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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Shewanella oneidensis Purine nucleoside phosphorylase DeoD-type (deoD3) CSB-YP808003SGA
CSB-EP808003SGA
CSB-BP808003SGA
CSB-MP808003SGA
CSB-EP808003SGA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella oneidensis Translation initiation factor IF-1 (infA) CSB-YP808004SGA
CSB-EP808004SGA
CSB-BP808004SGA
CSB-MP808004SGA
CSB-EP808004SGA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella oneidensis Methionine--tRNA ligase (metG), partial CSB-YP808005SGA
CSB-EP808005SGA
CSB-BP808005SGA
CSB-MP808005SGA
CSB-EP808005SGA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella oneidensis Ribonuclease HI (rnhA) CSB-YP808006SGA
CSB-EP808006SGA
CSB-BP808006SGA
CSB-MP808006SGA
CSB-EP808006SGA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella oneidensis 7-cyano-7-deazaguanine synthase (queC) CSB-YP808007SGA
CSB-EP808007SGA
CSB-BP808007SGA
CSB-MP808007SGA
CSB-EP808007SGA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella oneidensis tRNA (cmo5U34)-methyltransferase (cmoA) CSB-YP808008SGA
CSB-EP808008SGA
CSB-BP808008SGA
CSB-MP808008SGA
CSB-EP808008SGA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella oneidensis 3-demethylubiquinone-9 3-methyltransferase (ubiG) CSB-YP808009SGA
CSB-EP808009SGA
CSB-BP808009SGA
CSB-MP808009SGA
CSB-EP808009SGA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella oneidensis Chemotaxis response regulator protein-glutamate methylesterase of group 3 operon (cheB3) CSB-YP808010SGA
CSB-EP808010SGA
CSB-BP808010SGA
CSB-MP808010SGA
CSB-EP808010SGA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella oneidensis 50S ribosomal protein L35 (rpmI) CSB-YP808011SGA
CSB-EP808011SGA
CSB-BP808011SGA
CSB-MP808011SGA
CSB-EP808011SGA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella oneidensis Lipoprotein-releasing system ATP-binding protein LolD (lolD) CSB-YP808012SGA
CSB-EP808012SGA
CSB-BP808012SGA
CSB-MP808012SGA
CSB-EP808012SGA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella oneidensis Nucleoid-associated protein SO_2177 (SO_2177) CSB-YP808013SGA
CSB-EP808013SGA
CSB-BP808013SGA
CSB-MP808013SGA
CSB-EP808013SGA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella oneidensis Probable hydrogenase nickel incorporation protein HypA (hypA) CSB-YP808014SGA
CSB-EP808014SGA
CSB-BP808014SGA
CSB-MP808014SGA
CSB-EP808014SGA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella oneidensis Imidazole glycerol phosphate synthase subunit HisH (hisH) CSB-YP808015SGA
CSB-EP808015SGA
CSB-BP808015SGA
CSB-MP808015SGA
CSB-EP808015SGA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella oneidensis Adenylate kinase (adk) CSB-YP808016SGA
CSB-EP808016SGA
CSB-BP808016SGA
CSB-MP808016SGA
CSB-EP808016SGA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella oneidensis UvrABC system protein C (uvrC), partial CSB-YP808017SGA
CSB-EP808017SGA
CSB-BP808017SGA
CSB-MP808017SGA
CSB-EP808017SGA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella oneidensis Putative reductase SO_1800 (SO_1800) CSB-YP808018SGA
CSB-EP808018SGA
CSB-BP808018SGA
CSB-MP808018SGA
CSB-EP808018SGA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella oneidensis Putative pterin-4-alpha-carbinolamine dehydratase (SO_1667) CSB-YP808019SGA
CSB-EP808019SGA
CSB-BP808019SGA
CSB-MP808019SGA
CSB-EP808019SGA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella oneidensis (3R)-hydroxymyristoyl-[acyl-carrier-protein] dehydratase (fabZ) CSB-YP808020SGA
CSB-EP808020SGA
CSB-BP808020SGA
CSB-MP808020SGA
CSB-EP808020SGA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella oneidensis Recombination-associated protein RdgC (rdgC) CSB-YP808021SGA
CSB-EP808021SGA
CSB-BP808021SGA
CSB-MP808021SGA
CSB-EP808021SGA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella oneidensis Glucose-1-phosphate adenylyltransferase (glgC) CSB-YP808022SGA
CSB-EP808022SGA
CSB-BP808022SGA
CSB-MP808022SGA
CSB-EP808022SGA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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