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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Ribosome-binding factor A (rbfA) CSB-YP809671FMR
CSB-EP809671FMR
CSB-BP809671FMR
CSB-MP809671FMR
CSB-EP809671FMR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Probable tRNA threonylcarbamoyladenosine biosynthesis protein Gcp (gcp) CSB-YP809672FMR
CSB-EP809672FMR
CSB-BP809672FMR
CSB-MP809672FMR
CSB-EP809672FMR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ribonuclease P protein component (rnpA) CSB-YP809673FMR
CSB-EP809673FMR
CSB-BP809673FMR
CSB-MP809673FMR
CSB-EP809673FMR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Polyribonucleotide nucleotidyltransferase (pnp), partial CSB-YP809674FMR
CSB-EP809674FMR
CSB-BP809674FMR
CSB-MP809674FMR
CSB-EP809674FMR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant UPF0340 protein SMU_87 (SMU_87) CSB-YP809675FMR
CSB-EP809675FMR
CSB-BP809675FMR
CSB-MP809675FMR
CSB-EP809675FMR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Holliday junction ATP-dependent DNA helicase RuvB (ruvB) CSB-YP809676FMR
CSB-EP809676FMR
CSB-BP809676FMR
CSB-MP809676FMR
CSB-EP809676FMR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Tryptophan--tRNA ligase (trpS) CSB-YP809677SMI
CSB-EP809677SMI
CSB-BP809677SMI
CSB-MP809677SMI
CSB-EP809677SMI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 50S ribosomal protein L32 (rpmF) CSB-YP809678SMI
CSB-EP809678SMI
CSB-BP809678SMI
CSB-MP809678SMI
CSB-EP809678SMI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant UPF0246 protein SAG2081 (SAG2081) CSB-YP809679SMI
CSB-EP809679SMI
CSB-BP809679SMI
CSB-MP809679SMI
CSB-EP809679SMI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Tagatose-6-phosphate kinase (lacC) CSB-YP809680SMI
CSB-EP809680SMI
CSB-BP809680SMI
CSB-MP809680SMI
CSB-EP809680SMI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ribosomal RNA small subunit methyltransferase A (rsmA) CSB-YP809681SMI
CSB-EP809681SMI
CSB-BP809681SMI
CSB-MP809681SMI
CSB-EP809681SMI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xaa-Pro dipeptidyl-peptidase (pepX), partial CSB-YP809682SMI
CSB-EP809682SMI
CSB-BP809682SMI
CSB-MP809682SMI
CSB-EP809682SMI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Glutamyl-tRNA (Gln) amidotransferase subunit A (gatA) CSB-YP809683SMI
CSB-EP809683SMI
CSB-BP809683SMI
CSB-MP809683SMI
CSB-EP809683SMI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant UDP-N-acetylmuramate--L-alanine ligase (murC) CSB-YP809684SMI
CSB-EP809684SMI
CSB-BP809684SMI
CSB-MP809684SMI
CSB-EP809684SMI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant UPF0298 protein SAG1583 (SAG1583) CSB-YP809685SMI
CSB-EP809685SMI
CSB-BP809685SMI
CSB-MP809685SMI
CSB-EP809685SMI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Peptide methionine sulfoxide reductase MsrA (msrA) CSB-YP809686SMI
CSB-EP809686SMI
CSB-BP809686SMI
CSB-MP809686SMI
CSB-EP809686SMI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cytidylate kinase (cmk) CSB-YP809687SMI
CSB-EP809687SMI
CSB-BP809687SMI
CSB-MP809687SMI
CSB-EP809687SMI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant UPF0342 protein SAG1376 (SAG1376) CSB-YP809688SMI
CSB-EP809688SMI
CSB-BP809688SMI
CSB-MP809688SMI
CSB-EP809688SMI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant ATP-dependent Clp protease ATP-binding subunit ClpX (clpX) CSB-YP809689SMI
CSB-EP809689SMI
CSB-BP809689SMI
CSB-MP809689SMI
CSB-EP809689SMI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ribose-5-phosphate isomerase A (rpiA) CSB-YP809690SMI
CSB-EP809690SMI
CSB-BP809690SMI
CSB-MP809690SMI
CSB-EP809690SMI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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