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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Thermosynechococcus elongatus 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase (ispG) CSB-YP809591TDQ
CSB-EP809591TDQ
CSB-BP809591TDQ
CSB-MP809591TDQ
CSB-EP809591TDQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermosynechococcus elongatus Ribonuclease Z (rnz) CSB-YP809592TDQ
CSB-EP809592TDQ
CSB-BP809592TDQ
CSB-MP809592TDQ
CSB-EP809592TDQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermosynechococcus elongatus Leucyl/phenylalanyl-tRNA--protein transferase (aat) CSB-YP809593TDQ
CSB-EP809593TDQ
CSB-BP809593TDQ
CSB-MP809593TDQ
CSB-EP809593TDQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermosynechococcus elongatus 33 kDa chaperonin (hslO) CSB-YP809594TDQ
CSB-EP809594TDQ
CSB-BP809594TDQ
CSB-MP809594TDQ
CSB-EP809594TDQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermosynechococcus elongatus Undecaprenyl-diphosphatase (uppP), partial CSB-YP809595TDQ
CSB-EP809595TDQ
CSB-BP809595TDQ
CSB-MP809595TDQ
CSB-EP809595TDQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermosynechococcus elongatus Cytochrome c biogenesis protein CcsB (ccsB), partial CSB-YP809596TDQ1
CSB-EP809596TDQ1
CSB-BP809596TDQ1
CSB-MP809596TDQ1
CSB-EP809596TDQ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermosynechococcus elongatus DNA-directed RNA polymerase subunit gamma (rpoC1), partial CSB-YP809597TDQ
CSB-EP809597TDQ
CSB-BP809597TDQ
CSB-MP809597TDQ
CSB-EP809597TDQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermosynechococcus elongatus 50S ribosomal protein L34 (rpmH) CSB-YP809598TDQ
CSB-EP809598TDQ
CSB-BP809598TDQ
CSB-MP809598TDQ
CSB-EP809598TDQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermosynechococcus elongatus Dihydroorotate dehydrogenase (quinone) (pyrD) CSB-YP809599TDQ
CSB-EP809599TDQ
CSB-BP809599TDQ
CSB-MP809599TDQ
CSB-EP809599TDQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermosynechococcus elongatus tRNA uridine 5-carboxymethylaminomethyl modification enzyme MnmG (mnmG), partial CSB-YP809600TDQ
CSB-EP809600TDQ
CSB-BP809600TDQ
CSB-MP809600TDQ
CSB-EP809600TDQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermosynechococcus elongatus ATP-dependent Clp protease proteolytic subunit 1 (clpP1) CSB-YP809601TDQ
CSB-EP809601TDQ
CSB-BP809601TDQ
CSB-MP809601TDQ
CSB-EP809601TDQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermosynechococcus elongatus 1,4-dihydroxy-2-naphthoyl-CoA hydrolase (tll0488) CSB-YP809602TDQ
CSB-EP809602TDQ
CSB-BP809602TDQ
CSB-MP809602TDQ
CSB-EP809602TDQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermosynechococcus elongatus ATP synthase subunit alpha (atpA), partial CSB-YP809603TDQ
CSB-EP809603TDQ
CSB-BP809603TDQ
CSB-MP809603TDQ
CSB-EP809603TDQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermosynechococcus elongatus Orotidine 5'-phosphate decarboxylase (pyrF) CSB-YP809604TDQ
CSB-EP809604TDQ
CSB-BP809604TDQ
CSB-MP809604TDQ
CSB-EP809604TDQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermosynechococcus elongatus Histidinol-phosphate aminotransferase (hisC) CSB-YP809605TDQ
CSB-EP809605TDQ
CSB-BP809605TDQ
CSB-MP809605TDQ
CSB-EP809605TDQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermosynechococcus elongatus 60 kDa chaperonin (groL), partial CSB-YP809606TDQ
CSB-EP809606TDQ
CSB-BP809606TDQ
CSB-MP809606TDQ
CSB-EP809606TDQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermosynechococcus elongatus Imidazoleglycerol-phosphate dehydratase (hisB) CSB-YP809607TDQ
CSB-EP809607TDQ
CSB-BP809607TDQ
CSB-MP809607TDQ
CSB-EP809607TDQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermosynechococcus elongatus 50S ribosomal protein L17 (rplQ) CSB-YP809608TDQ
CSB-EP809608TDQ
CSB-BP809608TDQ
CSB-MP809608TDQ
CSB-EP809608TDQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermosynechococcus elongatus 50S ribosomal protein L24 (rplX) CSB-YP809609TDQ
CSB-EP809609TDQ
CSB-BP809609TDQ
CSB-MP809609TDQ
CSB-EP809609TDQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermosynechococcus elongatus UDP-N-acetylmuramoylalanine--D-glutamate ligase (murD) CSB-YP809610TDQ
CSB-EP809610TDQ
CSB-BP809610TDQ
CSB-MP809610TDQ
CSB-EP809610TDQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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