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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Oceanobacillus iheyensis UDP-N-acetylmuramoylalanine--D-glutamate ligase (murD) CSB-YP808083OAB
CSB-EP808083OAB
CSB-BP808083OAB
CSB-MP808083OAB
CSB-EP808083OAB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oceanobacillus iheyensis Uncharacterized N-acetyltransferase OB1458 (OB1458) CSB-YP808084OAB
CSB-EP808084OAB
CSB-BP808084OAB
CSB-MP808084OAB
CSB-EP808084OAB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oceanobacillus iheyensis UPF0637 protein OB1420 (OB1420) CSB-YP808085OAB
CSB-EP808085OAB
CSB-BP808085OAB
CSB-MP808085OAB
CSB-EP808085OAB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oceanobacillus iheyensis N-acetyldiaminopimelate deacetylase (OB1403) CSB-YP808086OAB
CSB-EP808086OAB
CSB-BP808086OAB
CSB-MP808086OAB
CSB-EP808086OAB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oceanobacillus iheyensis UPF0477 protein OB1230 (OB1230) CSB-YP808087OAB
CSB-EP808087OAB
CSB-BP808087OAB
CSB-MP808087OAB
CSB-EP808087OAB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oceanobacillus iheyensis 3-oxoacyl-[acyl-carrier-protein] synthase 3 (fabH) CSB-YP808088OAB
CSB-EP808088OAB
CSB-BP808088OAB
CSB-MP808088OAB
CSB-EP808088OAB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oceanobacillus iheyensis Putative ABC transporter ATP-binding protein OB0804 (OB0804), partial CSB-YP808089OAB
CSB-EP808089OAB
CSB-BP808089OAB
CSB-MP808089OAB
CSB-EP808089OAB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oceanobacillus iheyensis UPF0316 protein OB0738 (OB0738), partial CSB-YP808090OAB1
CSB-EP808090OAB1
CSB-BP808090OAB1
CSB-MP808090OAB1
CSB-EP808090OAB1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oceanobacillus iheyensis Phosphonates import ATP-binding protein PhnC 1 (phnC1) CSB-YP808091OAB
CSB-EP808091OAB
CSB-BP808091OAB
CSB-MP808091OAB
CSB-EP808091OAB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oceanobacillus iheyensis 1- (5-phosphoribosyl)-5-[ (5-phosphoribosylamino)methylideneamino] imidazole-4-carboxamide isomerase (hisA) CSB-YP808092OAB
CSB-EP808092OAB
CSB-BP808092OAB
CSB-MP808092OAB
CSB-EP808092OAB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oceanobacillus iheyensis N- (5'-phosphoribosyl)anthranilate isomerase (trpF) CSB-YP808093OAB
CSB-EP808093OAB
CSB-BP808093OAB
CSB-MP808093OAB
CSB-EP808093OAB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oceanobacillus iheyensis L-rhamnose isomerase (rhaA) CSB-YP808094OAB
CSB-EP808094OAB
CSB-BP808094OAB
CSB-MP808094OAB
CSB-EP808094OAB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oceanobacillus iheyensis Putative nucleotidase OB0422 (OB0422) CSB-YP808095OAB
CSB-EP808095OAB
CSB-BP808095OAB
CSB-MP808095OAB
CSB-EP808095OAB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oceanobacillus iheyensis Putative 3-methyladenine DNA glycosylase (OB0297) CSB-YP808096OAB
CSB-EP808096OAB
CSB-BP808096OAB
CSB-MP808096OAB
CSB-EP808096OAB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oceanobacillus iheyensis Energy-coupling factor transporter ATP-binding protein EcfA 1 (ecfA1) CSB-YP808097OAB
CSB-EP808097OAB
CSB-BP808097OAB
CSB-MP808097OAB
CSB-EP808097OAB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oceanobacillus iheyensis Translation initiation factor IF-1 (infA) CSB-YP808098OAB
CSB-EP808098OAB
CSB-BP808098OAB
CSB-MP808098OAB
CSB-EP808098OAB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oceanobacillus iheyensis 50S ribosomal protein L14 (rplN) CSB-YP808099OAB
CSB-EP808099OAB
CSB-BP808099OAB
CSB-MP808099OAB
CSB-EP808099OAB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oceanobacillus iheyensis 50S ribosomal protein L4 (rplD) CSB-YP808100OAB
CSB-EP808100OAB
CSB-BP808100OAB
CSB-MP808100OAB
CSB-EP808100OAB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oceanobacillus iheyensis 50S ribosomal protein L10 (rplJ) CSB-YP808101OAB
CSB-EP808101OAB
CSB-BP808101OAB
CSB-MP808101OAB
CSB-EP808101OAB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oceanobacillus iheyensis 50S ribosomal protein L25 (rplY) CSB-YP808102OAB
CSB-EP808102OAB
CSB-BP808102OAB
CSB-MP808102OAB
CSB-EP808102OAB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細(xì)胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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