212DST
CSB-BP809212DST
CSB-MP809212DST
CSB-EP809212DST-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質形式,可以通過多種方式產(chǎn)生大量蛋白質、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質合成機制來產(chǎn)生由該基因編碼的蛋白質。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質?

根據(jù)重組蛋白的不同應用,對其生物活性有不同的要求。但是我們如何確保蛋白質的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務,您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質有任何疑問,涉及到蛋白質的價格、交貨、質量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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