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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Streptococcus pneumoniae DegV domain-containing protein spr0652 (spr0652) CSB-YP809631SMP
CSB-EP809631SMP
CSB-BP809631SMP
CSB-MP809631SMP
CSB-EP809631SMP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pneumoniae UDP-N-acetylglucosamine--N-acetylmuramyl- (pentapeptide) pyrophosphoryl-undecaprenol N-acetylglucosamine transferase (murG) CSB-YP809632SMP
CSB-EP809632SMP
CSB-BP809632SMP
CSB-MP809632SMP
CSB-EP809632SMP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pneumoniae Cysteine--tRNA ligase (cysS) CSB-YP809633SMP
CSB-EP809633SMP
CSB-BP809633SMP
CSB-MP809633SMP
CSB-EP809633SMP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pneumoniae Ribosome-binding factor A (rbfA) CSB-YP809634SMP
CSB-EP809634SMP
CSB-BP809634SMP
CSB-MP809634SMP
CSB-EP809634SMP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pneumoniae MutS2 protein (mutS2), partial CSB-YP809635SMP
CSB-EP809635SMP
CSB-BP809635SMP
CSB-MP809635SMP
CSB-EP809635SMP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pneumoniae Phospho-N-acetylmuramoyl-pentapeptide-transferase (mraY), partial CSB-YP809636SMP
CSB-EP809636SMP
CSB-BP809636SMP
CSB-MP809636SMP
CSB-EP809636SMP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pneumoniae Holliday junction ATP-dependent DNA helicase RuvA (ruvA) CSB-YP809637SMP
CSB-EP809637SMP
CSB-BP809637SMP
CSB-MP809637SMP
CSB-EP809637SMP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pneumoniae Bifunctional purine biosynthesis protein PurH (purH), partial CSB-YP809638SMP
CSB-EP809638SMP
CSB-BP809638SMP
CSB-MP809638SMP
CSB-EP809638SMP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pneumoniae tRNA (Ile)-lysidine synthase (tilS) CSB-YP809639SMP
CSB-EP809639SMP
CSB-BP809639SMP
CSB-MP809639SMP
CSB-EP809639SMP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant tRNA-specific 2-thiouridylase mnmA (mnmA) CSB-YP809640FMR
CSB-EP809640FMR
CSB-BP809640FMR
CSB-MP809640FMR
CSB-EP809640FMR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant DNA mismatch repair protein mutL (mutL), partial CSB-YP809641FMR
CSB-EP809641FMR
CSB-BP809641FMR
CSB-MP809641FMR
CSB-EP809641FMR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant D-tyrosyl-tRNA (Tyr) deacylase (dtd) CSB-YP809642FMR
CSB-EP809642FMR
CSB-BP809642FMR
CSB-MP809642FMR
CSB-EP809642FMR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 30S ribosomal protein S17 (rpsQ) CSB-YP809643FMR
CSB-EP809643FMR
CSB-BP809643FMR
CSB-MP809643FMR
CSB-EP809643FMR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Adenylate kinase (adk) CSB-YP809644FMR
CSB-EP809644FMR
CSB-BP809644FMR
CSB-MP809644FMR
CSB-EP809644FMR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant DNA-directed RNA polymerase subunit beta (rpoB), partial CSB-YP809645FMR
CSB-EP809645FMR
CSB-BP809645FMR
CSB-MP809645FMR
CSB-EP809645FMR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 30S ribosomal protein S6 (rpsF) CSB-YP809646FMR
CSB-EP809646FMR
CSB-BP809646FMR
CSB-MP809646FMR
CSB-EP809646FMR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Holo-[acyl-carrier-protein] synthase (acpS) CSB-YP809647FMR
CSB-EP809647FMR
CSB-BP809647FMR
CSB-MP809647FMR
CSB-EP809647FMR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant UPF0348 protein SMU_1791c (SMU_1791c) CSB-YP809648FMR
CSB-EP809648FMR
CSB-BP809648FMR
CSB-MP809648FMR
CSB-EP809648FMR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Uncharacterized RNA methyltransferase SMU_1779c (SMU_1779c) CSB-YP809649FMR
CSB-EP809649FMR
CSB-BP809649FMR
CSB-MP809649FMR
CSB-EP809649FMR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant UPF0154 protein SMU_1719c (SMU_1719c), partial CSB-YP809650FMR
CSB-EP809650FMR
CSB-BP809650FMR
CSB-MP809650FMR
CSB-EP809650FMR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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