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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Ribonuclease BN (rbn) CSB-YP808203EGX
CSB-EP808203EGX
CSB-BP808203EGX
CSB-MP808203EGX
CSB-EP808203EGX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Probable 4-amino-4-deoxy-L-arabinose-phosphoundecaprenol flippase subunit ArnF (arnF), partial CSB-YP808204EGX1
CSB-EP808204EGX1
CSB-BP808204EGX1
CSB-MP808204EGX1
CSB-EP808204EGX1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Undecaprenyl-phosphate 4-deoxy-4-formamido-L-arabinose transferase (arnC), partial CSB-YP808205EGX1
CSB-EP808205EGX1
CSB-BP808205EGX1
CSB-MP808205EGX1
CSB-EP808205EGX1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cytochrome c biogenesis ATP-binding export protein CcmA (ccmA) CSB-YP808206EGX
CSB-EP808206EGX
CSB-BP808206EGX
CSB-MP808206EGX
CSB-EP808206EGX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nickel/cobalt efflux system rcnA (rcnA), partial CSB-YP808207EGX1
CSB-EP808207EGX1
CSB-BP808207EGX1
CSB-MP808207EGX1
CSB-EP808207EGX1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Histidine biosynthesis bifunctional protein HisIE (hisI) CSB-YP808208EGX
CSB-EP808208EGX
CSB-BP808208EGX
CSB-MP808208EGX
CSB-EP808208EGX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Histidinol dehydrogenase (hisD) CSB-YP808209EGX
CSB-EP808209EGX
CSB-BP808209EGX
CSB-MP808209EGX
CSB-EP808209EGX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Alpha,alpha-trehalose-phosphate synthase [UDP-forming] (otsA) CSB-YP808210EGX
CSB-EP808210EGX
CSB-BP808210EGX
CSB-MP808210EGX
CSB-EP808210EGX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aspartate--tRNA ligase (aspS) CSB-YP808211EGX
CSB-EP808211EGX
CSB-BP808211EGX
CSB-MP808211EGX
CSB-EP808211EGX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Uncharacterized protein yobH (yobH) CSB-YP808212EGX
CSB-EP808212EGX
CSB-BP808212EGX
CSB-MP808212EGX
CSB-EP808212EGX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant UPF0249 protein ChbG (chbG) CSB-YP808213EGX
CSB-EP808213EGX
CSB-BP808213EGX
CSB-MP808213EGX
CSB-EP808213EGX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Phospho-2-dehydro-3-deoxyheptonate aldolase, Trp-sensitive (aroH) CSB-YP808214EGX
CSB-EP808214EGX
CSB-BP808214EGX
CSB-MP808214EGX
CSB-EP808214EGX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cysteine desulfurase (sufS) CSB-YP808215EGX
CSB-EP808215EGX
CSB-BP808215EGX
CSB-MP808215EGX
CSB-EP808215EGX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pyridoxamine kinase (pdxY) CSB-YP808216EGX
CSB-EP808216EGX
CSB-BP808216EGX
CSB-MP808216EGX
CSB-EP808216EGX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Voltage-gated ClC-type chloride channel ClcB (clcB), partial CSB-YP808217EGX
CSB-EP808217EGX
CSB-BP808217EGX
CSB-MP808217EGX
CSB-EP808217EGX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant UPF0056 inner membrane protein marC (marC), partial CSB-YP808218EGX1
CSB-EP808218EGX1
CSB-BP808218EGX1
CSB-MP808218EGX1
CSB-EP808218EGX1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Gamma-aminobutyraldehyde dehydrogenase (prr) CSB-YP808219EGX
CSB-EP808219EGX
CSB-BP808219EGX
CSB-MP808219EGX
CSB-EP808219EGX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant GTP cyclohydrolase-2 (ribA) CSB-YP808220EGX
CSB-EP808220EGX
CSB-BP808220EGX
CSB-MP808220EGX
CSB-EP808220EGX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Uncharacterized protein yciY (yciY) CSB-YP808221EGX
CSB-EP808221EGX
CSB-BP808221EGX
CSB-MP808221EGX
CSB-EP808221EGX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sensor protein PhoQ (phoQ), partial CSB-YP808222EGX1
CSB-EP808222EGX1
CSB-BP808222EGX1
CSB-MP808222EGX1
CSB-EP808222EGX1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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