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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Staphylococcus aureus Lipoyl synthase (lipA) CSB-YP012927SUM
CSB-EP012927SUM
CSB-BP012927SUM
CSB-MP012927SUM
CSB-EP012927SUM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Queuine tRNA-ribosyltransferase (tgt) CSB-YP019146SUM
CSB-EP019146SUM
CSB-BP019146SUM
CSB-MP019146SUM
CSB-EP019146SUM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Porphobilinogen deaminase (hemC) CSB-YP010524SUM
CSB-EP010524SUM
CSB-BP010524SUM
CSB-MP010524SUM
CSB-EP010524SUM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus 6-phosphofructokinase (pfkA) CSB-YP017822SUM
CSB-EP017822SUM
CSB-BP017822SUM
CSB-MP017822SUM
CSB-EP017822SUM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus tRNA pseudouridine synthase A (truA) CSB-YP019086SUM
CSB-EP019086SUM
CSB-BP019086SUM
CSB-MP019086SUM
CSB-EP019086SUM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Succinyl-CoA ligase [ADP-forming] subunit beta (sucC) CSB-YP022917SUM
CSB-EP022917SUM
CSB-BP022917SUM
CSB-MP022917SUM
CSB-EP022917SUM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus GTPase Era (era) CSB-YP007759SUM
CSB-EP007759SUM
CSB-BP007759SUM
CSB-MP007759SUM
CSB-EP007759SUM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sulcia muelleri ATP synthase subunit alpha (atpA), partial CSB-YP002344SUX
CSB-EP002344SUX
CSB-BP002344SUX
CSB-MP002344SUX
CSB-EP002344SUX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sulcia muelleri Elongation factor Tu (tuf) CSB-YP025342SUX
CSB-EP025342SUX
CSB-BP025342SUX
CSB-MP025342SUX
CSB-EP025342SUX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sulcia muelleri ATP synthase subunit beta (atpD) CSB-YP002350SUX
CSB-EP002350SUX
CSB-BP002350SUX
CSB-MP002350SUX
CSB-EP002350SUX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Desulfococcus oleovorans Acyl carrier protein (acpP) CSB-YP015636DHW
CSB-EP015636DHW
CSB-BP015636DHW
CSB-MP015636DHW
CSB-EP015636DHW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Desulfococcus oleovorans Peptide deformylase (def) CSB-YP017707DHW
CSB-EP017707DHW
CSB-BP017707DHW
CSB-MP017707DHW
CSB-EP017707DHW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Desulfococcus oleovorans Thymidylate kinase (tmk) CSB-YP007226DHW
CSB-EP007226DHW
CSB-BP007226DHW
CSB-MP007226DHW
CSB-EP007226DHW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Desulfococcus oleovorans Methylthioribose-1-phosphate isomerase (mtnA) CSB-YP014799DHW
CSB-EP014799DHW
CSB-BP014799DHW
CSB-MP014799DHW
CSB-EP014799DHW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salinispora arenicola Succinyl-CoA ligase [ADP-forming] subunit beta (sucC) CSB-YP022917STC
CSB-EP022917STC
CSB-BP022917STC
CSB-MP022917STC
CSB-EP022917STC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Caldivirga maquilingensis Transcription initiation factor IIB (tfb) CSB-YP010001CVY
CSB-EP010001CVY
CSB-BP010001CVY
CSB-MP010001CVY
CSB-EP010001CVY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Alkaliphilus oremlandii Ribosome-recycling factor (frr) CSB-YP014941AUQ
CSB-EP014941AUQ
CSB-BP014941AUQ
CSB-MP014941AUQ
CSB-EP014941AUQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Alkaliphilus oremlandii 6-phosphofructokinase (pfkA) CSB-YP017822AUQ
CSB-EP017822AUQ
CSB-BP017822AUQ
CSB-MP017822AUQ
CSB-EP017822AUQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Coprinopsis cinerea Flap endonuclease 1 (FEN1) CSB-YP008585DWQ
CSB-EP008585DWQ
CSB-BP008585DWQ
CSB-MP008585DWQ
CSB-EP008585DWQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brugia malayi Eukaryotic translation initiation factor 3 subunit A (Bm1_29045), partial CSB-YP007543BWV
CSB-EP007543BWV
CSB-BP007543BWV
CSB-MP007543BWV
CSB-EP007543BWV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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