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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Nicotiana tabacum Benzyl alcohol O-benzoyltransferase (HSR201) CSB-YP808403NHE
CSB-EP808403NHE
CSB-BP808403NHE
CSB-MP808403NHE
CSB-EP808403NHE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pisum sativum Protein TIC 40, chloroplastic (TIC40), partial CSB-YP808404EWE1
CSB-EP808404EWE1
CSB-BP808404EWE1
CSB-MP808404EWE1
CSB-EP808404EWE1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Solanum lycopersicum Succinyl-CoA ligase [ADP-forming] subunit alpha-1, mitochondrial (SCOA) CSB-YP808405SZY
CSB-EP808405SZY
CSB-BP808405SZY
CSB-MP808405SZY
CSB-EP808405SZY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Hordeum vulgare Protein Barley B recombinant (BBR) CSB-YP808406HWQ
CSB-EP808406HWQ
CSB-BP808406HWQ
CSB-MP808406HWQ
CSB-EP808406HWQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Cysteine-rich repeat secretory protein 2 (CRRSP2) CSB-YP808407DOA
CSB-EP808407DOA
CSB-BP808407DOA
CSB-MP808407DOA
CSB-EP808407DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Phosphoinositide phospholipase C 6 (PLC6), partial CSB-YP808408DOA
CSB-EP808408DOA
CSB-BP808408DOA
CSB-MP808408DOA
CSB-EP808408DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Phytophthora infestans Serine/threonine-protein kinase PKZ1 (PKZ1) CSB-YP808409PJO
CSB-EP808409PJO
CSB-BP808409PJO
CSB-MP808409PJO
CSB-EP808409PJO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica Putative zinc finger CCCH domain-containing protein 48 (Os07g0139000, LOC_Os07g04650) CSB-YP808410OFG
CSB-EP808410OFG
CSB-BP808410OFG
CSB-MP808410OFG
CSB-EP808410OFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Cytokinin riboside 5'-monophosphate phosphoribohydrolase LOG7 (LOG7) CSB-YP808411DOA
CSB-EP808411DOA
CSB-BP808411DOA
CSB-MP808411DOA
CSB-EP808411DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Probable cyclic nucleotide-gated ion channel 12 (CNGC12), partial CSB-YP808412DOA1
CSB-EP808412DOA1
CSB-BP808412DOA1
CSB-MP808412DOA1
CSB-EP808412DOA1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Gibberellin-regulated protein 9 (GASA9) CSB-YP808413DOA
CSB-EP808413DOA
CSB-BP808413DOA
CSB-MP808413DOA
CSB-EP808413DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana B3 domain-containing protein At1g08985 (At1g08985) CSB-YP808414DOA
CSB-EP808414DOA
CSB-BP808414DOA
CSB-MP808414DOA
CSB-EP808414DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana F-box/kelch-repeat protein SKIP25 (SKIP25) CSB-YP808415DOA
CSB-EP808415DOA
CSB-BP808415DOA
CSB-MP808415DOA
CSB-EP808415DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Probable anion transporter 2, chloroplastic (ANTR2), partial CSB-YP808416DOA
CSB-EP808416DOA
CSB-BP808416DOA
CSB-MP808416DOA
CSB-EP808416DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Probable pectinesterase/pectinesterase inhibitor 23 (PME23), partial CSB-YP808417DOA
CSB-EP808417DOA
CSB-BP808417DOA
CSB-MP808417DOA
CSB-EP808417DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Potassium channel AKT6 (AKT6), partial CSB-YP808418DOA
CSB-EP808418DOA
CSB-BP808418DOA
CSB-MP808418DOA
CSB-EP808418DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Histone deacetylase 15 (HDA15), partial CSB-YP808419DOA
CSB-EP808419DOA
CSB-BP808419DOA
CSB-MP808419DOA
CSB-EP808419DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana MATE efflux family protein 7 (DTXL3), partial CSB-YP808420DOA
CSB-EP808420DOA
CSB-BP808420DOA
CSB-MP808420DOA
CSB-EP808420DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Uncharacterized protein At2g39910 (At2g39910) CSB-YP808421DOA
CSB-EP808421DOA
CSB-BP808421DOA
CSB-MP808421DOA
CSB-EP808421DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Beta-glucosidase 29 (BGLU29), partial CSB-YP808422DOA
CSB-EP808422DOA
CSB-BP808422DOA
CSB-MP808422DOA
CSB-EP808422DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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