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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Shewanella oneidensis Hydroxylamine reductase (hcp), partial CSB-YP808023SGA
CSB-EP808023SGA
CSB-BP808023SGA
CSB-MP808023SGA
CSB-EP808023SGA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella oneidensis Pyridoxine 5'-phosphate synthase (pdxJ) CSB-YP808024SGA
CSB-EP808024SGA
CSB-BP808024SGA
CSB-MP808024SGA
CSB-EP808024SGA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella oneidensis Prolipoprotein diacylglyceryl transferase (lgt), partial CSB-YP808025SGA1
CSB-EP808025SGA1
CSB-BP808025SGA1
CSB-MP808025SGA1
CSB-EP808025SGA1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella oneidensis Probable tRNA threonylcarbamoyladenosine biosynthesis protein Gcp (gcp) CSB-YP808026SGA
CSB-EP808026SGA
CSB-BP808026SGA
CSB-MP808026SGA
CSB-EP808026SGA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella oneidensis Translation initiation factor IF-2 (infB), partial CSB-YP808027SGA
CSB-EP808027SGA
CSB-BP808027SGA
CSB-MP808027SGA
CSB-EP808027SGA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella oneidensis UPF0250 protein SO_1163 (SO_1163) CSB-YP808028SGA
CSB-EP808028SGA
CSB-BP808028SGA
CSB-MP808028SGA
CSB-EP808028SGA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella oneidensis DNA polymerase IV (dinB) CSB-YP808029SGA
CSB-EP808029SGA
CSB-BP808029SGA
CSB-MP808029SGA
CSB-EP808029SGA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella oneidensis Protein SlyX homolog (slyX) CSB-YP808030SGA
CSB-EP808030SGA
CSB-BP808030SGA
CSB-MP808030SGA
CSB-EP808030SGA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella oneidensis Lysine--tRNA ligase (lysS) CSB-YP808031SGA
CSB-EP808031SGA
CSB-BP808031SGA
CSB-MP808031SGA
CSB-EP808031SGA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella oneidensis Non-canonical purine NTP phosphatase (SO_0917) CSB-YP808032SGA
CSB-EP808032SGA
CSB-BP808032SGA
CSB-MP808032SGA
CSB-EP808032SGA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella oneidensis 3-methyl-2-oxobutanoate hydroxymethyltransferase (panB) CSB-YP808033SGA
CSB-EP808033SGA
CSB-BP808033SGA
CSB-MP808033SGA
CSB-EP808033SGA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella oneidensis Macrolide export ATP-binding/permease protein MacB (macB), partial CSB-YP808034SGA1
CSB-EP808034SGA1
CSB-BP808034SGA1
CSB-MP808034SGA1
CSB-EP808034SGA1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella oneidensis N-succinylglutamate 5-semialdehyde dehydrogenase (astD) CSB-YP808035SGA
CSB-EP808035SGA
CSB-BP808035SGA
CSB-MP808035SGA
CSB-EP808035SGA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella oneidensis RNA polymerase-associated protein RapA (rapA), partial CSB-YP808036SGA
CSB-EP808036SGA
CSB-BP808036SGA
CSB-MP808036SGA
CSB-EP808036SGA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella oneidensis Bifunctional purine biosynthesis protein PurH (purH), partial CSB-YP808037SGA
CSB-EP808037SGA
CSB-BP808037SGA
CSB-MP808037SGA
CSB-EP808037SGA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella oneidensis DNA-binding protein fis (fis) CSB-YP808038SGA
CSB-EP808038SGA
CSB-BP808038SGA
CSB-MP808038SGA
CSB-EP808038SGA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella oneidensis Cytochrome c biogenesis ATP-binding export protein CcmA (ccmA) CSB-YP808039SGA
CSB-EP808039SGA
CSB-BP808039SGA
CSB-MP808039SGA
CSB-EP808039SGA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella oneidensis 50S ribosomal protein L6 (rplF) CSB-YP808040SGA
CSB-EP808040SGA
CSB-BP808040SGA
CSB-MP808040SGA
CSB-EP808040SGA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella oneidensis 30S ribosomal protein S17 (rpsQ) CSB-YP808041SGA
CSB-EP808041SGA
CSB-BP808041SGA
CSB-MP808041SGA
CSB-EP808041SGA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella oneidensis 50S ribosomal protein L23 (rplW) CSB-YP808042SGA
CSB-EP808042SGA
CSB-BP808042SGA
CSB-MP808042SGA
CSB-EP808042SGA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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