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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Maf-like protein yceF (yceF) CSB-YP808223EGX
CSB-EP808223EGX
CSB-BP808223EGX
CSB-MP808223EGX
CSB-EP808223EGX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Glyoxylate/hydroxypyruvate reductase A (ghrA) CSB-YP808224EGX
CSB-EP808224EGX
CSB-BP808224EGX
CSB-MP808224EGX
CSB-EP808224EGX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant FMN reductase (NADH) RutF (rutF) CSB-YP808225EGX
CSB-EP808225EGX
CSB-BP808225EGX
CSB-MP808225EGX
CSB-EP808225EGX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 3-hydroxydecanoyl-[acyl-carrier-protein] dehydratase (fabA) CSB-YP808226EGX
CSB-EP808226EGX
CSB-BP808226EGX
CSB-MP808226EGX
CSB-EP808226EGX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 23S rRNA (uracil (747)-C (5))-methyltransferase RlmC (rlmC) CSB-YP808227EGX
CSB-EP808227EGX
CSB-BP808227EGX
CSB-MP808227EGX
CSB-EP808227EGX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Glutathione transport system permease protein gsiC (gsiC), partial CSB-YP808228EGX1
CSB-EP808228EGX1
CSB-BP808228EGX1
CSB-MP808228EGX1
CSB-EP808228EGX1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ribosomal RNA large subunit methyltransferase F (rlmF) CSB-YP808229EGX
CSB-EP808229EGX
CSB-BP808229EGX
CSB-MP808229EGX
CSB-EP808229EGX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 8-amino-7-oxononanoate synthase (bioF) CSB-YP808230EGX
CSB-EP808230EGX
CSB-BP808230EGX
CSB-MP808230EGX
CSB-EP808230EGX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Potassium-transporting ATPase B chain (kdpB), partial CSB-YP808231EGX1
CSB-EP808231EGX1
CSB-BP808231EGX1
CSB-MP808231EGX1
CSB-EP808231EGX1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leucine--tRNA ligase (leuS), partial CSB-YP808232EGX
CSB-EP808232EGX
CSB-BP808232EGX
CSB-MP808232EGX
CSB-EP808232EGX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Apo-citrate lyase phosphoribosyl-dephospho-CoA transferase (citX) CSB-YP808233EGX
CSB-EP808233EGX
CSB-BP808233EGX
CSB-MP808233EGX
CSB-EP808233EGX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cation efflux system protein CusA (cusA), partial CSB-YP808234EGX
CSB-EP808234EGX
CSB-BP808234EGX
CSB-MP808234EGX
CSB-EP808234EGX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ureidoglycolate hydrolase (allA) CSB-YP808235EGX
CSB-EP808235EGX
CSB-BP808235EGX
CSB-MP808235EGX
CSB-EP808235EGX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant tRNA sulfurtransferase (thiI) CSB-YP808236EGX
CSB-EP808236EGX
CSB-BP808236EGX
CSB-MP808236EGX
CSB-EP808236EGX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Anti-adapter protein iraP (iraP) CSB-YP808237EGX
CSB-EP808237EGX
CSB-BP808237EGX
CSB-MP808237EGX
CSB-EP808237EGX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 50S ribosomal protein L36 2 (rpmJ2) CSB-YP808238EGX
CSB-EP808238EGX
CSB-BP808238EGX
CSB-MP808238EGX
CSB-EP808238EGX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sigma factor-binding protein crl (crl) CSB-YP808239EGX
CSB-EP808239EGX
CSB-BP808239EGX
CSB-MP808239EGX
CSB-EP808239EGX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant UPF0401 protein c0279 (c0279) CSB-YP808240EGX
CSB-EP808240EGX
CSB-BP808240EGX
CSB-MP808240EGX
CSB-EP808240EGX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 2,3,4,5-tetrahydropyridine-2,6-dicarboxylate N-succinyltransferase (dapD) CSB-YP808241EGX
CSB-EP808241EGX
CSB-BP808241EGX
CSB-MP808241EGX
CSB-EP808241EGX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Glutamate-1-semialdehyde 2,1-aminomutase (hemL) CSB-YP808242EGX
CSB-EP808242EGX
CSB-BP808242EGX
CSB-MP808242EGX
CSB-EP808242EGX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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