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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Shewanella pealeana Methionyl-tRNA formyltransferase (fmt) CSB-YP015150STP
CSB-EP015150STP
CSB-BP015150STP
CSB-MP015150STP
CSB-EP015150STP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella pealeana Porphobilinogen deaminase (hemC) CSB-YP010524STP
CSB-EP010524STP
CSB-BP010524STP
CSB-MP010524STP
CSB-EP010524STP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella pealeana Phosphoserine aminotransferase (serC) CSB-YP018838STP
CSB-EP018838STP
CSB-BP018838STP
CSB-MP018838STP
CSB-EP018838STP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella pealeana Glycine cleavage system H protein (gcvH) CSB-YP009335STP
CSB-EP009335STP
CSB-BP009335STP
CSB-MP009335STP
CSB-EP009335STP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella pealeana tRNA dimethylallyltransferase (miaA) CSB-YP024529STP
CSB-EP024529STP
CSB-BP024529STP
CSB-MP024529STP
CSB-EP024529STP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella pealeana ATP synthase subunit b (atpF), partial CSB-YP002358STP1
CSB-EP002358STP1
CSB-BP002358STP1
CSB-MP002358STP1
CSB-EP002358STP1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Azorhizobium caulinodans ATP synthase subunit alpha (atpA), partial CSB-YP002344DPR
CSB-EP002344DPR
CSB-BP002344DPR
CSB-MP002344DPR
CSB-EP002344DPR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Azorhizobium caulinodans Nucleoside diphosphate kinase (ndk) CSB-YP015889DPR
CSB-EP015889DPR
CSB-BP015889DPR
CSB-MP015889DPR
CSB-EP015889DPR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Azorhizobium caulinodans ATP synthase subunit b (atpF), partial CSB-YP002358DPR1
CSB-EP002358DPR1
CSB-BP002358DPR1
CSB-MP002358DPR1
CSB-EP002358DPR1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Azorhizobium caulinodans Ribonuclease 3 (rnc) CSB-YP019809DPR
CSB-EP019809DPR
CSB-BP019809DPR
CSB-MP019809DPR
CSB-EP019809DPR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlamydomonas reinhardtii Mitochondrial cardiolipin hydrolase (CHLREDRAFT_190403), partial CSB-YP018149DSJ1
CSB-EP018149DSJ1
CSB-BP018149DSJ1
CSB-MP018149DSJ1
CSB-EP018149DSJ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Danio rerio Coiled-coil domain-containing protein 153 (ccdc153) CSB-YP004672DIL
CSB-EP004672DIL
CSB-BP004672DIL
CSB-MP004672DIL
CSB-EP004672DIL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Danio rerio Putative sodium-coupled neutral amino acid transporter 11 (slc38a11), partial CSB-YP021616DIL
CSB-EP021616DIL
CSB-BP021616DIL
CSB-MP021616DIL
CSB-EP021616DIL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dinoroseobacter shibae GMP synthase [glutamine-hydrolyzing] (guaA), partial CSB-YP009582DIB
CSB-EP009582DIB
CSB-BP009582DIB
CSB-MP009582DIB
CSB-EP009582DIB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salinispora arenicola ATP synthase subunit alpha (atpA), partial CSB-YP002344STC
CSB-EP002344STC
CSB-BP002344STC
CSB-MP002344STC
CSB-EP002344STC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Alkaliphilus oremlandii GTPase Era (era) CSB-YP007759AUQ
CSB-EP007759AUQ
CSB-BP007759AUQ
CSB-MP007759AUQ
CSB-EP007759AUQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Alkaliphilus oremlandii ATP synthase subunit beta (atpD) CSB-YP002350AUQ
CSB-EP002350AUQ
CSB-BP002350AUQ
CSB-MP002350AUQ
CSB-EP002350AUQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Coprinopsis cinerea Galectin-1 (Cgl1) CSB-YP012882DWQ
CSB-EP012882DWQ
CSB-BP012882DWQ
CSB-MP012882DWQ
CSB-EP012882DWQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brugia malayi Eukaryotic translation initiation factor 3 subunit E (Bm1_11985) CSB-YP007534BWV
CSB-EP007534BWV
CSB-BP007534BWV
CSB-MP007534BWV
CSB-EP007534BWV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brugia malayi 40S ribosomal protein S3a (Bm1_27225) CSB-YP020444BWV
CSB-EP020444BWV
CSB-BP020444BWV
CSB-MP020444BWV
CSB-EP020444BWV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務,您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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