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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Buchnera aphidicola subsp. Schizaphis graminum 50S ribosomal protein L15 (rplO) CSB-YP809133BXE
CSB-EP809133BXE
CSB-BP809133BXE
CSB-MP809133BXE
CSB-EP809133BXE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Buchnera aphidicola subsp. Schizaphis graminum Protein smg (smg) CSB-YP809134BXE
CSB-EP809134BXE
CSB-BP809134BXE
CSB-MP809134BXE
CSB-EP809134BXE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Buchnera aphidicola subsp. Schizaphis graminum ATP-dependent Clp protease ATP-binding subunit ClpX (clpX) CSB-YP809135BXE
CSB-EP809135BXE
CSB-BP809135BXE
CSB-MP809135BXE
CSB-EP809135BXE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Buchnera aphidicola subsp. Schizaphis graminum Cytochrome o ubiquinol oxidase protein CyoD (cyoD), partial CSB-YP809136BXE1
CSB-EP809136BXE1
CSB-BP809136BXE1
CSB-MP809136BXE1
CSB-EP809136BXE1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Buchnera aphidicola subsp. Schizaphis graminum Farnesyl diphosphate synthase (ispA) CSB-YP809137BXE
CSB-EP809137BXE
CSB-BP809137BXE
CSB-MP809137BXE
CSB-EP809137BXE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Buchnera aphidicola subsp. Schizaphis graminum Exodeoxyribonuclease V beta chain (recB), partial CSB-YP809138BXE
CSB-EP809138BXE
CSB-BP809138BXE
CSB-MP809138BXE
CSB-EP809138BXE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Buchnera aphidicola subsp. Schizaphis graminum (Dimethylallyl)adenosine tRNA methylthiotransferase MiaB (miaB) CSB-YP809139BXE
CSB-EP809139BXE
CSB-BP809139BXE
CSB-MP809139BXE
CSB-EP809139BXE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Buchnera aphidicola subsp. Schizaphis graminum GTP-binding protein TypA/BipA homolog (typA), partial CSB-YP809140BXE
CSB-EP809140BXE
CSB-BP809140BXE
CSB-MP809140BXE
CSB-EP809140BXE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Buchnera aphidicola subsp. Schizaphis graminum 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase (ispD) CSB-YP809141BXE
CSB-EP809141BXE
CSB-BP809141BXE
CSB-MP809141BXE
CSB-EP809141BXE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Buchnera aphidicola subsp. Schizaphis graminum 6-carboxy-5,6,7,8-tetrahydropterin synthase (queD) CSB-YP809142BXE
CSB-EP809142BXE
CSB-BP809142BXE
CSB-MP809142BXE
CSB-EP809142BXE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Buchnera aphidicola subsp. Schizaphis graminum Carbon storage regulator homolog (csrA) CSB-YP809143BXE
CSB-EP809143BXE
CSB-BP809143BXE
CSB-MP809143BXE
CSB-EP809143BXE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Buchnera aphidicola subsp. Schizaphis graminum 3-dehydroquinate dehydratase (aroQ) CSB-YP809144BXE
CSB-EP809144BXE
CSB-BP809144BXE
CSB-MP809144BXE
CSB-EP809144BXE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Buchnera aphidicola subsp. Schizaphis graminum Ribosome maturation factor RimM (rimM) CSB-YP809145BXE
CSB-EP809145BXE
CSB-BP809145BXE
CSB-MP809145BXE
CSB-EP809145BXE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Buchnera aphidicola subsp. Schizaphis graminum GTPase obg (obg) CSB-YP809146BXE
CSB-EP809146BXE
CSB-BP809146BXE
CSB-MP809146BXE
CSB-EP809146BXE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Buchnera aphidicola subsp. Schizaphis graminum Ribosomal RNA large subunit methyltransferase E (rlmE) CSB-YP809147BXE
CSB-EP809147BXE
CSB-BP809147BXE
CSB-MP809147BXE
CSB-EP809147BXE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Buchnera aphidicola subsp. Schizaphis graminum ABC transporter ATP-binding protein uup (uup), partial CSB-YP809148BXE
CSB-EP809148BXE
CSB-BP809148BXE
CSB-MP809148BXE
CSB-EP809148BXE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Buchnera aphidicola subsp. Schizaphis graminum DNA polymerase III subunit delta' (holB) CSB-YP809149BXE
CSB-EP809149BXE
CSB-BP809149BXE
CSB-MP809149BXE
CSB-EP809149BXE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Buchnera aphidicola subsp. Schizaphis graminum 50S ribosomal protein L32 (rpmF) CSB-YP809150BXE
CSB-EP809150BXE
CSB-BP809150BXE
CSB-MP809150BXE
CSB-EP809150BXE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Buchnera aphidicola subsp. Schizaphis graminum Flagellar P-ring protein (flgI) CSB-YP809151BXE
CSB-EP809151BXE
CSB-BP809151BXE
CSB-MP809151BXE
CSB-EP809151BXE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Buchnera aphidicola subsp. Schizaphis graminum Ribosomal RNA small subunit methyltransferase C (rsmC) CSB-YP809152BXE
CSB-EP809152BXE
CSB-BP809152BXE
CSB-MP809152BXE
CSB-EP809152BXE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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