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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Escherichia coli O139:H28 Oligoribonuclease (orn) CSB-YP019586EJD
CSB-EP019586EJD
CSB-BP019586EJD
CSB-MP019586EJD
CSB-EP019586EJD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O9:H4 Queuine tRNA-ribosyltransferase (tgt) CSB-YP019146EJF
CSB-EP019146EJF
CSB-BP019146EJF
CSB-MP019146EJF
CSB-EP019146EJF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O9:H4 Protoheme IX farnesyltransferase (cyoE), partial CSB-YP005824EJF1
CSB-EP005824EJF1
CSB-BP005824EJF1
CSB-MP005824EJF1
CSB-EP005824EJF1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O9:H4 Lipoyl synthase (lipA) CSB-YP012927EJF
CSB-EP012927EJF
CSB-BP012927EJF
CSB-MP012927EJF
CSB-EP012927EJF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O9:H4 Phosphoserine aminotransferase (serC) CSB-YP018838EJF
CSB-EP018838EJF
CSB-BP018838EJF
CSB-MP018838EJF
CSB-EP018838EJF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O9:H4 Thymidylate kinase (tmk) CSB-YP007226EJF
CSB-EP007226EJF
CSB-BP007226EJF
CSB-MP007226EJF
CSB-EP007226EJF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O9:H4 Peptide chain release factor 1 (prfA) CSB-YP015197EJF
CSB-EP015197EJF
CSB-BP015197EJF
CSB-MP015197EJF
CSB-EP015197EJF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O9:H4 Serine hydroxymethyltransferase (glyA) CSB-YP021274EJF
CSB-EP021274EJF
CSB-BP021274EJF
CSB-MP021274EJF
CSB-EP021274EJF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O9:H4 Methionyl-tRNA formyltransferase (fmt) CSB-YP015150EJF
CSB-EP015150EJF
CSB-BP015150EJF
CSB-MP015150EJF
CSB-EP015150EJF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O9:H4 Elongation factor G (fusA) CSB-YP009373EJF
CSB-EP009373EJF
CSB-BP009373EJF
CSB-MP009373EJF
CSB-EP009373EJF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O9:H4 6-phosphofructokinase (pfkA) CSB-YP017822EJF
CSB-EP017822EJF
CSB-BP017822EJF
CSB-MP017822EJF
CSB-EP017822EJF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O9:H4 Glucose-6-phosphate isomerase (pgi) CSB-YP009717EJF
CSB-EP009717EJF
CSB-BP009717EJF
CSB-MP009717EJF
CSB-EP009717EJF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O9:H4 Oligoribonuclease (orn) CSB-YP019586EJF
CSB-EP019586EJF
CSB-BP019586EJF
CSB-MP019586EJF
CSB-EP019586EJF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Citrobacter koseri GTPase Era (era) CSB-YP007759CWS
CSB-EP007759CWS
CSB-BP007759CWS
CSB-MP007759CWS
CSB-EP007759CWS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Citrobacter koseri GMP synthase [glutamine-hydrolyzing] (guaA), partial CSB-YP009582CWS
CSB-EP009582CWS
CSB-BP009582CWS
CSB-MP009582CWS
CSB-EP009582CWS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Citrobacter koseri Glucokinase (glk) CSB-YP009319CWS
CSB-EP009319CWS
CSB-BP009319CWS
CSB-MP009319CWS
CSB-EP009319CWS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Citrobacter koseri Peptide chain release factor 1 (prfA) CSB-YP015197CWS
CSB-EP015197CWS
CSB-BP015197CWS
CSB-MP015197CWS
CSB-EP015197CWS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Citrobacter koseri Queuine tRNA-ribosyltransferase (tgt) CSB-YP019146CWS
CSB-EP019146CWS
CSB-BP019146CWS
CSB-MP019146CWS
CSB-EP019146CWS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Citrobacter koseri Methylthioribose-1-phosphate isomerase (mtnA) CSB-YP014799CWS
CSB-EP014799CWS
CSB-BP014799CWS
CSB-MP014799CWS
CSB-EP014799CWS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Citrobacter koseri Elongation factor G (fusA), partial CSB-YP009373CWS
CSB-EP009373CWS
CSB-BP009373CWS
CSB-MP009373CWS
CSB-EP009373CWS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務,您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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