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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Arabidopsis thaliana Transmembrane ascorbate ferrireductase 1 (CYB561A), partial CSB-YP809253DOA1
CSB-EP809253DOA1
CSB-BP809253DOA1
CSB-MP809253DOA1
CSB-EP809253DOA1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Uncharacterized protein At5g64816 (At5g64816) CSB-YP809254DOA
CSB-EP809254DOA
CSB-BP809254DOA
CSB-MP809254DOA
CSB-EP809254DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana CASP-like protein At4g15630 (At4g15630), partial CSB-YP809255DOA1
CSB-EP809255DOA1
CSB-BP809255DOA1
CSB-MP809255DOA1
CSB-EP809255DOA1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Pyridoxal biosynthesis protein PDX1.3 (PDX13) CSB-YP809256
CSB-EP809256
CSB-BP809256
CSB-MP809256
CSB-EP809256-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Conserved oligomeric Golgi complex subunit 3 (Cog3), partial CSB-YP809295MO
CSB-EP809295MO
CSB-BP809295MO
CSB-MP809295MO
CSB-EP809295MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Protein mab-21-like 3 (Mab21L3) CSB-YP809296MO
CSB-EP809296MO
CSB-BP809296MO
CSB-MP809296MO
CSB-EP809296MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Coiled-coil domain-containing protein 132 (Ccdc132), partial CSB-YP809297MO
CSB-EP809297MO
CSB-BP809297MO
CSB-MP809297MO
CSB-EP809297MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Fermitin family homolog 2 (Fermt2) CSB-YP809298MO
CSB-EP809298MO
CSB-BP809298MO
CSB-MP809298MO
CSB-EP809298MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase gamma-2 (Plcg2), partial CSB-YP809299MO
CSB-EP809299MO
CSB-BP809299MO
CSB-MP809299MO
CSB-EP809299MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse CUGBP Elav-like family member 3 (Celf3) CSB-YP809300MO
CSB-EP809300MO
CSB-BP809300MO
CSB-MP809300MO
CSB-EP809300MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Caspase recruitment domain-containing protein 11 (Card11), partial CSB-YP809301MO
CSB-EP809301MO
CSB-BP809301MO
CSB-MP809301MO
CSB-EP809301MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Twinkle protein, mitochondrial (Peo1) CSB-YP809302MO
CSB-EP809302MO
CSB-BP809302MO
CSB-MP809302MO
CSB-EP809302MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat G-protein coupled receptor 64 (Gpr64), partial CSB-YP809303RA
CSB-EP809303RA
CSB-BP809303RA
CSB-MP809303RA
CSB-EP809303RA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Abnormal spindle-like microcephaly-associated protein homolog (Aspm), partial CSB-YP809304MO
CSB-EP809304MO
CSB-BP809304MO
CSB-MP809304MO
CSB-EP809304MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat Period circadian protein homolog 3 (Per3), partial CSB-YP809305RA
CSB-EP809305RA
CSB-BP809305RA
CSB-MP809305RA
CSB-EP809305RA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Protein argonaute-1 (Eif2c1), partial CSB-YP809306MO
CSB-EP809306MO
CSB-BP809306MO
CSB-MP809306MO
CSB-EP809306MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Testis-specific H1 histone (H1fnt) CSB-YP809307MO
CSB-EP809307MO
CSB-BP809307MO
CSB-MP809307MO
CSB-EP809307MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Phosphopentomutase (deoB1) CSB-YP809308SMI
CSB-EP809308SMI
CSB-BP809308SMI
CSB-MP809308SMI
CSB-EP809308SMI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus epidermidis Membrane protein insertase YidC 1 (yidC1), partial CSB-YP809309SLD1
CSB-EP809309SLD1
CSB-BP809309SLD1
CSB-MP809309SLD1
CSB-EP809309SLD1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus epidermidis UDP-N-acetylglucosamine--N-acetylmuramyl- (pentapeptide) pyrophosphoryl-undecaprenol N-acetylglucosamine transferase (murG) CSB-YP809310SLD
CSB-EP809310SLD
CSB-BP809310SLD
CSB-MP809310SLD
CSB-EP809310SLD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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