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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Thermosynechococcus elongatus Adaptive-response sensory-kinase sasA (sasA) CSB-YP809611TDQ
CSB-EP809611TDQ
CSB-BP809611TDQ
CSB-MP809611TDQ
CSB-EP809611TDQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pneumoniae Histidine--tRNA ligase (hisS) CSB-YP809612SMP
CSB-EP809612SMP
CSB-BP809612SMP
CSB-MP809612SMP
CSB-EP809612SMP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pneumoniae Ribonuclease P protein component (rnpA) CSB-YP809613SMP
CSB-EP809613SMP
CSB-BP809613SMP
CSB-MP809613SMP
CSB-EP809613SMP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pneumoniae DNA-directed RNA polymerase subunit beta' (rpoC), partial CSB-YP809614SMP
CSB-EP809614SMP
CSB-BP809614SMP
CSB-MP809614SMP
CSB-EP809614SMP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pneumoniae Galactose-1-phosphate uridylyltransferase 1 (galT1) CSB-YP809615SMP
CSB-EP809615SMP
CSB-BP809615SMP
CSB-MP809615SMP
CSB-EP809615SMP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pneumoniae Ribosomal protein L11 methyltransferase (prmA) CSB-YP809616SMP
CSB-EP809616SMP
CSB-BP809616SMP
CSB-MP809616SMP
CSB-EP809616SMP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pneumoniae Cell division protein SepF (sepF) CSB-YP809617SMP
CSB-EP809617SMP
CSB-BP809617SMP
CSB-MP809617SMP
CSB-EP809617SMP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pneumoniae Homoserine O-succinyltransferase (metA) CSB-YP809618SMP
CSB-EP809618SMP
CSB-BP809618SMP
CSB-MP809618SMP
CSB-EP809618SMP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pneumoniae ATP synthase subunit beta (atpD) CSB-YP809619SMP
CSB-EP809619SMP
CSB-BP809619SMP
CSB-MP809619SMP
CSB-EP809619SMP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pneumoniae HPr kinase/phosphorylase (hprK) CSB-YP809620SMP
CSB-EP809620SMP
CSB-BP809620SMP
CSB-MP809620SMP
CSB-EP809620SMP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pneumoniae UPF0342 protein spr1230 (spr1230) CSB-YP809621SMP
CSB-EP809621SMP
CSB-BP809621SMP
CSB-MP809621SMP
CSB-EP809621SMP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pneumoniae Aspartate carbamoyltransferase (pyrB) CSB-YP809622SMP
CSB-EP809622SMP
CSB-BP809622SMP
CSB-MP809622SMP
CSB-EP809622SMP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pneumoniae Formate--tetrahydrofolate ligase (fhs), partial CSB-YP809623SMP
CSB-EP809623SMP
CSB-BP809623SMP
CSB-MP809623SMP
CSB-EP809623SMP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pneumoniae Tagatose-6-phosphate kinase (lacC) CSB-YP809624SMP
CSB-EP809624SMP
CSB-BP809624SMP
CSB-MP809624SMP
CSB-EP809624SMP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pneumoniae Glycogen synthase (glgA) CSB-YP809625SMP
CSB-EP809625SMP
CSB-BP809625SMP
CSB-MP809625SMP
CSB-EP809625SMP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pneumoniae Uncharacterized RNA methyltransferase spr0932 (spr0932), partial CSB-YP809626SMP
CSB-EP809626SMP
CSB-BP809626SMP
CSB-MP809626SMP
CSB-EP809626SMP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pneumoniae Dihydrodipicolinate synthase (dapA) CSB-YP809627SMP
CSB-EP809627SMP
CSB-BP809627SMP
CSB-MP809627SMP
CSB-EP809627SMP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pneumoniae SsrA-binding protein (smpB) CSB-YP809628SMP
CSB-EP809628SMP
CSB-BP809628SMP
CSB-MP809628SMP
CSB-EP809628SMP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pneumoniae Ribosome-recycling factor (frr) CSB-YP809629SMP
CSB-EP809629SMP
CSB-BP809629SMP
CSB-MP809629SMP
CSB-EP809629SMP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pneumoniae Ribose-5-phosphate isomerase A (rpiA) CSB-YP809630SMP
CSB-EP809630SMP
CSB-BP809630SMP
CSB-MP809630SMP
CSB-EP809630SMP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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